Intravenous cell therapy for acute renal failure with serum amyloid A protein-reprogrammed cells

Abstract

JH. Intravenous cell therapy for acute renal failure with serum amyloid A proteinreprogrammed cells.

Garlic supplemented diet attenuates gentamicin nephrotoxicity ın rats

“…However, their clinical use is limited by their side effects, and especially their high nephrotoxicity by reactive oxygen species and oxidative stress (1). Gentamicin nephrotoxicity has been reproduced in various experimental animal models (mice, rat and rabbit) (13)(14)(15), and numerous agents (1,16,17) have been studied in order to investigate their ability to reduce this nephrotoxicity. As reported by Badreldin HA et al, medicinal plants extracts, compounds from aged garlic, withania somnifera, sesame oil (sesamum indicum, antioxidant vitamins C and E), nitric oxide, and dihydropyridine calcium channel blockers are some of the agents that have been studied (18).…”

Intravenous renal cell transplantation with SAA1-positive cells prevents the progression of chronic renal failure in rats with ischemic-diabetic nephropathy

“…IRCT was performed with normal adult male ZS rat renal tubular cells reprogrammed to express a murine isoform of SAA1 (15) before transplantation. These reprogrammed primary renal cells grow out of harvested renal tubules transfected with the SAA gene and, after 1 wk, form de novo tubules in vitro, the basic structure of renal epithelial redifferentiation and a presumed requirement for their regenerative supporting effects (15,16). SAA expression was chosen to reprogram and redifferentiate cultured renal cells because of its strong tubulogenic property (15), an idea supported by our current and previous work (15,16,19).…”

“…These reprogrammed primary renal cells grow out of harvested renal tubules transfected with the SAA gene and, after 1 wk, form de novo tubules in vitro, the basic structure of renal epithelial redifferentiation and a presumed requirement for their regenerative supporting effects (15,16). SAA expression was chosen to reprogram and redifferentiate cultured renal cells because of its strong tubulogenic property (15), an idea supported by our current and previous work (15,16,19). For example, we found that even poorly differentiated rat NRK52E cells dramatically improved renal function in multiple models of acute kidney injury only when these cells expressed SAA (16).…”

“…Immunohistochemistry for proliferating cell nuclear antigen (PCNA) was performed using anti-PCNA (sc-7907, Santa Cruz Biotechnology, Santa Cruz, CA) and Texas red secondary antibody. Quantification of PCNA-positive and apoptotic cells [determined by characteristic nuclear morphology of 4=,6-diamidino-2-phenylindole (DAPI)-labeled nuclei (14,16)] was performed on blinded sections. Kidneys fixed in 4% paraformaldehyde were also sectioned into 100-m slices with a Vibratome (Vibratome, St. Louis, MO).…”

“…Kidneys fixed in 4% paraformaldehyde were also sectioned into 100-m slices with a Vibratome (Vibratome, St. Louis, MO). Sections were immersed in PBS with 0.2% Triton X-100 for 5 min, washed three times with PBS, blocked for 15 min in PBS with 0.2% BSA, incubated with the rabbit primary anti-SAA antibody (16,19) in PBS for 30 min at 37°C, and then washed in PBS. Fluorescent secondary antibody, Texas redconjugated donkey anti-rabbit (catalog no.…”