Introduction to Surface Plasmon Resonance

Biosensors for detection of Low-Density Lipoprotein and its modified forms

“…For each measurement, the surface is primed with a suitable buffer solution to create a baseline on the SPR curve (Green et al, 2000). It is of vital relevance to have a reliable baseline before the capturing event starts (Tudos & Schasfoort, 2008). Once the biomolecule comes into contact with the surface, rapid adsorption occurs resulting in an increase in the SPR angle and is followed by a plateau in the adsorption profile due to saturation of the surface (Fig.…”

“…Often SPR measurements are carried out to determine the kinetics of a binding process. For realistic results it is vital to prevent immobilization from changing the ligand in a way that would influence its strength or affinity towards the target component (Tudos & Schasfoort, 2008).…”

“…The difference between the SPR 1 to SPR 2 gives an indication on the extent of adsorption (Fig. 3b), and the positive gradient of the SPR adsorption curve determines the rate of adsorption (Damos et al, 2004;Green et al, 2000;Tudos & Schasfoort, 2008). Often SPR measurements are carried out to determine the kinetics of a binding process.…”

Characterization of interactions formed by the SNARE Syntaxin4 protein and the Sec/Munc protein Munc18c

“…The final binding curve is obtained by subtracting the reference curve from the test curve (Shiau et al, 2008;Tudos & Schasfoort, 2008). Figure 4.2 shows the experimental set up and a typical binding curve (sensorgram) from an SPR experiment.…”

“…(Table 4.1) et al, 2008). This change in refractive index is measured as response units (RU) and is proportional to the amount of mass deposited on the detection layer (Tudos & Schasfoort, 2008;Jason-Moller et al, 2006).…”