Intralaboratory comparison of analytical methods for quantification of major phytocannabinoids

Béres, Tibor; Černochová, Lucie; Zeljković, Sanja Ćavar; Benická, Sandra; Gucký, Tomáš; Berčák, Michal; Tarkowski, Petr

doi:10.1007/s00216-019-01760-y

Cited by 20 publications

(27 citation statements)

References 25 publications

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“…Manual pulverization and homogenization of the dried plant material can be performed using mortar and pestle [ 46 , 47 , 48 , 49 , 50 ], metal spoon [ 51 ] or glass rod [ 52 ], by cutting the plant material [ 53 ] or crushing and riddling (0.5 mm) [ 54 ] or by manual grinder [ 50 , 55 ]. According to the UNODC [ 5 ], dried herbal cannabis material and cannabis resins should be pulverized by a cutter (at high revolution speed, i.e., 100 rps) and sieved (mesh size 1 mm).…”

Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

“…The minimization of analyte loss using manual grinder is attributed to the adhesion of cannabis resin to the blades and plastic housing surface of a plastic blender during the high-speed pulverization [ 50 ]. Mechanical grinding-activation in an intensity planetary vibrational mill [ 62 , 63 ], ball mill [ 55 ], knife mill [ 64 ] or freeze mill [ 65 ] are also applied. Instead of drying, fresh cannabis plant material can be frozen with liquid nitrogen and crushed [ 66 , 67 ] or frozen, lyophilised at −50 °C and grounded by hand [ 68 ] or in a mill [ 69 ].…”

Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

“…Instead of drying, fresh cannabis plant material can be frozen with liquid nitrogen and crushed [ 66 , 67 ] or frozen, lyophilised at −50 °C and grounded by hand [ 68 ] or in a mill [ 69 ]. Comparison of coarse homogenization by sieving through a 1-mm mesh and fine homogenization with a ball mill revealed better extraction efficiency for CBDA and THCA for the finely powdered plant material, and no difference for the neutral phytocannabinoids (CBD, Δ 9 -THC) [ 55 ].…”

Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

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Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

et al. 2022

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Cannabis is gaining increasing attention due to the high pharmacological potential and updated legislation authorizing multiple uses. The development of time- and cost-efficient analytical methods is of crucial importance for phytocannabinoid profiling. This review aims to capture the versatility of analytical methods for phytocannabinoid profiling of cannabis and cannabis-based products in the past four decades (1980–2021). The thorough overview of more than 220 scientific papers reporting different analytical techniques for phytocannabinoid profiling points out their respective advantages and drawbacks in terms of their complexity, duration, selectivity, sensitivity and robustness for their specific application, along with the most widely used sample preparation strategies. In particular, chromatographic and spectroscopic methods, are presented and discussed. Acquired knowledge of phytocannabinoid profile became extremely relevant and further enhanced chemotaxonomic classification, cultivation set-ups examination, association of medical and adverse health effects with potency and/or interplay of certain phytocannabinoids and other active constituents, quality control (QC), and stability studies, as well as development and harmonization of global quality standards. Further improvement in phytocannabinoid profiling should be focused on untargeted analysis using orthogonal analytical methods, which, joined with cheminformatics approaches for compound identification and MSLs, would lead to the identification of a multitude of new phytocannabinoids.

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Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

Section: Analytical Methods For Phytocannabinoid Profilingmentioning

confidence: 99%

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Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

et al. 2022

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“…UHPLC-MS/MS analysis of cannabinoids was performed according to the previously reported methodology [ 16 ]. Briefly, 50 ± 1 mg of each sample was weighed in triplicates.…”

Section: Methodsmentioning

confidence: 99%

Influence of Technological Maturity on the Secondary Metabolites of Hemp Concentrate (Cannabis sativa L.)

Tremlová

Mikulaskova

Hajduchová

et al. 2021

Foods

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During the last decade, the popularity of hemp products has been rising rapidly. Products containing cannabidiol (CBD) are of predominant interest. Traditional hemp products are frequently enriched by CBD due to their potential therapeutic effects. Cannabidiol occurs naturally in hemp juice together with other biologically active substances, such as terpenes, flavonoids, and stilbenoids. These constituents act synergistically. This study aimed to observe the influence of the hemp plant developmental stage on its chemical composition and antioxidant activity. The hemp plants were analyzed during three vegetative stages, i.e., before, during, and after flowering. The collected samples were evaluated using the following analyses: total polyphenolic content and profile, terpenoid and cannabinoid contents, and ferric reducing antioxidant power. The results revealed statistically significant differences between the samples in almost all set parameters. The optimal period for hemp harvest depends on desirable compounds, i.e., phenolic content is the highest before flowering, while the levels of cannabinoids and terpenoids are the highest during the flowering period.

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“…The use of UV detection is surely the most widespread because all CNBs possess chromophores in their structures, and the required sensitivity is in accordance with this detection mode 3 . Furthermore, the use of ultra‐high‐performance liquid chromatography (UHPLC) with sub‐2‐µm particles or superficially porous particles has dramatically increased the chromatographic performance in terms of analysis time, peak capacity and sensitivity 9,11,14,17 …”

Section: Introductionmentioning

confidence: 99%

The analysis of cannabinoids in cannabis samples by supercritical fluid chromatography and ultra‐high‐performance liquid chromatography: A comparison study

Deidda

Schelling

Roussel³

et al. 2020

Analytical Science Advances

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The aim of this work was to develop a supercritical fluid chromatographic method to study the applicability of this emerging technique to cannabinoid analysis and showcase its advantages. During method development, the authors focused on nine phyto‐cannabinoids to assess the selectivity needed to potentially perform the quantitation of each cannabinoid. After method development, robustness studies were carried out on this method to gain more information about its qualitative behavior (in terms of critical resolutions) when varying some crucial parameters (concentration of additive, column temperature, starting gradient conditions and column batch). Once the robustness was evaluated and the parameters most affecting the selected responses were individuated, the SFC method was applied for a simulated routine use to generate quantitative results concerning the concentrations of the main cannabinoids in real cannabis samples. The samples were also analyzed by means of an ultra‐high‐performance liquid chromatographic method currently used in the laboratory for the same objective. Finally, the results obtained with both analytical methods were compared to evaluate their accordance. The Bland–Altman method was applied as a statistical strategy to evaluate the degree of accordance between the results generated and display the data in a difference plot. The ultra‐high performance supercritical fluid chromatography quantitative results were in accordance with the ultra‐high performance liquid chromatography results, demonstrating the applicability of this technique for cannabinoid analysis.

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Intralaboratory comparison of analytical methods for quantification of major phytocannabinoids

Cited by 20 publications

References 25 publications

Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

Analytical Techniques for Phytocannabinoid Profiling of Cannabis and Cannabis-Based Products—A Comprehensive Review

Influence of Technological Maturity on the Secondary Metabolites of Hemp Concentrate (Cannabis sativa L.)

The analysis of cannabinoids in cannabis samples by supercritical fluid chromatography and ultra‐high‐performance liquid chromatography: A comparison study

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