Intraspecific mitochondrial DNA variation in the Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Azeredo-Espin, Ana Maria Lima de; Schröder, Robert; Roderick, George; Sheppard, Walter S.

doi:10.1007/bf02399946

Cited by 15 publications

(10 citation statements)

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“…Restriction endonuclease digestion of mitochondrial DNA revealed the presence of 16 haplotypes of this beetle occurring in the USA (Azeredo-Espin et al 1996). An earlier study done using the same approach on 16 populations from the USA and one from Mexico showed five distinct mtDNA genotypes among the U.S. populations and high divergence between these genotypes and the genotype of the Mexican population.…”

Section: Resultsmentioning

confidence: 99%

High Stability of a Mitochondrial Genetic Marker mtCOII in Polish Colorado Potato Beetle Populations

et al. 2014

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Colorado potato beetle (CPB) (Leptinotarsa decemlineata (Say in Journal of the Academy of Natural Sciences of Philadelphia 3: 298-331, 1824)) (Coleoptera: Chrysomelidae) is one of the most serious potato pests. It has been reported worldwide, from North America to Europe and Asia. In this study we analyzed the genetic diversity of a mitochondrial DNA marker -a second subunit of cytochrome oxidase (mtCOII) in Polish CPB populations to assess the possible changes of this gene sequence over time and over the country, influencing the intra-specific variability of CPB. During a three-year survey in Polish potato fields the beetles were collected from 20 evenly spaced locations of varying climatic and geographic conditions, and the nucleotide sequence of this marker was analyzed. Our research revealed that in spite of three years of sampling the mitochondrial haplotype in all individuals was fixed, and no single nucleotide change was found in any individual, indicating a high stability of this maternally inherited marker in L. decemlineata. This finding about the level of biodiversity is of importance for plant protection strategies.Resumen El escarabajo de la papa de Colorado (CPB) (Leptinotarsa decemlineata (Say in Journal of the Academy of Natural Sciences of Philadelphia 3: 298-331, 1824)) (Coleoptera: Chrysomelidae) es una de las plagas más serias en papa. Se ha reportado en todo el mundo, desde Norteamérica a Europa y Asia. En este estudio analizamos la diversidad genética de un marcador de ADN mitocondrial -una segunda subunidad de citocromo oxidasa (mtCOII) en poblaciones polacas de CPB para ver los posibles cambios en la secuencia de este gene en el tiempo y en el país, que estuvieran influenciando la variabilidad intraespecífica del CPB. Durante los tres años del estudio en los campos de papa polacos se colectaron los escarabajos de veinte localidades espaciadas uniformemente de condiciones climáticas y geográficas variables, analizando la secuencia de nucleótidos de este marcador. Nuestra investigación reveló que a pesar de los muestreos por tres años, el haplotipo mitocondrial era fijo en todos los individuos, y no se encontró un solo cambio de nucleótido en ningún individuo, indicando una gran estabilidad de este marcador heredado maternalmente en L. decemlineata.

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Section: Resultsmentioning

confidence: 99%

High Stability of a Mitochondrial Genetic Marker mtCOII in Polish Colorado Potato Beetle Populations

et al. 2014

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“…As a final possibility, there is some evidence that potato beetles in Washington appear to be less genetically diverse than beetles occurring elsewhere . Indeed, this unique, low‐diversity genetic make‐up may make a key contribution to the lack of insecticide resistance in Washington potato beetles.…”

Section: Getting Off the Treadmillmentioning

confidence: 86%

“…102 -104 Indeed, this unique, low-diversity genetic make-up may make a key contribution to the lack of insecticide resistance in Washington potato beetles. For example, Azeredo-Espin et al, 104 using restriction endonuclease analysis of mitochondrial DNA, found that potato beetles in Washington all fell into a single haplotype that was not found in any of the other nine geographically diverse populations that they examined (see the similar finding by Zehnder et al 102 ). Similarly, a widely dispersed haplotype found in each of the other nine populations was not found in Washington.…”

Section: Washington State -A Place Where Old Chemistries Still Workmentioning

confidence: 87%

“…For example, Azeredo‐Espin et al , using restriction endonuclease analysis of mitochondrial DNA, found that potato beetles in Washington all fell into a single haplotype that was not found in any of the other nine geographically diverse populations that they examined (see the similar finding by Zehnder et al ). Similarly, a widely dispersed haplotype found in each of the other nine populations was not found in Washington . It is possible that the beetles in Washington went through an atypically severe genetic bottleneck during initial colonization of potatoes.…”

