Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates

Marques, Lucas Miranda; Buzinhani, Melissa; Guimarães, Ana M. S.; Marques, Regina Célia Pereira; Farías, Sávio Torres de; Neto, R. L.; Yamaguti, Maurício; Oliveira, Renata Cristina Gobbi de; Timenetsky, Jorge

doi:10.1016/j.vetmic.2011.04.007

Cited by 13 publications

(12 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, inactivated and, especially, viable U. diversum (strains ATCC 49782 and CI-GOTA) organisms Up-Regulated gene expression of IL-1β, TNF-α, TLR2, and TLR4 in bovine macrophages. The expression of this proinflammatory gene profile induced by U. diversum in macrophages is supportive evidence of the severe inflammation found in the reproductive tract of infected cows ( Cardoso et al, 2000a , b ; Buzinhani et al, 2007a ; Marques et al, 2009 , 2011 ; Maunsell and Donovan, 2009 ). Murine macrophages inoculated with viable or inactivated U. diversum have also shown increased TLR2 gene expression and production of IL-1β and TNF-α ( Marques et al, 2016 ).…”

Section: Discussionsupporting

confidence: 62%

“…The pathogenicity of U. diversum is not adequately understood but the activation of macrophages through TLRs has been frequently mentioned as an important feature in mycoplasmosis ( Shimizu et al, 2008a ; Majumder et al, 2014 ; Wang et al, 2016 ). U. diversum is also associated with reproductive disorders in bovine, as it causes severe inflammation, cell invasion and modulates apoptosis ( Cardoso et al, 2000b ; Buzinhani et al, 2007b ; Marques et al, 2007 , 2009 , 2010 , 2011 ; Hobson et al, 2013 ; Amorim et al, 2014 ). In the present study, inactivated and, especially, viable U. diversum (strains ATCC 49782 and CI-GOTA) organisms Up-Regulated gene expression of IL-1β, TNF-α, TLR2, and TLR4 in bovine macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…Ureaplasma diversum has been reported to infect the respiratory and genital tracts of cattle and cause a myriad of reproductive alterations, including granular vulvovaginitis, endometritis, salpingitis, infertility, abortion, and changes in spermatozoa morphology ( Marques et al, 2011 ; Hobson et al, 2013 ). Despite these reports, studies on the relationship of U. diversum and bovine reproductive disorders have been regarded controversial mainly due its finding in healthy animals ( Taylor-Robinson et al, 1967 ; Howard et al, 1973 ; Ball and Mackie, 1985 ; Buzinhani et al, 2007b ; Marques et al, 2011 ). Nevertheless, the process that leads to infertility or pregnancy failures in U. diversum infected cows is believed to occur due to the pathogen’s damage to the oocyte, uterus and epithelium of the oviduct, which may interfere with the embryo development and possibly lead to embryonic death ( Doig et al, 1980 ; Britton et al, 1987 ; Lewandowska-Sabat et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

“…The immunomodulatory effects of U. diversum infection in blastocysts, however, have never been evaluated. In addition, U. diversum has been recently shown to invade Hep-2 cells and bovine spermatozoids in vitro ( Marques et al, 2010 , 2011 ; Hobson et al, 2013 ). It is likely that this intracellular activity hampers antibiotic action and the host immune response against U. diversum in infected animals ( Amorim et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

et al. 2018

View full text Add to dashboard Cite

Objectives: Ureaplasma diversum is a pathogen of cows that may cause intense inflammatory responses in the reproductive tract and interfere with bovine reproduction. The aims of this study were to evaluate the immune response of bovine blastocysts and macrophages to U. diversum infection and to evaluate the invasion capacity of this microorganism in bovine blastocysts.Methods: Viable and heat-inactivated U. diversum strains ATCC 49782 and CI-GOTA and their extracted membrane lipoproteins were inoculated in macrophages in the presence or absence of signaling blockers of Toll-Like Receptor (TLR) 4, TLR2/4, and Nuclear Factor KB (NF-κB). In addition, the same viable U. diversum strains were used to infect bovine blastocysts. RNA was extracted from infected and lipoprotein-exposed macrophages and infected blastocysts and assayed by qPCR to evaluate the expression of Interleukin 1 beta (IL-1β), Tumor Necrosis Factor Alpha (TNF-α), TLR2 and TLR4 genes. U. diversum internalization in blastocysts was followed by confocal microscopy.Results: Both Ureaplasma strains and different concentrations of extracted lipoproteins induced a higher gene expression of IL-1β, TNF-α, TLR2, and TLR4 in macrophages (p < 0.05) when compared to non-infected cells. The used blockers inhibited the expression of IL-1β and TNF-α in all treatments. Moreover, U. diversum was able to internalize within blastocysts and induce a higher gene expression of IL-1b and TNF- α when compared to non-infected blastocysts (p < 0.05).Conclusion: The obtained results strongly suggest that U. diversum and its lipoproteins interact with TLR4 in a signaling pathway acting via NF-kB signaling to stimulate the inflammatory response. This is the first study to evaluate the in vitro immunological response of macrophages and bovine blastocysts against U. diversum. These results may contribute to a better understanding of the immunomodulatory activity and pathogenicity of this infectious agent.

