Introduction of Genetic Material into Plant Cells

Caplan, Allan; Herrera‐Estrella, Luis; Inzé, Dirk; Haute, Eddy Van; Montagu, Marc Van; Schell, Jeff; Zambryski, Patricia

doi:10.4324/9780429050329-38

Cited by 6 publications

(6 citation statements)

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“…This plasmid contained a precise deletion of the complete internal T-DNA region, only conserving the T-DNA borders. By a simple co-integration step, it became possible to integrate any gene of interest (GOI) between both T-DNA borders (Caplan et al, 1983;Zambryski et al, 1983;Zambryski et al, 2013). Around the same time, another strategy to facilitate the cloning of a specific GOI was introduced: the binary vector system.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium strains and strain improvement: Present and outlook

Saeger

Park

Chung

et al. 2021

Biotechnology Advances

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Section: Introductionmentioning

confidence: 99%

Agrobacterium strains and strain improvement: Present and outlook

Saeger

Park

Chung

et al. 2021

Biotechnology Advances

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“…The most prominent ECR-type of Rhodobacterales and rhizobia are RepABC-type plasmids, whose occurrence is restricted to Alphaproteobacteria [54]. Tumour-inducing (Ti) RepABC plasmids of Agrobacterium have been intensively studied since the 1970s, and their derivatives were later used as biotechnological workhorses for plant genetics in the pre-CRISPR-Cas9 era [55,56]. However, the model system is still of scientific interest and mutations within the partitioning protein RepB have recently been correlated with the transition from a single copy to a higher copy number plasmid [57].…”

Section: Novel Insights Into the Biology Of Repabc-type Plasmids From...mentioning

confidence: 99%

Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales

et al. 2022

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The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae . Here, we show that two large natural RepABC-type plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co-cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co-culture.

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“…Para resolver este problema se han desarrollado dos estrategias de clonación que facilitan la manipulación del ADN de grandes plásmidos Ti. La primera utiliza un vector intermediario (Caplan et al, 1983;Horsch et al, 1984). En este caso, se extrae la región ADN-T del plásmido Ti y se clona en un plásmido pequeño de E. coli, pBR322, que es relativamente fácil de manipular in vitro.…”

Section: Transformación De Plantas Mediada Porunclassified

Mejora genética en el cultivo del tabaco. Mecanismos moleculares y regulación

Álvarez

Falconí

Paredes³

2019

RIULCB

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Mejora genética en el cultivo del tabaco. Mecanismos moleculares y regulación Genetic improvement in tobacco crops. Molecular mechanisms and regulation RESUMEN Las enfermedades producidas por hongos fitopatógenos son una de las principales causas de pérdidas de las cosechas desde que el hombre comenzó a cultivar plantas. Hasta el presente, estas enfermedades han sido controladas fundamentalmente mediante: el mejoramiento de la resistencia de las plantas a través de programas de selección y cruzamiento, el empleo de productos agroquímicos y la adecuada rotación de los cultivos. Sin embargo, estas estrategias no son suficientes para el control de las enfermedades causadas por hongos porque los largos períodos de tiempo necesarios para realizar los cruzamientos y retro-cruzamientos así como la selección de variedades comerciales, son suficientes para que aparezcan nuevas razas de hongos con diferentes niveles de virulencia; por otro lado, el uso de productos agroquímicos resulta costoso y eventualmente pierden eficiencia con la evolución del patógeno, además de ser potencialmente peligrosos para el ecosistema. Aplicando las modernas técnicas de la biotecnología vegetal, así como la ingeniería genética, es posible obtener plantas que se autoprotejan contra los hongos o los grupos de hongos en un corto tiempo. Se estudia el caso tabaco, como modelo para entender los mecanismos moleculares de transferencia y regulación que podrán ser aplicados en cultivos de alta importancia económica como la papa.

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Introduction of Genetic Material into Plant Cells

Cited by 6 publications

References 1 publication

Agrobacterium strains and strain improvement: Present and outlook

Agrobacterium strains and strain improvement: Present and outlook

Fatal affairs – conjugational transfer of a dinoflagellate-killing plasmid between marine Rhodobacterales

Mejora genética en el cultivo del tabaco. Mecanismos moleculares y regulación

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