Intramuscular delivery of a cholera DNA vaccine primes both systemic and mucosal protective antibody responses against cholera

Protective antibody responses againstClostridium difficileelicited by a DNA vaccine expressing the enzymatic domain of toxin B

“…Therefore, we have produced codon optimized C. difficile toxin gene sequences that have the potential to achieve high level expression in both mammalian and E. coli systems as previously described. 28 Based on function, toxin A can be divided into four major domains: the N-terminal enzymatic (catalytic) domain, the cysteine protease domain, the translocation domain and the C-terminal receptor binding domain (RBD). 29 Using a codon optimized C. difficile toxin A gene sequence as the template, five toxin A DNA vaccine inserts were produced and cloned into DNA vaccine vector, pJW4303 (Fig.…”

“…While the new codon optimized tcdA and tcdB genes were designed mainly for the development of TcdA and TcdB DNA vaccines, the less optimal codons in the tcdA and tcdB genes were changed to the preferred codons to allow a higher expression of the resulting TcdA and TcdB protein domains in both mammalian and E. coli expression systems. 28 Sequence optimization was also performed to make the mRNA more stable and to make the gene more favorable for transcriptional and translational processes. During sequence optimization, the following cis-acting sequence motifs were avoided: internal TATA-boxes, chi-sites and ribosomal entry sites; AT-rich or GC-rich sequence stretches; INS (inhibitory sequences), CRS (cis-acting repressor sequences) elements; cryptic splice donor and acceptor sites; and branch points.…”

Influenza H5 Hemagglutinin DNA Primes the Antibody Response Elicited by the Live Attenuated Influenza A/Vietnam/1203/2004 Vaccine in Ferrets

“…However, DNA vaccines could serve as a priming agent to significantly increase the immunogenicity of a protein vaccine. Such DNA prime-protein boost approach has been successfully exploited to improve the breadth of the cellular and humoral immune response elicited by various vaccines against different bacterial and protozoan pathogens in animal studies [17], [18], [19], [20], [21], as well as in an HIV vaccine study in humans [22]. Wei et al (2010) recently reported that H1 HA DNA priming followed by a TIV boost not only led to increased neutralizing antibody titers but also broadened the response to antigenically distant H1N1 virus strains [23].…”

Immunization with DNA prime-subunit protein boost strategy based on influenza H9N2 virus conserved matrix protein M1 and its epitope screening

“…Plasmid DNA vaccines as stimulus can effectively enhance the immunogenicity of protein vaccines, so the DNA primeprotein boost strategy can enhance cellular and humoral immune responses. Many animal experiments confirmed that the prime-boost strategy can effectively protect against a variety of bacteria and protozoan pathogens [14][15][16][17][18] . Wei et al used the HA DNA prime-trivalent inactivated vaccine (TIV) boost strategy to induce high titres of neutralization antibodies and cross-reactive immune responses 19 .…”