Intraocular Irrigating Solutions and Vitrectomy- Related Changes (in Protein, Lactic and Ascorbic Acid) in Rabbit Vitreous

Manzanas, L; Pastor, J. Carlos; Muñoz, Raquel; Girbés, Tomás

doi:10.1159/000267148

Cited by 12 publications

(4 citation statements)

References 16 publications

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“…This might cause harmful retinal oedema, as previously reported by Manzanas et al, or some other type of damage to bipolar, Mueller or other inner retinal cells that contribute to the ERG b-wave. 17 Our limited histological specimens corroborate our electrophysiological findings. This study is not free of some shortcomings: First, analysing b-wave and a-wave ratio and/or recording ERG using the International Society for Clinical Electrophysiology of Vision (ISCEV) standard would have been more informative, as we used total ERG amplitude for the analysis.…”

Section: Discussionsupporting

confidence: 86%

“…2 16 Manzanas et al observed that intraocular concentrations of protein increased to nearly the same extent after vitrectomy in rabbits using BSS-Plus, saline and LR without a statistical difference between each solution. 17 They also observed that lactic acid (assumed to be produced mainly by the retina) increased with saline and LR but not with BSS-Plus. Ascorbic acid was highest in the vitreous if vitrectomy was performed using saline as intraocular irrigating solution.…”

Section: Discussionmentioning

confidence: 96%

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The Effect of Vitrectomy Infusion Solutions on Postoperative Electroretinography and Retina Histology

et al. 2017

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PurposeA prospective, randomised, masked study comparing three different intraocular infusion solutions used during vitrectomy on postoperative electroretinography (ERG) and retinal histology of rabbit eyes.Methods, materials and animalsTwelve rabbit eyes were used with each group of three intraocular infusion solutions: Lactated Ringers (LR), balanced salt solution (BSS) and balanced salt solution-plus (BSS-Plus).ResultsAt 24 hours postoperatively, the ERG values were 36%, 68% and 75% of the normal values for LR, BSS and BSS-Plus, respectively. At 72 hours postoperatively, the ERG values were 50%, 84% and 100% of the normal values for LR, BSS and BSS-Plus, respectively. At 72 hours postoperatively, 3/12, 10/12 and 12/12 of the vitrectomised eyes had regained normal-value ERGs for LR, BSS, and BSS-Plus eyes, respectively. At 1 week postoperatively, 4/12 and 12/12 of LR and BSS eyes, respectively, had normal ERGs. The number of LR eyes regaining normal ERG values was 4/12, 6/12 and 9/12, measured at 1, 4 and 15 weeks postoperatively, respectively.Retina histologyAt 15 weeks follow-up, histopathology was performed on several rabbit eyes. Histological examination of LR-irrigated eyes showed 2/7 normal retinas, whereas 5/7 showed changes that ranged from vacuoles in the inner retina to numerous vacuoles in both the outer and inner retina with disruption of the inner nuclear layer. The histology of BSS-infused and BSS-Plus-infused rabbit eyes was normal.ConclusionLR solution, when used during vitrectomy, has a more prolonged negative effect on ERG than BSS and BSS-Plus. Histological changes seen in LR eyes may explain the ERG results.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

The Effect of Vitrectomy Infusion Solutions on Postoperative Electroretinography and Retina Histology

et al. 2017

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“…In our study, the development of complicated cataract after vitrectomy with silicone oil tamponade occurred in 77% of eyes on day 90 after operation, and it is consistent with the literature mentioned above. It was reported that some proteins, vitamin C, and lactic acid were found elevated in the vitreous cavity after vitrectomy, and all of them could scavenge active free radical and singlet oxygen and convert them into peroxide ions [ 26 ], indicating that the elevation of reactive oxygen species is involved in the process of complicated cataract. Studies have found that the oxidation of lens protein during vitrectomy was one of the reasons for nuclear cataract after vitrectomy [ 27 ], whereas adding antioxidants into the perfusion fluid during vitrectomy could inhibit the abnormal lens epithelial cell growth and lens fiber formation and thus prevent the cataractogenesis [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Tea Polyphenol Ophthalmic Gel on Lens Epithelial Cells in Rabbits with Silicone Oil Tamponade after Vitrectomy

