Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

Rákosy-Tican, Elena; Thieme, Ramona; König, Janine; Nachtigall, Marion; Hammann, Thilo; Dénes, Tünde-Éva; Kruppa, Klaúdia; Molnár-Láng, Márta

doi:10.3389/fpls.2020.00699

Cited by 40 publications

(44 citation statements)

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“…One of the aims of this study was to compile a set of new candidate R genes (File S2) for further investigation. Association of a candidate R gene with the cultivar specific resistance against some pathogen provides opportunity for further study and inclusion in potato breeding programs or other biotechnological approaches for plant improvement (e.g., [3,[79][80][81][82][83][84][85]). Further investigation of candidate R genes may be conducted with the aid of reverse genetics (suppression or increased expression of a targeted gene under testing combined with phenotype evaluation).…”

Section: Discussionmentioning

confidence: 99%

“…Marker-assisted selection (MAS) is efficiently exploited in selecting desirable genotypes with resistance genes (R genes) and stacking several R genes to facilitate the breeding of new cultivars with durable resistance [1][2][3]. MAS can increase the efficiency of conventional breeding, and it is applicable for traits conferred by both major qualitative genes and quantitative trait loci (QTLs).…”

Section: Introductionmentioning

confidence: 99%

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NLR Genes Related Transcript Sets in Potato Cultivars Bearing Genetic Material of Wild Mexican Solanum Species

et al. 2021

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The long history of potato breeding includes the numerous introgressions of resistance genes from many wild species of South and Central America as well as from cultivated species into the breeding genepool. Most R genes belong to the NLR family with nucleotide-binding site–leucine-rich repeat. The aim of this research concerns an evaluation of NLR genes expression in transcriptomes of three potato cultivars (Evraziya, Siverskij, Sudarynya), which combine genetic material from wild and cultivated potato species, and each bears intragenic markers of RB/Rpi-blb1/Rpi-sto1 genes conferring broad-range resistance to late blight. The transcriptomes of the cultivars were compared before and 24 h after the Phytophthora infestans inoculation. The induction of RB/Rpi-blb1/Rpi-sto1 transcript after 24 h of inoculation was detected in the resistant cultivars Siverskij and Sudarynya but not in susceptible cv. Evraziya. This demonstrates the importance of transcriptomic assay for understanding the results of marker-assisted selection and phenotyping. Interestingly, assembling the transcriptomes de novo and analysis with NLR-parser tool revealed significant fractions of novel NLR genes with no homology to the reference genome from 103 (cv. Siverskij) to 160 (S. stoloniferum, 30514/15). Comparison of novel NLRs demonstrated a relatively small intersection between the genotypes that coincided with their complex pedigrees with several interspecific hybridization events. These novel NLRs may facilitate the discovery of new efficient R genes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

NLR Genes Related Transcript Sets in Potato Cultivars Bearing Genetic Material of Wild Mexican Solanum Species

et al. 2021

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“…In order to protect potato crops for a more prolonged period, stacking of multiple resistance genes within one potato variety is a promising way forward (Tan et al 2010;Vleeshouwers et al 2011;Jo et al 2014;Ghislain et al 2019;Rakosy-Tican et al 2020). Moreover, Rodewald and Trognitz (2013) argued that combining resistance genes with a different mode of action would make it less probable that P. infestans can overcome these new stacks of resistance genes with one single mutation in its virulence spectrum, extending the time period in which these resistance stacks are expected to be effective in commercial (organic) potato production.…”

Section: Breeding For Late Blight Resistancementioning

confidence: 99%

Breeding Late Blight Resistant Potatoes for Organic Farming—a Collaborative Model of Participatory Plant Breeding: the Bioimpuls Project

et al. 2021

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In organic potato production, the need for varieties with durable late blight resistance developed through classical breeding programmes is urgent. Besides late blight resistance, other variety characteristics needed in organic potato production are early canopy closure for weed suppression and good tuber dormancy to eliminate the need for (chemical) sprouting inhibition during storage, amongst others. This paper is a unique example of collaboration between researchers, farmers and professional breeders of both large, medium and small breeding companies. The aim of the resulting breeding project, Bioimpuls, was to provide a substantial impulse to both the organic and conventional potato breeding sector by enlarging the access to various sources of late blight resistance. The Bioimpuls activities include providing true seed populations for variety selection with five available sources of R-genes against Phytophthora infestans, early and advanced introgression breeding with six new R-genes, and education and communication. The results achieved over the 11-year period (2009–2019) are analysed. Many true seed populations containing multiple resistance genes are produced and selected, and a constant flow of breeding clones is entering the evaluation and positioning trials of companies. However, it will still take a considerable amount of time before varieties with stacked resistance genes will replace the new resistant single gene varieties entering the market in the next few years. Five out of six new sources of R-genes need more years of backcrossing before they are ready for commercial use. Bioimpuls successfully introduced a training course for farmer breeders, and published a manual for potato breeding.

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“…While single R gene resistance can be rapidly overcome by pathogen evolution, the presence of several R genes could possibly favor quantitative late blight resistance in potato [27]. The development of molecular markers has recently enabled stacking such resistance genes into elite backgrounds and pre-breeding lines in tetraploid potato [28,29] and accelerated their introgression into breeding lines.…”

Section: Introductionmentioning

confidence: 99%

Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains

et al. 2021

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Potato virus Y (PVY) and Phytophthora infestans (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging. Breeding operations for vegetatively propagated crops can lead to genotype mislabeling that, in turn, reduces genetic gains. Low-density and low-cost molecular marker assessment for phenotype prediction and quality control is a viable option for breeding programs. Here, we report on the development of kompetitive allele specific PCR (KASP) markers for LB and PVY resistance, and for routine quality control assessment of different breeding populations. Two KASP markers for LB resistance and two for PVY Ryadg were validated with an estimated assay power that ranged between 0.65 and 0.88. The developed QC KASP markers demonstrated the capability of discriminating tetraploid calls in breeding materials, including full-sibs and half-sibs. Routine implementation of the developed markers in a breeding program would assist with better allocation of resources and enable precise characterization of breeding material, thereby leading to increased genetic gains.

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Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines

Cited by 40 publications

References 54 publications

NLR Genes Related Transcript Sets in Potato Cultivars Bearing Genetic Material of Wild Mexican Solanum Species

NLR Genes Related Transcript Sets in Potato Cultivars Bearing Genetic Material of Wild Mexican Solanum Species

Breeding Late Blight Resistant Potatoes for Organic Farming—a Collaborative Model of Participatory Plant Breeding: the Bioimpuls Project

Kompetitive Allele Specific PCR (KASP) Markers for Potato: An Effective Tool for Increased Genetic Gains

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