Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

Sun, Huaichang; Xue, Fang-ming; Ke, Qing; Fang, Hao-xia; Qiu, Hua-lei; Zhang, Xinyu; Yin, Zhiwei

doi:10.1631/jzus.2006.b0324

Cited by 14 publications

(5 citation statements)

References 13 publications

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“…As an alternative approach, a gene therapy strategy for treating bovine mastitis was developed in 2006: a new mammary-specific vector containing hLys, cDNA, and the kanamycin resistance gene for intramammary expression and clinical studies was constructed. The clinical studies showed that the injection of the diseased quarters twice with the p215C3LYZ vector had an overt therapeutic effect on bovine mastitis (in particular, clinical mastitis), including the disappearance of symptoms, a significant improvement in the California mastitis test score, and a reasonably high microbiological cure rate [ 214 ].…”

Section: Applications Of Lysozymementioning

confidence: 99%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

209

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Lysozyme is a ~14 kDa protein present in many mucosal secretions (tears, saliva, and mucus) and tissues of animals and plants, and plays an important role in the innate immunity, providing protection against bacteria, viruses, and fungi. Three main different types of lysozymes are known: the c-type (chicken or conventional type), the g-type (goose type), and the i-type (invertebrate type). It has long been the subject of several applications due to its antimicrobial properties. The problem of antibiotic resistance has stimulated the search for new molecules or new applications of known compounds. The use of lysozyme as an alternative antibiotic is the subject of this review, which covers the results published over the past two decades. This review is focused on the applications of lysozyme in medicine, (the treatment of infectious diseases, wound healing, and anti-biofilm), veterinary, feed, food preservation, and crop protection. It is available from a wide range of sources, in addition to the well-known chicken egg white, and its synergism with other compounds, endowed with antimicrobial activity, are also summarized. An overview of the modified lysozyme applications is provided in the form of tables.

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Section: Applications Of Lysozymementioning

confidence: 99%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

209

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“…Lysostaphin transgenic cattle were also protected from an intramammary S. aureus challenge (Wall et al, 2005). A human lysozyme expressing vector for injection into cattle mammary glands has also been created and reported to reduce mastitis symptoms within days (Sun et al, 2006).…”

Section: G Food Safetymentioning

confidence: 99%

Endolysins as Antimicrobials

Nelson

Schmelcher²,

Rodríguez-Rubio

et al. 2012

Advances in Virus Research

320

330

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“…Vaccines, chemotherapeutic drugs and antibiotics of various generations are currently used for prevention and treatment of mastitis in cattle [21][22][23].…”

mentioning

confidence: 99%

ANTIBIOTIC-RESISTANCE PROFILE OF Staphylococcus aureus STRAINS ISOLATED FROM MILK OF HIGH YIELD COWS IN CENTRAL RUSSIA

Артемьева¹,

Nikanova²,

Kotkovskaya³

et al. 2016

S-h. biol.

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A b s t r a c tThe development of microbial resistance to antibiotics determines the effectiveness of the veterinary measures. The study and monitoring, well-designed treatment schemes are those measures that help to reduce the risk of spread of pathogenic and conditional-pathogenic microorganisms. One of the problems in the dairy farming is mastitis of high producing cows which is mainly caused by Staphylococcus aureus. Here we first report findings on the development of resistance in S. aureus strains isolated from milk of clinically healthy dairy cows during treatment with antibiotics of different classes. The study was carried out using high yield holsteinized black-and-white cows (n = 1321, Kaluga region, 2016) which were vaccinated twice with anti-mastitis drug Mastivak («Ovejero», Spain). Milk was sampled during the lactation period individually from each cow. For the species identification of S. aureus isolates we used the following criteria: (i) morphology and microscopy of the colonies grown on the Baird Parker Agar medium («HiMedia Laboratories Pvt., Ltd», India); (ii) the presence of hemolysis zones on the Azide Blood Agar Pronadisa medium («Conda», Spain) supplemented with 5 % defibrinated sheep blood; (iii) a coagulation of dry citrated rabbit plasma (CJSC «EKOlab», Russia); (iv) biochemical characterization with API 20 STAPH panel («BioMerieux», France). S. aureus ATCC 25923 was a reference strain. The sensitivity of the isolated microorganisms to ten antibiotics («Pharmacotherapy Research Center», Russia) including penicillin (PEN, 10, ED), oxacillin (OX, 10 μg), gentamicin (GN, 10 μg), erythromycin (ER, 15 μg), lincomycin (LN, 15 μg), rifampicin (RF, 5 μg), ciprofloxacin (CP, 5 μg), vancomycin (VA, 30 μg), fuzidin (FZ, 10 μg) and novobiocin (NB, 5 μg) was determined by disk diffusion method. A total of 104 strains among 155 isolates (67.1 %) showed resistance to one or more antibiotics. The majority of the strains were resistant to novobiocin (49.7 %) while the smallest part (2.6 %) exhibited resistant to vancomycin. Importantly, the antibiotic resistance to next-generation antibacterial agents (novobiocin and vancomycin) which are currently widely used in medicine should be noted. Gentamicin, rifampicin and vancomycin had the highest efficiency among the tested antibiotics (81.9 %, 86.5 % and 97.4 %, respectively). In order to evaluated the effectiveness of antibiotic treatment strategy, the cows (n = 87) with pre-detected S. aureus were divided into four groups. Gentamicin was administrated in group I (n = 26), erythromycin was administrated in group II (n = 22), rifampicin was administrated in group III (n = 12), and penicillin and amoxicillin were administrated in group IV (n = 27). The animals were considered cured when no S. aureus after the treatment. Rifampicin application was the most effective (91.7 %) whereas gentamicin showed the lowest effectiveness (53.8 %). Moreover, the number of multidrug resistant strains reached 55.6-61.5 % after the treatment comparing to 33.3-43.8 % before t...

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Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

Cited by 14 publications

References 13 publications

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Endolysins as Antimicrobials

ANTIBIOTIC-RESISTANCE PROFILE OF Staphylococcus aureus STRAINS ISOLATED FROM MILK OF HIGH YIELD COWS IN CENTRAL RUSSIA

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