Guo²,

Xie

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Purpose. The aim of this study was to investigate the effect of tea polyphenols (TP) ophthalmic gel on lens epithelial cells (LECs) in rabbits with silicone oil tamponade after vitrectomy. Methods. In this study, unilateral vitrectomy with silicone oil tamponade was performed using 2-month-old New Zealand white rabbits (n = 72); meanwhile, age-matched nonoperated rabbits (n = 18) were used as controls. The TP ophthalmic gel was administered topically in the surgical eyes till they were sacrificed. On days 45 and 90 after operation, the levels of reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm), and apoptosis of LECs were analyzed, respectively. Meanwhile, caspase-3 mRNA and protein levels were also determined. Results. The results indicate that the levels of ROS and apoptosis were elevated for LECs in rabbits after operation, whereas ΔΨm was decreased. Caspase-3 was apparently increased at both mRNA and protein levels. Treatment of TP ophthalmic gel could reduce the generation of ROS, maintain ΔΨm, inhibit the overexpression of caspase-3, and thus decrease the apoptosis of LECs of rabbits after operation. Conclusions. TP ophthalmic gel can efficiently inhibit caspase-3 overexpression, reduce the apoptosis of LECs, and prevent LECs from damage. Our result provides a new approach to prevent the development of complicated cataract after vitrectomy.

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“…After further technical refinements [4,5], vitrectomy has become the second most common surgery in ophthalmology now covering emergency and elective indications [3,6]. During the surgical procedures, many factors that might harm the tissue are among others the use of excessive endoillumination [7], an irrigation solution with a different composition than the human vitreous [8], the suppression of blood flow [9], air infusion [10], drugs [11] and medical devices [12]. All these elements are in direct contact with the retina and can potentially affect the neuronal tissue.…”

Section: Introductionmentioning

confidence: 99%

Retinal Toxicity of Medical Devices Used during Vitreoretinal Surgery: A Critical Overview

et al. 2018

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Retinal toxicity/biocompatibility of medical devices in direct contact with the retina is an important subject for clinicians and scientists. As these effects are not very frequent, there is also a relative lack of information for many clinicians. The past has taught us multiple times that there is a significant safety problem associated with severe loss of vision in affected patients. In this review, we want to classify medical products that are used in the back of the eye, describe recent examples of toxicity, critically reflect on the regulations that exist and suggest improvements that can be done to ensure patient safety without hindering innovation. Methods: Critical review of the recent papers and personal experience of the authors in this issue. Medical devices used in the back of the eye and recent examples of toxicity are described, regulations that exist are critically reflected and improvements suggested that can ensure patient safety without hindering innovation. Results: There is clear evidence of toxicity after intraocular surgery in any category. Some cytotoxic indirect methods have failed in detecting this toxicity. Some ISO rules do not seem appropriate. Postmarketing safety is missing. There is little data on this issue. Conclusions: The absence of a clear regulation of the production, purification and evaluation of the toxic effects of the medical devices supposes the possibility that products are not sufficiently safe to obtain the CE mark.

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Intraocular Irrigating Solutions and Vitrectomy- Related Changes (in Protein, Lactic and Ascorbic Acid) in Rabbit Vitreous

Cited by 12 publications

References 16 publications

The Effect of Vitrectomy Infusion Solutions on Postoperative Electroretinography and Retina Histology

The Effect of Vitrectomy Infusion Solutions on Postoperative Electroretinography and Retina Histology

Protective Effect of Tea Polyphenol Ophthalmic Gel on Lens Epithelial Cells in Rabbits with Silicone Oil Tamponade after Vitrectomy

Retinal Toxicity of Medical Devices Used during Vitreoretinal Surgery: A Critical Overview

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