Intraperitoneal and intraluminal microdialysis in the detection of experimental regional intestinal ischaemia

Abstract

Regional occlusive ischaemia in 100 cm of small intestine could be detected and monitored by means of a microdialysis catheter placed in the peritoneal cavity or the bowel lumen.

Microdialysis Study of Imipenem Distribution in the Intraperitoneal Fluid of Rats with or without Experimental Peritonitis

“…Microdialysis is a reliable technique for investigating the kinetics of antibiotics in different tissues, and it has been used on many occasions in various tissues, such muscle, lung, brain, and skin, in both animals and humans (18). However, no microdialysis antibiotic-distribution study has ever been conducted in intraperitoneal fluid to our knowledge, although the technique has been used to monitor intraperitoneal concentrations of markers of ischemia, such as lactate, mainly in pigs (13,22,24), but also in humans (8,19) and rats (2,10,11,12).…”

Diagnostic value of plasminogen activity level in acute mesenteric ischemia

“…A significant rise in serum phosphate level occurred after 4 h of ischemia, which was not helpful for an early diagnosis [19] . Regional intestinal ischemia is reported to be detected and monitored by means of a microdialysis catheter placed in the peritoneal cavity or the bowel lumen by measuring lactate, pyruvate, glycerol and glucose levels [21] .…”

Microdialysis Monitoring of Biomarkers for Early Recognition of Intestinal Ischemia

“…However, intraluminal metabolites measurements have been found to be extremely low and the detection is not feasible due to the resting phase of the intestine and to the high volume of contents in the lumen (126). A common approach is to place the microdialysis probe in the human peritoneal cavity (127)(128)(129).…”

“…However, the ischemic biomarkers are diluted by non-ischemic markers and by the systemic circulation supplied to the peritoneum (78). Sommer et al compared results from microdialysis probes inserted intraperitoneally, intramurally and intraluminally in the bowel of a swine model (126) (Figure 6). Insertion of the probe intramurally provides faster detection of metabolic changes than intraluminal and peritoneal microdialysis due to the proximity of the probe to the damaged tissue and the lack of dilution artifacts (130).…”