Intrafollicular expression and potential regulatory role of cocaine- and amphetamine-regulated transcript in the ovine ovary

Huang, Yang; Yao, Xiaolei; Meng, Jinzhu; Liu, Y.; Jiang, Xiaolong; Chen, J.W.; Li, P.F.; Ren, Youshe; Liu, W.Z.; Yao, Jianbo; Folger, Joseph K.; Smith, George W.; Lv, Lei

doi:10.1016/j.domaniend.2015.09.001

Cited by 10 publications

(8 citation statements)

References 22 publications

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“…In mammals, studies on follicles have mainly been conducted in mice [17], human [18], pig [19], bovine [20], rat [21], and sheep [22]. These studies revealed the effects of GCs and theca cells on follicular development, follicular atresia, and luteal development, and further demonstrated the mechanism of genes and signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Zou

Zhao

et al. 2020

BMC Genomics

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Background: Fertility is an important economic trait in the production of meat goat, and follicular development plays an important role in fertility. Although many mRNAs and microRNAs (miRNAs) have been found to play critical roles in ovarian biological processes, the interaction between mRNAs and miRNAs in follicular development is not yet completely understood. In addition, less attention has been given to the study of single follicle (dominant or atretic follicle) in goats. This study aimed to identify mRNAs, miRNAs, and signaling pathways as well as their interaction networks in the ovarian follicles (large follicles and small follicles) of uniparous and multiple Chuanzhong black goats at estrus phase using RNAsequencing (RNA-seq) technique. Results: The results showed that there was a significant difference in the number of large follicles between uniparous and multiple goats (P < 0.05), but no difference in the number of small follicles was observed (P > 0.05). For the small follicles of uniparous and multiple goats at estrus phase, 289 differentially expressed mRNAs (DEmRNAs) and 16 DEmiRNAs were identified; and for the large follicles, 195 DEmRNAs and 7 DEmiRNAs were identified. The functional enrichment analysis showed that DE genes in small follicles were significantly enriched in ovarian steroidogenesis and steroid hormone biosynthesis, while in large follicles were significantly enriched in ABC transporters and steroid hormone biosynthesis. The results of quantitative real-time polymerase chain reaction were consistent with those of RNA-seq. Analysis of the mRNA-miRNA interaction network suggested that CD36 (miR-122, miR-200a, miR-141), TNFAIP6 (miR-141, miR-200a, miR-182), CYP11A1 (miR-122), SERPINA5 (miR-1, miR-206, miR-133a-3p, miR-133b), and PTGFR (miR-182, miR-122) might be related to fertility, but requires further research on follicular somatic cells. Conclusions: This study was used for the first time to reveal the DEmRNAs and DEmiRNAs as well as their interaction in the follicles of uniparous and multiple goats at estrus phase using RNA-seq technology. Our findings provide new clues to uncover the molecular mechanisms and signaling networks of goat reproduction that could be potentially used to increase ovulation rate and kidding rate in goat.

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Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Zou

Zhao

et al. 2020

BMC Genomics

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“…CART treatment of ovine granulosa cells had pronounced inhibitory effects on FSH-induced E 2 production and blocked the FSH-induced increase in granulosa cells numbers observed over 7 day culture period [ 29 ], and results of studies demonstrate a similar yet distinct response of ovine granulosa cells to CART treatment as observed for the bovine system. Furthermore, recent studies support a prominent requirement of Wnt signaling for mediating stimulatory effects of FSH on E 2 production and granulosa cell proliferation [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary

Xie

Yao

et al. 2017

Biol Res

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BackgroundCocaine- and amphetamine-regulated transcript (CART), discovered initially by via differential display RT-PCR analysis of brains of rats administered cocaine, is expressed mainly in central nervous system or neuronal origin cells, and is involved in a wide range of behaviors, such as regulation of food intake, energy homeostasis, and reproduction. The hens egg-laying rate mainly depends on the developmental status of follicles, expression of CART have not been identified from hen follicles, the regulatory mechanisms of CART biological activities are still unknown. The objective of this study was to characterize the mRNA expression of CART in hen follicular granulosa cells and determine CART peptide localization and regulatory role during follicular development.MethodsSmall white follicles (1–2 mm in diameter) were treated for RNA isolation; Small white follicles (1–2 mm in diameter) and large white follicles (4–6 mm in diameter) were treated for immunohistochemical localization and large white follicles (4–6 mm in diameter), small yellow follicles (6–8 mm in diameter), large yellow follicles (9–12 mm in diameter), mature follicles (F5, F4, F3, F2, F1, >12 mm in diameter) were treated for RNA isolation and Real time PCR.ResultsThe results showed that full length of the CDS of hen CART was 336 bp encoding a 111 amino acid polypeptide. In the hen ovary, CART peptide was primarily localized to the theca layer, but not all, the oocyte and granulosa layer, with diffused, weaker staining than relative to the theca cell layer. Further, amount of CART mRNA was more (P < 0.05) in granulosa cells of 6–8 mm follicles compared with that in granulosa cells of other follicles. However, CART mRNA amount was greater in theca cells of 4–6 mm follicles relative to follicles of other sizes (P < 0.05).ConclusionsResults suggest that CART could play a potential role in developmental regulation of chicken follicles.

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“…CART plays a potential role in the sheep for follicular development regulation. It can inhibit ovine follicular growth and development (Huang et al 2016). CART can inhibit the proliferation of granulosa cells and the production of E2, which stimulated by FSH in vitro in the porcine ovarian granulosa cells, but the inhibition is not significant.…”

Section: Cocaine and Amphetamine-regulated Transcript (Cart)mentioning

confidence: 99%

Ingestive Peptides – An Emerging Tool for Diagnostics and Therapeutics: A Review

2021

Int J Vet Sci

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Peptides are relatively safe, well tolerated, highly selective and efficacious. Consequently, in recent years, peptides have acquired an increased interest as therapeutics in pharmaceutical research and development. In clinical trials, about 140 peptide therapeutics are currently being tested. In medicine and biotechnology, peptides have acquired a broad range of applications. More than 7000 peptides in nature have been recognized, and these peptides also have critical roles including actions as hormones, growth factors, neurotransmitters, ion channel ligands, or anti-infectives. Peptides are often excellent biomarkers and can be used for diagnostic purposes. Metabolic diseases and oncology are the major disease areas nowadays driving the clinical use of peptide drugs. We think that the future peptide drugs development will be a new tool for the several pathologies amelioration.

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Intrafollicular expression and potential regulatory role of cocaine- and amphetamine-regulated transcript in the ovine ovary

Cited by 10 publications

References 22 publications

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

Expression of cocaine- and amphetamine-regulated transcript (CART) in hen ovary

Ingestive Peptides – An Emerging Tool for Diagnostics and Therapeutics: A Review

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