Intrafollicular fluid metabolic abnormalities in relation to ovarian hyperstimulation syndrome: Follicular fluid metabolomics via gas chromatography-mass spectrometry

Sun, Yixuan; Hao, Lijuan; Han, Wei; Zheng, Jing; Dong, Yuan; Ye, Hong; Li, Qinke; Huang, Gangliang; Han, Ting‐Li; Yang, Zhu

doi:10.1016/j.cca.2022.11.033

Cited by 4 publications

(4 citation statements)

References 64 publications

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“…More importantly, the attenuation of COX-2 decreases the incidence of OHSS in a rat model ( 44 , 45 ). As the main fatty acid in FF ( 46 ), we found that the AA level in the FF of OHSS women was about 3 times higher than that of control patients, which is consistent with a current investigation ( 38 ). Moreover, our correlation analysis results showed that the higher AA was positively correlated with estradiol and No.…”

Section: Discussionsupporting

confidence: 92%

“…A current retrospective analysis concludes that lipid metabolism affects the morbidity of OHSS and females with dyslipidemia are more likely to develop to OHSS ( 37 ). Other two studies demonstrate that various lipids altered in FF of OHSS patients ( 22 , 38 ), indicating the significance of lipid metabolism on OHSS. Arachidonic acid (AA), an unsaturated and essential fatty acid, is produced from the phospholipids are catalyzed by phospholipase A2 (PLA2) and phospholipase C (PLC).…”

Section: Discussionmentioning

confidence: 90%

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Biomarkers identification in follicular fluid of women with OHSS by using UPLC-MS method

Fang²,

Zhang³

et al. 2023

Front. Endocrinol.

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To figure out the differentially changed metabolites and disturbed pathways in follicular fluid (FF) of patients with OHSS in comparison to the control group undergoing in vitro fertilization (IVF), we conducted this metabolomic analysis between two groups, the OHSS group included 30 patients treated with oocyte retrieval and developed OHSS in the next 7-14 days, while another 30 patients without OHSS tendency were selected as the control group. The FF samples were obtained during the process of oocyte retrieval. FF samples were analyzed using ultra-high liquid chromatography-tandem mass spectrometry (UPLC-MS). The results identified a total of 59 differentially changed metabolites, including 33 decreased metabolites (P < 0.01) and 26 increased metabolites (P < 0.01) in FF of OHSS compared with the control group. 12 metabolites could be the most valuable biomarkers for OHSS based on ROC results. Our correlation analyses showed that deoxyinosine levels were found positively correlated with serum estradiol (E2) levels in OHSS patients, while L-isoleucine, pyruvic acid, maleamate, and arachidonic acid were found to be positively correlated with the number of retrieved oocytes. Furthermore, 4-hydroxyphenylacetaldehyde, deoxycorticosterone, creatinine, and creatine were found to be negatively associated with serum E2 levels, while 4-hydroxyphenylacetaldehyde, L-carnitine, isovaleric acid and L-2-hydroxyglutaric acid were negatively related with the number of oocytes retrieved in OHSS patients. Taken together, our study provides better identification of OHSS FF metabolic dynamics, suggesting the metabolic compounds can be used as valuable predictors or treatment targets of OHSS.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 90%

Biomarkers identification in follicular fluid of women with OHSS by using UPLC-MS method

Fang²,

Zhang³

et al. 2023

Front. Endocrinol.

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“…Previous studies have highlighted significant differences in amino acid and lipid metabolites within the follicular fluid of OHSS patients compared to control groups [ 13 , 17 , 24 ]. Similarly, multiple lipid components in the serum or follicular fluid of PCOS patients exhibit alterations [ 25 , 26 ], particularly noticeable in obese individuals with PCOS, where lipid abnormalities are most pronounced [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…This exploration is essential for enhancing OHSS incidence management and improving pregnancy success rates despite the apparent effectiveness of infertility treatments/strategies, including IVF as the final step [ 16 ]. Furthermore, while some studies have identified abnormal expression of unsaturated fatty acid metabolites in follicular fluid as a risk factor for OHSS in PCOS patients using metabolomics [ 17 ], it remains unclear whether the presence of abnormally expressed metabolites in maternal serum is also associated with high ovarian response in PCOS patients.…”

Section: Introductionmentioning

confidence: 99%

Metabolic landscape and pathogenic insights: a comprehensive analysis of high ovarian response in infertile women undergoing in vitro fertilization

Ruan,

Lv,

et al. 2024

J Ovarian Res

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Background In the realm of assisted reproduction, a subset of infertile patients demonstrates high ovarian response following controlled ovarian stimulation (COS), with approximately 29.7% facing the risk of Ovarian Hyperstimulation Syndrome (OHSS). Management of OHSS risk often necessitates embryo transfer cancellation, leading to delayed prospects of successful pregnancy and significant psychological distress. Regrettably, these patients have received limited research attention, particularly regarding their metabolic profile. In this study, we aim to utilize gas chromatography-mass spectrometry (GC-MS) to reveal these patients’ unique serum metabolic profiles and provide insights into the disease’s pathogenesis. Methods We categorized 145 infertile women into two main groups: the CON infertility group from tubal infertility patients and the Polycystic Ovary Syndrome (PCOS) infertility group. Within these groups, we further subdivided them into four categories: patients with normal ovarian response (CON-NOR group), patients with high ovarian response and at risk for OHSS (CON-HOR group) within the CON group, as well as patients with normal ovarian response (PCOS-NOR group) and patients with high ovarian response and at risk for OHSS (PCOS-HOR group) within the PCOS group. Serum metabolic profiles were analyzed using GC-MS. The risk criteria for OHSS were: the number of developing follicles > 20, peak Estradiol (E2) > 4000pg/mL, and Anti-Müllerian Hormone (AMH) levels > 4.5ng/mL. Results The serum metabolomics analysis revealed four different metabolites within the CON group and 14 within the PCOS group. Remarkably, 10-pentadecenoic acid emerged as a discernible risk metabolite for the CON-HOR, also found to be a differential metabolite between CON-NOR and PCOS groups. cysteine and 5-methoxytryptamine were also identified as risk metabolites for the PCOS-HOR. Furthermore, KEGG analysis unveiled significant enrichment of the aminoacyl-tRNA biosynthesis pathway among the metabolites differing between PCOS-NOR and PCOS-HOR. Conclusion Our study highlights significant metabolite differences between patients with normal ovarian response and those with high ovarian response and at risk for OHSS within both the tubal infertility control group and PCOS infertility group. Importantly, we observe metabolic similarities between patients with PCOS and those with a high ovarian response but without PCOS, suggesting potential parallels in their underlying causes.

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Adipocyte-derived chemerin rescues lipid overload-induced cardiac dysfunction

Liu¹,

Han²,

Huang³

et al. 2023

iScience

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Intrafollicular fluid metabolic abnormalities in relation to ovarian hyperstimulation syndrome: Follicular fluid metabolomics via gas chromatography-mass spectrometry

Cited by 4 publications

References 64 publications

Biomarkers identification in follicular fluid of women with OHSS by using UPLC-MS method

Biomarkers identification in follicular fluid of women with OHSS by using UPLC-MS method

Metabolic landscape and pathogenic insights: a comprehensive analysis of high ovarian response in infertile women undergoing in vitro fertilization

Adipocyte-derived chemerin rescues lipid overload-induced cardiac dysfunction

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