Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of<i>Pythium mercuriale</i>sp. nov. (<i>Pythiaceae</i>)

Belbahri, Lassaâd; McLeod, A.; Paul, Bernard; Calmin, Gautier; Moralejo, Eduardo; Spies, Chris; Botha, W. J.; Clemente, Antonio; Descals, Enrique; Sánchez-Hernández, M. E.; Lefort, François

doi:10.1111/j.1574-6968.2008.01168.x

Cited by 55 publications

(36 citation statements)

References 35 publications

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“…The ITS sequences of the isolate V61 showed high sequence identity (99.4%) with the ex-type isolate STE-U 6204 of Py. mercuriale (Belbahri et al, 2008). The sequence of the isolate V80 also showed high sequence identity (96.3%) with the extype isolate CBS286.31 of Py.…”

Section: Identification Of Pythium and Phytophthora Isolatesmentioning

confidence: 79%

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Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Kato

Minamida

Tojo

et al. 2013

Plant Production Science

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In Japan, soybean is usually cultivated in fields that have been converted from rice paddies, and poor seedling establishment due to pre-emergence seedling damping-off is often observed during the rainy season. In this study, the factors that cause the damping-off in flooded soil were investigated under high soil moisture conditions in a greenhouse and in agricultural fields. In sterilized soil sampled from a soybean field, seedlings emerged well under 48-hr flooded conditions. In unsterilized soil, soybean seeds treated with the fungicide, mancozeb +metalaxyl exhibited much higher emergence rates even under 48-hr flooded conditions than the seeds treated with oxytetracycline +streptomycin, benomyl, or flutolanil. Pythium, Phytophthora, Mucorales, Trichoderma, Geotrichum-like microorganisms, and some fungi producing conidia in a false head, were isolated from decayed seedlings. Of the isolated microorganisms, oomycete microorganisms, Pythium helicoides, other Pythium sp., and Phytophthora sp. were pathogenic to soybeans under flooded conditions. As the length of the flooding period increased, pre-emergence seedling rot also increased. However, the pathogenic oomycetes rarely caused pre-emergence seedling rot under conditions without flooding. Furthermore, under flooded conditions, the damage caused by these pathogens was reduced by treating the seeds with mancozeb+metalaxyl. These results indicate that oomycete microorganisms are involved in pre-emergence seedling damping-off under flooded soil conditions.

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Section: Identification Of Pythium and Phytophthora Isolatesmentioning

confidence: 79%

“…Pythium isolates V61 and V80 only produced asexual structures that corresponded with those of Py. mercuriale (Belbahri et al, 2008) and Py. helicoides (van der Plaats-Niterink, 1981), respectively.…”

Section: Identification Of Pythium and Phytophthora Isolatesmentioning

confidence: 99%

Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Kato

Minamida

Tojo

et al. 2013

Plant Production Science

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show abstract

“…isolated in this water survey, Pythium amasculinum, Pythium mercuriale, Pythium adhaerens, and Pythium oedochilum, have not been previously reported from surface water sources. Each Pythium species found in this survey has previously been isolated from a diseased crop plant (38)(39)(40).…”

Section: Discussionmentioning

confidence: 99%

Plant-Pathogenic Oomycetes, Escherichia coli Strains, and Salmonella spp. Frequently Found in Surface Water Used for Irrigation of Fruit and Vegetable Crops in New York State

Jones

Worobo

Smart

2014

Appl Environ Microbiol

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bIn the United States, surface water is commonly used to irrigate a variety of produce crops and can harbor pathogens responsible for food-borne illnesses and plant diseases. Understanding when pathogens infest water sources is valuable information for produce growers to improve the food safety and production of these crops. In this study, prevalence data along with regression tree analyses were used to correlate water quality parameters (pH, temperature, turbidity), irrigation site properties (source, the presence of livestock or fowl nearby), and precipitation data to the presence and concentrations of Escherichia coli, Salmonella spp., and hymexazol-insensitive (HIS) oomycetes (Phytophthora and Pythium spp.) in New York State surface waters. A total of 123 samples from 18 sites across New York State were tested for E. coli and Salmonella spp., of which 33% and 43% were positive, respectively. Additionally, 210 samples from 38 sites were tested for HIS oomycetes, and 88% were found to be positive, with 10 species of Phytophthora and 11 species of Pythium being identified from the samples. Regression analysis found no strong correlations between water quality parameters, site factors, or precipitation to the presence or concentration of E. coli in irrigation sources. For Salmonella, precipitation (<0.64 cm) 3 days before sampling was correlated to both presence and the highest counts. Analyses for oomycetes found creeks to have higher average counts than ponds, and higher turbidity levels were associated with higher oomycete counts. Overall, information gathered from this study can be used to better understand the food safety and plant pathogen risks of using surface water for irrigation.

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“…21. The β-tubulin region has also been used to a limited extent in Pythium phylogenies (Villa et al 2006;Belbahri et al 2008;Spies et al 2011a, b). Although this region fails to resolve Pythium into the genera observed when using the dataset from Fig.…”

Section: Recommended Genetic Markersmentioning

confidence: 99%

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

et al. 2014

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Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry. They are also part of the natural ecosystem and may play a role in regulating plant numbers/density. Morphological identification and analysis of plant pathogenic fungi, while important, is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi. Molecular (DNA sequence) data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies, although hampered in part by non-standard laboratory practices and analytical methods. To facilitate current and future research, this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota, Basidiomycota, Mucormycotina (Fungi), and Oomycota, using recent molecular data, up-to-date names, and the latest taxonomic insights. Lineagespecific laboratory protocols together with advice on their application, as well as general observations, are also provided. We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge. Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort. Bipolaris, Botryosphaeriaceae,

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Intraspecific and within-isolate sequence variation in the ITS rRNA gene region ofPythium mercurialesp. nov. (Pythiaceae)

Cited by 55 publications

References 35 publications

Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Association of Pythium and Phytophthora with Pre-emergence Seedling Damping-off of Soybean Grown in a FieldConverted from a Paddy Field in Japan

Plant-Pathogenic Oomycetes, Escherichia coli Strains, and Salmonella spp. Frequently Found in Surface Water Used for Irrigation of Fruit and Vegetable Crops in New York State

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

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