Introducing sorghum <i>DREB2</i> gene in maize (<i>Zea mays</i> L.) to improve drought and salinity tolerance

Izadi-Darbandi, Ali; Alameldin, Hussien F.; Namjoo, Nima; Ahmad, Khalil

doi:10.1002/bab.2458

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…In this study, the regulatory role of CmoDREB2A in salt tolerance was investigated, focusing on its impact on K + absorption, Na + absorption, and the K + /Na + ratio. Similar to findings in tomato [ 17 ], tobacco [ 47 ], maize [ 48 ], and Arabidopsis [ 18 , 19 ], CmoDREB2A was found to enhance salt tolerance by increasing K + absorption and decreasing Na + absorption, resulting in an improved K + /Na + ratio ( Fig. 5a–c ).…”

Section: Discussionsupporting

confidence: 82%

“…Similarly, PvDREB1C induces NtNHX4 expression and elevates the K + /Na + ratio in tobacco [ 47 ]. Overexpression of sorghum DREB2 in maize also enhances K + /Na + ratios [ 48 ]. In A. thaliana , Salix matsudana SmDREBA1–4 binds to the AtSOS1 promoter and enhances K + /Na + ratios [ 19 ], while S. caninervis ScDREB5 promotes Na + exodulation by inducing SOS1 / SOS2 / SOS3 expression [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

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Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H2O2 and ABA signaling and K+/Na+ homeostasis

Peng,

Cui,

Wang

et al. 2024

Horticulture Research

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Pumpkin CmoNAC1 enhances salt tolerance in grafted cucumbers. However, the potential interactions with other proteins that may co-regulate salt tolerance alongside CmoNAC1 have yet to be explored. In this study, we identified pumpkin CmoDREB2A as a pivotal transcription factor that interacts synergistically with CmoNAC1 in the co-regulation of salt tolerance. Both transcription factors were observed to bind to each other's promoters, forming a positive regulatory loop of their transcription. Knockout of CmoDREB2A in the root resulted in reduced salt tolerance in grafted cucumbers, whereas overexpression demonstrated the opposite effect. Multiple assays in our study provided evidence of the protein interaction between CmoDREB2A and CmoNAC1. Exploiting this interaction, CmoDREB2A facilitated the binding of CmoNAC1 to the promoters of CmoRBOHD1, CmoNCED6, CmoAKT1;2, and CmoHKT1;1, inducing H2O2 and ABA synthesis and increasing the K+/Na+ ratio in grafted cucumbers under salt stress. Additionally, CmoNAC1 also promoted the binding of CmoDREB2A to CmoHAK5;1/CmoHAK5;2 promoters, further contributing to the K+/Na+ homeostasis. In summary, these findings reveal a crucial mechanism of CmoNAC1 and CmoDREB2A forming a complex enhancing salt tolerance in grafted cucumbers.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H2O2 and ABA signaling and K+/Na+ homeostasis

Peng,

Cui,

Wang

et al. 2024

Horticulture Research

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“…The transcription factors, as regulatory proteins, exert vital functions in drought tolerance by synchronizing the signaling network and gene expression under stress. Some of the most critical players in the abscisic acid pathway are drought-responsive element binding (DREB) proteins that are a part of AP2/ethylene response factor transcription factors that bind to promoters of some family genes needed to be expressed under abiotic stresses [ 30 ]. The drought tolerance of NAC TFs has been demonstrated in various crops, such as rice [ 31 ] and soybean [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Physiological Characteristic Changes and Transcriptome Analysis of Maize (Zea mays L.) Roots under Drought Stress

Zou,

Tan,

Huang

et al. 2024

International Journal of Genomics

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Water deficit is a key limiting factor for limiting yield in maize (Zea mays L.). It is crucial to elucidate the molecular regulatory networks of stress tolerance for genetic enhancement of drought tolerance. The mechanism of drought tolerance of maize was explored by comparing physiological and transcriptomic data under normal conditions and drought treatment at polyethylene glycol- (PEG-) induced drought stress (5%, 10%, 15%, and 20%) in the root during the seedling stage. The content of saccharide, SOD, CAT, and MDA showed an upward trend, proteins showed a downward trend, and the levels of POD first showed an upward trend and then decreased. Compared with the control group, a total of 597, 2748, 6588, and 5410 differentially expressed genes were found at 5%, 10%, 15%, and 20% PEG, respectively, and 354 common DEGs were identified in these comparisons. Some differentially expressed genes were remarkably enriched in the MAPK signaling pathway and plant hormone signal transduction. The 50 transcription factors (TFs) divided into 15 categories were screened from the 354 common DEGs during drought stress. Auxin response factor 10 (ARF10), auxin-responsive protein IAA9 (IAA9), auxin response factor 14 (ARF14), auxin-responsive protein IAA1 (IAA1), auxin-responsive protein IAA27 (IAA27), and 1 ethylene response sensor 2 (ERS2) were upregulated. The two TFs, including bHLH 35 and bHLH 96, involved in the MAPK signal pathway and plant hormones pathway, are significantly upregulated in 5%, 10%, 15%, and 20% PEG stress groups. The present study provides greater insight into the fundamental transcriptome reprogramming of grain crops under drought.

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“…roots of maize seedlings were immersed in 10% polyethylene glycol (PEG) 6000 (Izadi-Darbandi et al, 2023). For cold-stress treatment, maize seedlings were grown at 4˚C.…”

Section: Core Ideasmentioning

confidence: 99%

Overexpression of the late embryonic genesis abundant protein MGL3 improves the drought tolerance of maize (Zea mays L.)

Liu,

Chen,

Gao

et al. 2024

Crop Science

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With the increasing severity of climate change, drought has become a global issue that seriously restricts the development and production of crops. Maize (Zea mays L.) is one of the major food crops in the world. Therefore, the demand for drought‐tolerant maize varieties has been rapidly increasing in the market. Recent studies have found that late embryogenesis abundant (LEA) proteins are involved in plant responses to various osmotic stresses such as drought and salt stress. Thus, we hypothesized that LEA genes may provide similar stress tolerance abilities in maize. We isolated ZmMGL3 of the LEA gene family and developed transgenic maize plants overexpressing ZmMGL3 using Agrobacterium‐mediated transformation. Then, we conducted physiological and biochemical evaluations of the transgenic maize plants exposed to drought stress. Under drought stress (10% polyethylene glycol 6000), the transgenic maize plants showed improved germination rate, seed vigor, radicle length, root length at the seedling stage, and wilting degree after drought and rewatering compared to the wild‐type plants. The transgenic plants also accumulated more catalase, superoxide dismutase, peroxidase, hydrogen peroxide, and superoxide radicals compared to the wild‐type plants. These results indicate that ZmMGL3 enhances drought resistance in maize plants by reducing the content of reactive oxygen species in the leaves and can be used as a candidate gene for the development of drought‐tolerant maize varieties.

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Introducing sorghum DREB2 gene in maize (Zea mays L.) to improve drought and salinity tolerance

Cited by 7 publications

References 48 publications

Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H2O2 and ABA signaling and K+/Na+ homeostasis

Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H2O2 and ABA signaling and K+/Na+ homeostasis

Physiological Characteristic Changes and Transcriptome Analysis of Maize (Zea mays L.) Roots under Drought Stress

Overexpression of the late embryonic genesis abundant protein MGL3 improves the drought tolerance of maize (Zea mays L.)

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