show abstract

Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

et al. 2018

View full text Add to dashboard Cite

show abstract

“…However, despite the description of these possible causal associations, the relationship of U . diversum and reproductive disorders in bovine remains controversial, mainly because high rates of positive vaginal cultures were also detected in animals with normal reproductive rates [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Ureaplasma diversum Genome Provides New Insights about the Interaction of the Surface Molecules of This Bacterium with the Host

et al. 2016

View full text Add to dashboard Cite

Whole genome sequencing and analyses of Ureaplasma diversum ATCC 49782 was undertaken as a step towards understanding U. diversum biology and pathogenicity. The complete genome showed 973,501 bp in a single circular chromosome, with 28.2% of G+C content. A total of 782 coding DNA sequences (CDSs), and 6 rRNA and 32 tRNA genes were predicted and annotated. The metabolic pathways are identical to other human ureaplasmas, including the production of ATP via hydrolysis of the urea. Genes related to pathogenicity, such as urease, phospholipase, hemolysin, and a Mycoplasma Ig binding protein (MIB)—Mycoplasma Ig protease (MIP) system were identified. More interestingly, a large number of genes (n = 40) encoding surface molecules were annotated in the genome (lipoproteins, multiple-banded antigen like protein, membrane nuclease lipoprotein and variable surface antigens lipoprotein). In addition, a gene encoding glycosyltransferase was also found. This enzyme has been associated with the production of capsule in mycoplasmas and ureaplasma. We then sought to detect the presence of a capsule in this organism. A polysaccharide capsule from 11 to 17 nm of U. diversum was observed trough electron microscopy and using specific dyes. This structure contained arabinose, xylose, mannose, galactose and glucose. In order to understand the inflammatory response against these surface molecules, we evaluated the response of murine macrophages J774 against viable and non-viable U. diversum. As with viable bacteria, non-viable bacteria were capable of promoting a significant inflammatory response by activation of Toll like receptor 2 (TLR2), indicating that surface molecules are important for the activation of inflammatory response. Furthermore, a cascade of genes related to the inflammasome pathway of macrophages was also up-regulated during infection with viable organisms when compared to non-infected cells. In conclusion, U. diversum has a typical ureaplasma genome and metabolism, and its surface molecules, including the identified capsular material, represent major components of the organism immunopathogenesis.

show abstract

Ureaplasma

Dando¹,

Sweeney²,

Knox³

2019

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

U.re.a.plas'ma. N.L. fem. n. urea urea; Gr. neut. n. plasma anything formed or molded, image, figure; N.L. neut. n. Ureaplasma urea form. Tenericutes / Mollicutes / Mycoplasmatales / Mycoplasmataceae / Ureaplasma Bacteria in the genus Ureaplasma , pleomorphic, small (100–800 nm in diameter), primarily coccoid cells, devoid of a cell wall, surrounded by a trilaminar‐membrane structure. Coccobacillary and filamentous forms also occur. These bacteria form exceptionally tiny (<0.2 mm) colonies on solid media. Similar to the closely related genus Mycoplasma , ureaplasmas have small (0.75–1.01 Mb) A+T‐rich genomes, use the codon UGA to encode tryptophan, and are nutritionally fastidious. Unlike mycoplasmas they hydrolyze urea rather than glucose or arginine for ATP synthesis. DNA–DNA hybridizations, serotyping of phase‐variable surface‐exposed lipoproteins [the multiple‐banded antigen (MBA)], PCR, and sequencing of the multiple‐banded antigen gene ( mba ), 16S rRNA, and urease genes have defined the species and serovars for this genus. In nature, all species are commensals and/or opportunistic pathogens of vertebrate hosts. Based on the most recent comparative genome analyses, a consensus is emerging that strains associated with humans, the species Ureaplasma urealyticum and Ureaplasma parvum , undergo extensive horizontal gene transfer and may exist collectively as quasi‐species rather than as stable serovars in their native environment. DNA G + C content (mol%) : 25.4–31.9 (complete genome sequences). Type species : Ureaplasma urealyticum Shepard et al. 1974 AL emend. Robertson et al. 2002.

show abstract

Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates

Cited by 13 publications

References 29 publications

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

Ureaplasma diversum and Its Membrane-Associated Lipoproteins Activate Inflammatory Genes Through the NF-κB Pathway via Toll-Like Receptor 4

Ureaplasma diversum Genome Provides New Insights about the Interaction of the Surface Molecules of This Bacterium with the Host

Ureaplasma

Contact Info

Product

Resources

About