Intrapulmonary perfluorooctyl bromide instillation in fetal rabbits

Intrapulmonary Instillation of Perflurooctylbromide Improves Lung Growth, Alveolarization, and Lung Mechanics in a Fetal Rabbit Model of Diaphragmatic Hernia

“…The use of PFOB for fetal lung distension in case of lung hypoplasia in the human fetus might be feasible. However, it has been detected in the fetal brain after intratracheal application in the rabbit fetus (19). The brain tissue concentration was comparable to blood levels determined during postnatal partial liquid ventilation of premature lambs (19,50).…”

“…However, it has been detected in the fetal brain after intratracheal application in the rabbit fetus (19). The brain tissue concentration was comparable to blood levels determined during postnatal partial liquid ventilation of premature lambs (19,50). In vitro studies on root ganglion cells have shown an alteration of neuronal cell populations after brief exposure to PFOB (51).…”

“…The feasibility of prenatal instillation of PFOB into the lungs of fetal rabbits has previously been studied (18)(19)(20). When instilled into healthy fetal rabbit lungs, PFOB increased lung growth without a reduction of surfactant protein B-positive cells as compared to TO (18,20).…”

Postnatal lung mechanics, lung composition, and surfactant synthesis after tracheal occlusion vs prenatal intrapulmonary instillation of perfluorocarbon in fetal rabbits

“…Perfluorooctylbromide acts as a temporary TO; after intratracheal instillation it remains in the airways, although in small quantities, for at least 48 hours [16]. Therefore, it will cause lung stretch with attenuated tissue distension compared with complete and permanent TO.…”

Temporary fetal tracheal occlusion using a gel plug in a rabbit model of congenital diaphragmatic hernia

“…Previously, the concept of filling the prenatal lungs with PFC was developed [12], with the intention of using the static pressure of this dense liquid on the alveoli to induce alveolar stretch and thereby accelerate lung growth. This concept had some success in a rabbit model but also came with the drawback that the injected PFC quickly dissipated because of its low viscosity, shortening the effect [13].…”