Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

Ulijn, Rein V.; Flitsch, Sabine L.; Margetts, Graham; Brazendale, Ian; Ebert, Cynthia; Martin, Luigi De; Linda, Paolo; Verdelli, Silvia; Gardossi, Lucia

doi:10.1016/j.tet.2003.10.125

Cited by 15 publications

(12 citation statements)

References 17 publications

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“…The accessibility of PEGA polymers to larger enzymes was greatly improved by modifying their formulation. Part of the acrylamide monomers in PEGA 1900 were substituted with monomers containing permanent positively‐charged functions (Scheme ) 29, 30…”

Section: Supports For Solid Phase Biocatalysismentioning

confidence: 99%

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

Rabelo

Rossell

Rocha

et al. 2012

Biotechnology Progress

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Sugarcane bagasse was subjected to steam pretreatment impregnated with hydrogen peroxide. Analyses were performed using 2(3) factorial designs and enzymatic hydrolysis was performed at two different solid concentrations and with washed and unwashed material to evaluate the importance of this step for obtaining high cellulose conversion. Similar cellulose conversion were obtained at different conditions of pretreatment and hydrolysis. When the cellulose was hydrolyzed using the pretreated material in the most severe conditions of the experimental design (210 °C, 15 min and 1.0% hydrogen peroxide), and using 2% (w/w) water-insoluble solids (WIS), and 15 FPU/g WIS, the cellulose conversion was 86.9%. In contrast, at a milder pretreatment condition (190 °C, 15 min and 0.2% hydrogen peroxide) and industrially more realistic conditions of hydrolysis (10% WIS and 10 FPU/g WIS), the cellulose conversion reached 82.2%. The step of washing the pretreated material was very important to obtain high concentrations of fermentable sugars.

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Section: Supports For Solid Phase Biocatalysismentioning

confidence: 99%

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

Rabelo

Rossell

Rocha

et al. 2012

Biotechnology Progress

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“…This is in agreement with the results of Basso et al, who showed that the accessibility of penicillin G acylase (pI ¼ 5.2-5.4) into PEGA (i.e., crosslinked acrylamide and poly(ethylene glycol)) resins could be improved by introducing positive charges into the polymer; this result was caused by the electrostatic interactions between polymer and enzyme. [28,29] S. C. De Smedt et al / Microparticles As Protease Sensors The percentages indicate the relative decrease in red fluorescence upon adding trypsin (compared to the addition of ANHT). To the left of the dashed line are beads having a positively charged inner PE layer.…”

Section: Influence Of the Number Of Pe Layers On The Cleavage Of P1 Bmentioning

confidence: 99%

Evaluation of Encoded Layer‐By‐Layer Coated Microparticles As Protease Sensors

Stubbe

Gevaert

Derveaux

et al. 2008

Adv Funct Materials

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Proteases are important pharmaceutical targets for new drugs because of their involvement in numerous disease processes. This study evaluates whether photophysically encoded microparticles carrying fluorescently labeled protease substrates (peptides) at their surface show potential for detecting proteases in a sample. Layer‐by‐layer (LbL) polyelectrolyte coatings, containing a red‐labeled peptidic trypsin substrate, are carefully designed and applied at the surface of the encoded microparticles. The peptide‐loaded LbL coatings lose their red fluorescence upon incubation in a trypsin solution, indicating that LbL‐coated microparticles show potential to screen for the presence of active proteases in biological samples.

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“…The paper shows that accessibility can be improved for penicillin G amidase by introducing permanent charges into the polymer. 13 …”

Section: Novel Resins and Linkersmentioning

confidence: 99%

Combinatorial Chemistry Online

Terrett

2004

Combinatorial Chemistry - an Online Journal

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Introduction of permanently charged groups into PEGA resins leads to improved biotransformations on solid support

Cited by 15 publications

References 17 publications

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

Enhancement of the enzymatic digestibility of sugarcane bagasse by steam pretreatment impregnated with hydrogen peroxide

Evaluation of Encoded Layer‐By‐Layer Coated Microparticles As Protease Sensors

Combinatorial Chemistry Online

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