Section: Getting Off the Treadmillmentioning

confidence: 99%

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The Red Queen in a potato field: integrated pest management versus chemical dependency in Colorado potato beetle control

et al. 2014

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Originally designed to reconcile insecticide applications with biological control, the concept of integrated pest management (IPM) developed into the systems-based judicious and coordinated use of multiple control techniques aimed at reducing pest damage to economically tolerable levels. Chemical control, with scheduled treatments, was the starting point for most management systems in the 1950s. Although chemical control is philosophically compatible with IPM practices as a whole, reduction in pesticide use has been historically one of the main goals of IPM practitioners. In the absence of IPM, excessive reliance on pesticides has led to repeated control failures due to the evolution of resistance by pest populations. This creates the need for constant replacement of failed chemicals with new compounds, known as the 'insecticide treadmill'. In evolutionary biology, a similar phenomenon is known as the Red Queen principle - continuing change is needed for a population to persevere because its competitors undergo constant evolutionary adaptation. The Colorado potato beetle, Leptinotarsa decemlineata (Say), is an insect defoliator of potatoes that is notorious for its ability to develop insecticide resistance. In the present article, a review is given of four case studies from across the United States to demonstrate the importance of using IPM for sustainable management of a highly adaptable insect pest. Excessive reliance on often indiscriminate insecticide applications and inadequate use of alternative control methods, such as crop rotation, appear to expedite evolution of insecticide resistance in its populations. Resistance to IPM would involve synchronized adaptations to multiple unfavorable factors, requiring statistically unlikely genetic changes. Therefore, integrating different techniques is likely to reduce the need for constant replacement of failed chemicals with new ones.

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“…To develop appropriate and effective integrated control strategies, CPB spatial distribution and migration must be well understood (Boiteau et al, 2003). Mitochondrial and nuclear DNA, had provided valuable information on colonization origins, the extent of genetic differentiation, and gene flow during the expansion of CPB in North America and Europe (Zehnder et al, 1992;Azeredo-Espin et al, 1996;Sidorenko & Berezovska, 2002;Grapputo et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships of introduced Colorado potato beetle Leptinotarsa decemlineata populations in Xinjiang, China

Zhang¹,

Yang²,

Li³

et al. 2012

Insect Science

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The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is an infamous invasive species worldwide that aggressively attacks potato and other Solanaceae crops. CPB was first found in China in 1993 and has since spread across 2.77 × 10 5 km 2 in Xinjiang Uygur Autonomous Region. To better understand genetic variation and migration patterns, we used seven polymorphic microsatellite loci to elucidate the genetic relationships and gene flow among 10 CPB populations across Xinjiang. (i) Overall low levels of genetic diversity were detected on the entire population in Xinjiang but most of the diversity was retained among populations during invasion.(ii) The mean pairwise F ST was low (0.071 ± 0.043) among populations. The genetic differentiation was little (pairwise F ST 0.038 ± 0.016) between the five interior populations (Wusu, Urumqi, Jimsar, Qitai and Mulei) and Tacheng population. The six populations might come from the same genetic group via Bayesian clustering and were closely related on a neighbor-joining tree. Combining the history data, the five interior populations may have originated from Tacheng.(iii) Gene flow was frequent, especially among the five interior populations. Individuals from the interior populations could be assigned to Tacheng at higher probabilities (means 0.518 ± 0.127) than vice versa (means 0.328 ± 0.074), suggesting that the beetle population has spread from the border to the interior in Xinjiang.

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Intraspecific mitochondrial DNA variation in the Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Cited by 15 publications

References 42 publications

High Stability of a Mitochondrial Genetic Marker mtCOII in Polish Colorado Potato Beetle Populations

High Stability of a Mitochondrial Genetic Marker mtCOII in Polish Colorado Potato Beetle Populations

The Red Queen in a potato field: integrated pest management versus chemical dependency in Colorado potato beetle control

Genetic relationships of introduced Colorado potato beetle Leptinotarsa decemlineata populations in Xinjiang, China

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