Intralesional bleomycin A5 injection for the treatment of nasal polyps through inducing apoptosis

Tian, Peng; Wu, Fan; Wang, Jingyi; Ou, Huashuang; Xiang, Liangzhong; Chen, Qiujian; Dang, Hua; Zheng, Yiqing; Zhang, Xianghong; Zou, Hua

doi:10.1080/00016489.2017.1380312

Cited by 3 publications

(4 citation statements)

References 17 publications

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“… 30 In another study, the expression of PARP and caspase 8 were increased in bleomycin-treated NP tissue; also the tissues were decreased and then disappeared. 31 …”

Section: Discussionmentioning

confidence: 99%

High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis

Şimşek

Eren

Bahçeci

et al. 2021

Brazilian Journal of Otorhinolaryngology

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“… 30 In another study, the expression of PARP and caspase 8 were increased in bleomycin-treated NP tissue; also the tissues were decreased and then disappeared. 31 …”

Section: Discussionmentioning

confidence: 99%

High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis

Şimşek

Eren

Bahçeci

et al. 2021

Brazilian Journal of Otorhinolaryngology

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“…Moreover, we tested genotoxic and cytotoxic effects in BLE-A5-treated NPDF in our previous researches, including DNA smear testing, cell cytotoxicity assay and cell immigration assay. We found that BLE-A5 can induce DNA fragmentation, reduce cell viability and suppress proliferation in NPDFs (24,27). We also found that NPdF is more sensitive to BLE-A5 administration compared with normal nasal mucosa derived fibroblasts (NMDF) (28).…”

Section: Introductionmentioning

confidence: 75%

“…NP tissue preparation and treatment were performed as described in our previous study (24). After BLE-A5 treatment, a midsagittal section of formalin-fixed (4% at room temperature for 1 h), paraffin-embedded nasal polyp tissue was permeabilized with 0.1% Triton X-100.…”

Section: Immunofluorescence Analysis Of Np Fibroblasts and Epithelialmentioning

confidence: 99%

Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts

Wang

et al. 2020

Int J Mol Med

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Intralesional injection of bleomycin-A5 (BLE-A5) is a novel treatment for nasal polyps. Our previous study clarified that BLE-A5 could induce nasal polyp-derived fibroblast (NPDF) apoptosis in nasal polyps. However, the detailed mechanisms are still unclear. The present study aimed to determine the effects of BLE-A5 on NPDF mitochondrial dynamics and provide a theoretical basis for the local application of BLE-A5 to treat nasal polyps. In the present study, an in vitro nasal polyp tissue culture model was used to define the BLE-A5 target cell type in nasal polyps. NPDF primary cell culture was used to study the effects of BLE-A5 on the mitochondrial dynamic-related mechanism. The results showed that BLE-A5 treatment of NPDFs caused mitochondrial-mediated apoptosis. dynamin-related protein 1 (drp1) was shown to be altered in BLE-A5-treated NPDFs. Drp1 knockdown increased the sensitivity of NPDFs to BLE-A5 and exacerbated mitochondrial dysfunction. BLE-A5 decreased cyclin B1-CDK1 complex-mediated phosphorylation of Drp1 and inhibited Drp1-mediated mitophagy in NPDFs. Overall, the present study concluded that BLE-A5 mainly induces NPDF apoptosis in nasal polyps. BLE-A5 regulates the mitochondria by inhibiting Drp1 activation, resulting in NPDF mitochondrial dynamic disorder and apoptosis. Recent studies showed that the treatment of NPs with intralesional bleomycin A5 (BLE-A5) injections is safe and effective, although its underlying mechanism remains unclear (22-24). Studies on the mechanism of BLE in the

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“…Other therapies pursued in the literature for the treatment of CRSwNP, but not reviewed here, include small molecules such as verapamil, intralesional bleomycin, topical tretinoin, metformin, and phototherapy. [79][80][81][82][83]…”

Section: Additional Therapiesmentioning

confidence: 99%

State of the Art Medical Management of Nasal Polyps

Staricha

Ali

Stokken

2023

Am J Rhinol�Allergy

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Background Chronic rhinosinusitis with nasal polyposis (CRSwNP) is an inflammatory disease with a treatment goal of controlling symptoms and limiting disease burden. While endoscopic sinus surgery is effective for removing polyps and aerating sinuses, proper medical management remains necessary for reducing inflammation and limiting polyp recurrence. Objective This article aims to summarize the literature regarding medical treatment of chronic rhinosinusitis with nasal polyposis, with a specific focus on developments in the past 5 years. Methodology We conducted a literature review using PubMed to identify studies assessing medical treatment strategies for patients with CRSwNP. Articles focusing on chronic rhinosinusitis without nasal polyposis were excluded unless specifically stated. Surgical treatment and biologic therapies for CRSwNP will be covered in subsequent chapters and are therefore not included. Results Intranasal saline irrigations and topical steroids are mainstays of CRSwNP treatment in the pre-surgical, post-surgical, and maintenance phases of the disease. Alternative steroid delivery methods and adjunctive treatments with antibiotics, anti-leukotrienes, and other topical therapies have been investigated and may benefit certain patient populations, but convincing evidence does not exist to warrant addition of these treatments to the standard of care for CRSwNP. Conclusions Topical steroid therapy is clearly effective for CRSwNP, and recent studies demonstrate the safety and efficacy of high-dose nasal steroid rinses. Alternate delivery methods for local steroids may be useful for patients who are not responding to or who are noncompliant with conventional intranasal corticosteroid sprays and rinses. Future studies are needed to clarify if oral or topical antibiotics, oral anti-leukotrienes, or other novel therapies are significantly effective in decreasing symptoms and improving the quality of life in patients with CRSwNP.

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Intralesional bleomycin A5 injection for the treatment of nasal polyps through inducing apoptosis

Cited by 3 publications

References 17 publications

High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis

High PI3K/mTOR and low MAPK/JNK activity results in decreased apoptosis and autophagy in nasal polyposis

Bleomycin A5 suppresses Drp1‑mediated mitochondrial fission and induces apoptosis in human nasal polyp‑derived fibroblasts

State of the Art Medical Management of Nasal Polyps

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