Introduction to biosensors

Iniewski, Krzysztof

doi:10.1017/cbo9781139629539.010

Cited by 1 publication

(1 citation statement)

References 12 publications

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“…Electrical stimulation can be applied via neuromodulation devices [4] such as retinal [5] and cochlear implants or electrodes implanted in the brain such as deep brain stimulation [6]. The use of such devices involves direct contact between electrodes and tissue and often requires additional wires connected to an external power supply [7,8]. Alternative wireless methods to stimulate excitable cells encompass a range of approaches, including optogenetics, photothermal stimulation using materials with strong absorption in the near-infrared spectrum, and photomechanical stimulation, where light induces physical changes in materials to generate mechanical forces [9].…”

Section: Introductionmentioning

confidence: 99%

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca²⁺ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Rienmüller,

Shrestha,

Polz

et al. 2024

IEEE Trans. Biomed. Eng.

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This study aims to explore the potential of organic electrolytic photocapacitors (OEPCs), an innovative photovoltaic device, in mediating the activation of native voltagegated Cav1.2 channels (ICa,L) in guinea pig ventricular cardiomyocytes. Methods: Whole-cell patch-clamp recordings were employed to examine light-triggered OEPC mediated ICa,L activation, integrating the channel's kinetic properties into a multicompartment cell model to take intracellular ion concentrations into account. A multidomain model was additionally incorporated to evaluate effects of OEPC-mediated stimulation. The final model combines external stimulation, multicompartmental cell simulation, and a patch-clamp amplifier equivalent circuit to assess the impact on achievable intracellular voltage changes. Results: Light pulses activated ICa,L, with amplitudes similar to voltage-clamp activation and high sensitivity to the L-type Ca 2+ channel blocker, nifedipine. Light-triggered ICa,L inactivation exhibited kinetic parameters comparable to voltage-induced inactivation. Conclusion: OEPC-mediated activation of ICa,L demonstrates their potential for nongenetic optical modulation of cellular physiology potentially paving the way for the development of innovative therapies in cardiovascular health. The integrated model proves the light-mediated activation of ICa,L and advances the understanding of the interplay between the patch-clamp amplifier and external stimulation devices. Significance: Treating cardiac conduction disorders by minimalinvasive means without genetic modifications could advance therapeutic approaches increasing patients' quality of life compared with conventional methods employing electronic devices.

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Section: Introductionmentioning

confidence: 99%

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca²⁺ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Rienmüller,

Shrestha,

Polz

et al. 2024

IEEE Trans. Biomed. Eng.

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Introduction to biosensors

Cited by 1 publication

References 12 publications

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca²⁺ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca²⁺ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

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Introduction to biosensors

Cited by 1 publication

References 12 publications

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca2+ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

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Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca²⁺ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices

Shedding Light on Cardiac Excitation: In Vitro and In Silico Analysis of Native Ca²⁺ Channel Activation in Guinea Pig Cardiomyocytes Using Organic Photovoltaic Devices