Introduction to a Special Issue of the Journal of Immunological Methods: Building Global Resource Programs to Support HIV/AIDS Clinical Trial Studies

Sánchez, Ana M.; Denny, Thomas N.; O’Gorman, Maurice R.G.

doi:10.1016/j.jim.2014.05.016

Cited by 8 publications

(2 citation statements)

References 29 publications

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“…Cryopreservation below −130°C is widely used in clinical trials, 11,12 multicenter studies, 10 and in almost every biological and biomedical research field. It enables long-term storage, 24,31 maintaining functionality and viability.…”

Section: Discussionmentioning

confidence: 99%

Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality

Angel

et al. 2016

Biopreservation and Biobanking

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Cryopreservation of biological materials such as cells, tissues, and organs is a prevailing topic of high importance. It is employed not only in many research fields but also in the clinical area. Cryopreservation is of great importance for reproductive medicine and clinical studies, as well as for the development of vaccines. Peripheral blood mononuclear cells (PBMCs) are commonly used in vaccine research where comparable and reliable results between different research institutions and laboratories are of high importance. Whereas freezing and thawing processes are well studied, controlled, and standardized, storage conditions are often disregarded. To close this gap, we investigated the influence of suboptimal storage conditions during low-temperature storage on PBMC viability, recovery, and T cell functionality. For this purpose, PBMCs were isolated and exposed with help of a robotic system in a low-temperature environment from 0 up to 350 temperature fluctuation cycles in steps of 50 cycles to simulate storage conditions in large biorepositories with sample storage, removal, and sorting functions. After the simulation, the viability, recovery, and T cell functionality were analyzed to determine the number of temperature rises, which ultimately lead to significant cell damage. All studied parameters decreased with increasing number of temperature cycles. Sometimes after as little as only 50 temperature cycles, a significant effect was observed. These results are very important for all fields in which cell cryopreservation is employed, particularly for clinical and multicenter studies wherein the comparability and reproducibility of results play a crucial role. To obtain reliable results and to maintain the quality of the cells, not only the freezing and thawing processes but also the storage conditions should be controlled and standardized, and any deviations should be documented.

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Section: Discussionmentioning

confidence: 99%

Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality

Angel

et al. 2016

Biopreservation and Biobanking

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“…It is expensive, cumbersome and requires a large volume of blood and biosafety level 3 procedures. Cell viability is affected by variable processing and storage conditions, although these have improved with standardized procedures and quality management [ 107 ]. QVOA may underestimate the number of intact proviruses by 25–27-fold.…”

Section: Laboratory Evaluation Of the Reservoirmentioning

confidence: 99%

Barriers to HIV remission research in low‐ and middle‐income countries

Rossouw

Tucker

Zyl

et al. 2017

Journal of the International AIDS Society

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Introduction: HIV eradication and remission research has largely taken place in high-income countries. In low- and middle-income countries (LMIC), there may be factors that have a substantial impact on the size of the latent HIV reservoir and the immunological response to infection. If a curative strategy is to be available to all HIV-infected individuals, these factors must be understood.Methods: We use a scoping review to examine the literature on biological factors that may have an impact on HIV persistence in LMIC. Three databases were searched without date restrictions.Results: Uncontrolled viral replication and higher coinfection prevalence may alter the immunological milieu of individuals in LMIC and increase the size of the HIV reservoir. Differences in HIV subtype could also influence the measurement and size of the HIV reservoir. Immune activation may differ due to late presentation to care, presence of chronic infections, increased gut translocation of bacterial products and poor nutrition.Conclusions: Research on HIV remission is urgently needed in LMIC. Research into chronic immune activation in resource poor environments, the immune response to infection, the mechanisms of HIV persistence and latency in different viral clades and the effect of the microbiological milieu must be performed. Geographic differences, which may be substantial and may delay access to curative strategies, should be identified.

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Quality assessment on the long-term cryopreservation and nucleic acids extraction processes implemented in the andalusian public biobank

et al. 2019

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Introduction to a Special Issue of the Journal of Immunological Methods: Building Global Resource Programs to Support HIV/AIDS Clinical Trial Studies

Cited by 8 publications

References 29 publications

Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality

Toward Optimal Cryopreservation and Storage for Achievement of High Cell Recovery and Maintenance of Cell Viability and T Cell Functionality

Barriers to HIV remission research in low‐ and middle‐income countries

Quality assessment on the long-term cryopreservation and nucleic acids extraction processes implemented in the andalusian public biobank

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