Intraocular pressure, specular microscopy, and prostaglandinE₂concentration in dogs with mature and hypermature cataract

Abstract

This study aimed to evaluate and correlate intraocular pressure (IOP), endothelial cell density (CD), and hexagonality (HEX), and the aqueous humor prostaglandin E2 (PGE2 ) concentration in dogs with mature (MG, n = 8) and hypermature (HG, n = 8) cataracts. Eight laboratory beagles with no ocular abnormalities were included as a control group (CG). The IOP was measured using a digital applanation tonometer. Noncontact specular microscopy was used to evaluate CD and HEX. Samples of aqueous humor were used to determine prostaglandin E2 concentration using enzyme-linked immunoassay. Data were compared by anova and Bonferroni's multiple comparison test, and possible correlations among the PGE2 aqueous concentration and corneal endothelium cell parameters were assessed by Person's test (P < 0.05). Average values of IOP (P = 0.45) and CD (P = 0.39) were not significantly different between MG, HM, and CG. Average values of HEX were lower, and PGE2 concentration was increased in the MG and HG in comparison with CG (P < 0.05); however, such parameters did not change significantly between MG and HG (P > 0.05). PGE2 values did not correlate with IOP, CD, and HEX in any group (P > 0.05). Although there were a small number of dogs studied, our results demonstrated that cataract progression from mature to hypermature did not have a significant change in PGE2 aqueous concentration, IOP, corneal endothelial cell count, or morphology. In addition, PGE2 concentration was not correlated with parameters of the corneal endothelium or IOP in dogs with mature or hypermature cataracts.

Ophthalmic parameters in adult lowland paca (Cuniculus paca) raised in captivity

“…(2998.0 ± 326 cells/mm 2 and 2518.0 ± 134.9 cells/mm 2 , respectively) in rabbits, it is comparable to those reported by Bercht et al . (2124 cells/mm 2 ) in adult chinchillas and by Renzo et al . (2176.20 ± 432.24 cells/mm²) in dogs.…”

“…The mean IOP (6.34 ± 0.43 mmHg) in the present study was lower than the values established in other wild rodents . Variations in IOP values may arise from intraocular inflammation, glaucoma, variation in blood pressure, senility, seasonality, circadian rhythm, and the effects of certain drugs . In our study, despite the low IOP values, there were no signs of either eye diseases or altered vision.…”

Analysis of the corneal endothelium in eyes of chickens using contact specular microscopy

“…Specular microscopy has become a standard technique to determine endothelial cell density and cell morphology in vivo. The specular microscope has been used to quantify endothelial cell size in humans, dogs, rabbits, horses and other animal species (ABIB; BARRETO, 2001;ANDREW et al, 2001;GWIN et al, 1982;MORITA, 1995;PEIFFER et al, 1981;PIGATTO et al, 2006;RENZO, 2014;STAPLETON;PEIFFER, 1979;SZALAI, 2011). However, most studies do not report the evaluation of the parameters endothelial cells and their relation with aging in birds.…”

“…Used in preoperative evaluation for cataract surgery in humans and animals (RENZO et al, 2014). Among the useful parameters to determine endothelial cell status are the endothelial cell density and cell morphology (SABATER et al, 2013).…”

Concentrations of matrix metalloproteinase-2 and interleukin-1 β in the aqueous humor of dogs with normal and cataractous eyes

“…Cataract is defined the opacity of the eye lens, and is considered to be the leading cause of blindness in dogs. The affection can be classified based on the age of onset (congenital, juvenile, or senile), position in the lens (capsular, cortical, nuclear, axial, equatorial, or total), degree and stage of opacification (incipient, immature, mature, or hypermature), and the possible cause (inherited, traumatic, metabolic, or toxic) (Davidson & Nelms 2013, Renzo et al 2014.…”