Intraepithelial Lymphocyte Cytometric Pattern Is a Useful Diagnostic Tool for Coeliac Disease Diagnosis Irrespective of Degree of Mucosal Damage and Age—A Validation Cohort

Ruiz-Ramírez, Pablo; Carreras, Gerard; Fajardo, I; Tristán, Eva; Carrasco, Anna; Salvador, I; Zabana, Yamile; Andújar, Xavier; Ferrer, Carme; Horta, Diana; Loras, Carme; García-Puig, Roger; Fernández‐Bañares, Fernando; Esteve, María

doi:10.3390/nu13051684

Cited by 12 publications

(24 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notwithstanding, CD diagnosis sensitivity when using a >10% cut-off remains very high, being 87% in the ≥70 years group and 90% in the overall group. We have not evaluated the test specificity in the present study since it was very high regardless of age in previous studies [ 11 , 13 , 14 ]. We have also shown the high diagnosis accuracy of this test for CD in seronegative villous atrophy of different aetiologies [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

“…For IEL flow cytometry, one single duodenal biopsy from the second portion of the duodenum was obtained and processed immediately as previously described [ 13 ]. The results of the flow cytometry were obtained in the four hours following sample collection, which was always before pathology diagnosis.…”

Section: Methodsmentioning

confidence: 99%

“…Coeliac lymphogram was then defined as an increase in TCRγδ+ cells according to each cut-off plus a concomitant decrease in CD3− cells <10%. There were four IEL patterns: a normal pattern, an isolated decrease in CD3−, an isolated increase in TCRγδ+ and the coeliac lymphogram (an increase in TCRγδ+ plus a decrease in CD3−) [ 13 ]. The last two patterns are associated with CD.…”

Section: Methodsmentioning

confidence: 99%

“…Duodenal samples were processed using haematoxylin/eosin staining and CD3 immunophenotyping and were evaluated blind to the results of flow cytometry. Marsh 1 was considered as an IEL count of >25 IELs per 100 epithelial nuclei and normal villous architecture [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…We have demonstrated the usefulness of this tool for the diagnosis of CD in seronegative villous atrophy [ 12 ]. Accordingly, TCRγδ+ IEL count assessed by flow cytometry has been advocated as a new and accurate diagnostic tool for doubtful CD cases, irrespective of the degree of mucosal damage, sex and age [ 13 ]. However, in the aforementioned study, the number of CD patients older than 61 was very small, which precluded drawing conclusions regarding elderly people.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis

et al. 2021

Self Cite

View full text Add to dashboard Cite

(1) Background: Although a meta-analysis reported that the sensitivity of CD3+ TCRγδ+ cells for coeliac disease diagnosis was >93%, a recent study has suggested that sensitivity decreased to 65% in elderly patients. (2) Aim: To evaluate whether the sensitivity of intraepithelial lymphocyte cytometric patterns for coeliac disease diagnosis changes with advanced age. (3) Methods: We performed a multicentre study including 127 coeliac disease patients ≥ 50 years: 87 with baseline cytometry (45 aged 50–59 years; 23 aged 60–69 years; 19 aged ≥ 70 years), 16 also with a follow-up cytometry (on a gluten-free diet); and 40 with only follow-up cytometry. (4) Results: In Marsh 3 patients, a sensitivity of 94.7%, 88.9% and 86.7% was observed for each age group using a cut-off value of TCRγδ+ >10% (p = 0.27); and a sensitivity of 84.2%, 83.4% and 53.3% for a cut-off value >14% (p = 0.02; 50–69 vs. ≥70 years), with difference between applying a cut-off of 10% or 14% (p = 0.008). The TCRγδ+ count in the ≥70 years group was lower than in the other groups (p = 0.014). (5) Conclusion: In coeliac patients ≥ 70 years, the TCRγδ+ count decreases and the cut-off point of >10% is more accurate than >14%.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

et al. 2021

View full text Add to dashboard Cite

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

show abstract

Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP

Gelardi,

Giancaspro,

Duda

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Chronic rhinosinusitis with nasal polyps (CRSwNP) is defined as a Type 2 eosinophilic disease, while CRSsNP is considered a Type 1 neutrophilic disease. Since neutrophils are also activated in eosinophilic CRSwNP, the eosinophil–neutrophil dualism has been revaluated. Among the inflammatory cells infiltrating sinus-nasal tissues, the role of mast cells (MCs) is not already recognized, although Clinical-Cytological Grading, which defines the severity of CRSwNP, attributes to mixed eosinophil-MC forms of CRSwNP a greater risk of recurrence. We aimed to examine nasal polyps from both a cytological and histopathological point of view, to evaluate the presence and localization of MCs. Cytological and histological examination of 39 samples of nasal polyps were performed. Immunohistochemistry was used to evaluate the presence of Tryptase + CD117 + MCs, which were counted both in the epithelial layer and in the lamina propria. A statistically significant correlation was found between intraepithelial MCs and CRSwNP severity (p < 0.001) and between the total eosinophil count and the total mast cell count (p < 0.001). Cytological examination and immunohistochemistry were comparable in detecting the presence of intraepithelial MCs (p = 0.002). The histological cut-off of 6 intraepithelial MCs was identified to detect severe CRSwNP (p < 0.001). MCs have been shown to be located in the lamina propria of almost all eosinophilic nasal polyps without significantly affecting their severity. Intraepithelial MCs are associated with greater severity of CRSwNP. Histopathological criteria of the eosinophil-MC form of CRSwNP in addition to the eosinophilic one, should be defined to guarantee patients effective and tailored treatments.

show abstract

Intraepithelial Lymphocyte Cytometric Pattern Is a Useful Diagnostic Tool for Coeliac Disease Diagnosis Irrespective of Degree of Mucosal Damage and Age—A Validation Cohort

Cited by 12 publications

References 30 publications

Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis

Coeliac Disease in Elderly Patients: Value of Coeliac Lymphogram for Diagnosis

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Eosinophil-mast cell pattern of intraepithelial infiltration as a marker of severity in CRSwNP

Contact Info

Product

Resources

About