Intranasal immunization with a recombinant outer membrane protein H based Haemorrhagic septicemia vaccine in dairy calves

Abstract

Haemorrhagic septicemia (HS) is a contagious disease in cattle with high morbidity and mortality rates. HS vaccine in Thailand is an oil-adjuvant formulation, and is difficult to administer. The present study aimed to formulate and evaluate the protection in dairy calves conferred by immunization with an in-house intranasal HS vaccine. The intranasal vaccine was formulated in a total volume of 500 µl containing either 50 or 100 µg of the recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain M-1404 (serovar B:2), and 10 µg of Cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) as a mucosal adjuvant. Intranasal immunizations were conducted three times at three-week intervals. The antibodies post-immunization were detected by indirect ELISA and demonstrated efficient in vitro activity in suppressing a P. multocida strain from the complement-mediated killing assay. An intranasal vaccine induced both the serum IgG and secretory IgA levels that were significantly higher than the level conferred by the parenteral vaccine (P<0.05). Challenge exposure was conducted with a P. multocida strain M-1404 at day 72 of the experiments. The immunized calves had reduced clinical signs after challenge exposure that would normally result in disease proliferation. We conclude that intranasal vaccination of calves with rOmpH with CpG-ODN 2007 stimulated serum and secretory antibodies to rOmpH and whole cells of P. multocida strain M-1404 antigen. Moreover, it would result in protection in calves against artificial P. multocida infection.

The Immunology of Bovine Respiratory Disease

“…Intranasal treatment with P multocida outer membrane protein H induced both serum IgG and secretory IgA levels that protected calves from experimental challenge with P multocida. 49 Although studies have yet to be conducted in cattle, a recombinant outer membrane lipoprotein B from P multocida serotype A strain was shown to induce serum Ab in mice with significant bacterial killing activity. 50 Regarding H somni immunity, antibodies to a 40-kDa outer membrane protein (OMP) have been found protective, whereas those to a 78-kDa OMP are not.…”

An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes

“…Various formulations of HS vaccines are available to treat animals, including inactivated vaccines, live vaccines, purified capsular extract vaccines, and combined vaccines [2]. e parenteral administration of HS is an oiladjuvant formulation, but it is inconvenient for practical use and may induce stress in animals [10]. Although several conventional vaccine formulations are commercially available, the quest for suitable broadly protective HS vaccines with long-lasting immunity is on the upsurge [4].…”

“…Up to date, a variety of modern vaccines, including recombinant vaccines, have been developed as veterinary vaccine candidates for HS prevention [11]. e outer membrane protein H (OmpH) is a surface antigen of P. multocida, recognized as an immunodominant porin and potential target to be a vaccine candidate for pasteurellosis in domestic animals [10,[12][13][14][15]. Concerning the buffaloes and cattle, the 37 kDa OmpH of P. multocida serotype B:2 has been identified and suggested as a highly antigenic protein [16][17][18][19].…”

“…Concerning the buffaloes and cattle, the 37 kDa OmpH of P. multocida serotype B:2 has been identified and suggested as a highly antigenic protein [16][17][18][19]. Also, there was a report that involved the development of a recombinant OmpH (rOmpH) of P. multocida strain M-1404 as an alternative vaccine and demonstrated a sufficient level of protection against HS among vaccinated dairy calves [10].…”

“…Mucosal vaccination via the nasal route is recognized as a noninvasive method of administration and has several advantages over traditional approaches [20]. Since the route of P. multocida infection in buffaloes and cattle is mainly at the upper respiratory tract, the intranasal vaccination would be suitable defense mechanisms against invading pathogens [10,20]. e objective of this study was to formulate an appropriate concentration of rOmpH-based intranasal vaccine and determine the protective capability against P. multocida challenge exposure among buffaloes.…”