Intrathecal granulocyte colony-stimulating factor modulate glial cell line-derived neurotrophic factor and vascular endothelial growth factor A expression in glial cells after experimental spinal cord ischemia

Chen, C.-H.; Huang, Sheng-Lung; Chen, N.-F.; Feng, Chien-Wei; Hung, Han-Chun; Sung, Chun Sung; Jean, Yen-Hsuan; Wen, Zhi Hong; Chen, W.-F.

doi:10.1016/j.neuroscience.2013.02.017

Cited by 25 publications

(19 citation statements)

References 124 publications

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“…For the histopathological examination, the rats were sacrificed after perfusion with PBS and 4% paraformaldehyde as described previously [29, 30], and the skin was removed and fixed in 4% paraformaldehyde for 3 days. The specimens were dehydrated in a graded series of alcohol by rotary tissue processor (Sakura Finetek, Japan) and embedded with paraffin, and 2-μm sections were prepared for hematoxylin and eosin staining to assess the general and pathological changes.…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

Zhicheng

et al. 2016

BMC Complement Altern Med

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BackgroundThe shell of Haliotis diversicolor, or shijueming (SJM), is a type of traditional Chinese medicine. The SJM has appeared in historical records as early as the third and fourth centuries. Historical records have revealed that SJM had mainly been used to treat eye diseases. After the Qing Dynasty (1757), records had emerged, detailing the use of SJM for treating skin injuries, particularly for treating poorly managed ulcers or traumatic wounds. Furthermore, in our anti-inflammation-screening system, SJM significantly inhibited the expression of pro-inflammatory proteins. Previous studies have yet to adopt an animal model to verify the phenomenon and described in the historical records regarding the efficacy of SJM in promoting wound healing. Besides, the mechanism of wound healing effect of SJM is also not clear.MethodsThis study applied in vitro and in vivo models, tissue section analysis, and western blotting to evaluate the effect of SJM on wound healing. The RAW 264.7 cells were used in anti-inflammatory activity assay and phagocytic assay. Male Wistar rats were used to evaluate the effect of SJM on burn injury healing. A copper block (2 × 2 cm, 150 g) preheated to 165 °C in a dry bath was used to contact the skin area for 10 s, thus creating a full-thickness burn injury. The results were analyzed by hematoxylin and eosin staining, picrosirius red staining and Western blotting.ResultsThe results revealed that in the in vitro model, the presence of SJM decreased the inducible nitric oxide synthase (iNOS) expression and enhanced the functions of macrophages. The results of the rat burn injury model revealed that SJM decreased neutrophil infiltration, promoted wound healing, thus increasing the collagen I content and promoting the expression of transforming growth factor-beta 1 (TGF-β1) protein. We speculate that the effect and mechanism of SJM on promoting wound healing is related to macrophage activation. In the inflammation phase, SJM alleviates inflammation by inhibiting iNOS expression and removing neutrophils through phagocytosis. Furthermore, SJM induces the secretion of TGF-β1, converting collagen during the tissue remodeling phase.ConclusionsAccording to our review of relevant literature, this is the first study that applied an evidence-based method to verify that SJM alleviates inflammation, enhances phagocytosis, and triggers wound healing after burn injury. The study findings reveal that SJM provides a promising therapeutic option for treating burn injury.

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Section: Methodsmentioning

confidence: 99%

Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

Zhicheng

et al. 2016

BMC Complement Altern Med

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“…Female Wistar rats (weight, 300–320 g) were used in the experiments. As described in a previous study , i.t. catheters were inserted via the atlantooccipital membrane into the i.t.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous study indicated that i.t. G‐CSF can modulate spinal cord ischemia‐induced glial cell (astrocyte and microglia) activation and upregulate growth factors expression in glial cells . Kawabe et al.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Effects of Direct Intrathecal Administration of Granulocyte Colony‐Stimulating Factor in Rats with Spinal Cord Injury

Chen

et al. 2015

CNS Neurosci Ther

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“…G-CSF upregulates chaperone proteins, such as nucleophosmin-1 in motoneurons after spinal cord hemisection [83], reduces myeloperoxidase activity and lipid peroxidation [84] and increases glial cell line-derived neurotrophic factor and vascular endothelial growth factor A expression in glial cells after spinal cord ischemia [85]. It also appears that G-CSF-associated neuroprotection, through its mobilization capacity, is equivalent to bone marrow mononuclear stem cell-induced neuroprotection [86].…”

Section: Granulocyte Colony-stimulating Factor: Its Implication In Exmentioning

confidence: 99%

Neutrophil contribution to spinal cord injury and repair

Neirinckx

Coste

Franzen

et al. 2014

J Neuroinflammation

124

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Spinal cord injuries remain a critical issue in experimental and clinical research nowadays, and it is now well accepted that the immune response and subsequent inflammatory reactions are of significant importance in regulating the damage/repair balance after injury. The role of macrophages in such nervous system lesions now becomes clearer and their contribution in the wound healing process has been largely described in the last few years. Conversely, the contribution of neutrophils has traditionally been considered as detrimental and unfavorable to proper tissue regeneration, even if there are very few studies available on their precise impact in spinal cord lesions. Indeed, recent data show that neutrophils are required for promoting functional recovery after spinal cord trauma. In this review, we gathered recent evidence concerning the role of neutrophils in spinal cord injuries but also in some other neurological diseases, highlighting the need for further understanding the different mechanisms involved in spinal cord injury and repair.Electronic supplementary materialThe online version of this article (doi:10.1186/s12974-014-0150-2) contains supplementary material, which is available to authorized users.

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Intrathecal granulocyte colony-stimulating factor modulate glial cell line-derived neurotrophic factor and vascular endothelial growth factor A expression in glial cells after experimental spinal cord ischemia

Cited by 25 publications

References 124 publications

Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

Neuroprotective Effects of Direct Intrathecal Administration of Granulocyte Colony‐Stimulating Factor in Rats with Spinal Cord Injury

Neutrophil contribution to spinal cord injury and repair

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