Invasive Methicillin-Resistant Staphylococcus aureus USA500 Strains from the U.S. Emerging Infections Program Constitute Three Geographically Distinct Lineages

Frisch, Michael B.; Castillo-Ramírez, Santiago; Petit, Robert A.; Farley, Monica M.; Ray, Susan M.; Albrecht, Valérie; Limbago, Brandi; Hernandez, Jeranfel; See, Isaac; Satola, Sarah W.; Read, Timothy D.

doi:10.1128/msphere.00571-17

Cited by 28 publications

(26 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This clone showed higher instability in the PVL-and SAK-encoding phages than the other two clones. The variability of these phage genes was also shown in another MRSA linage USA500 (Frisch et al, 2018), unveiling the potential importance of horizontal gene transfer in evolution of pathogenic MRSA.…”

Section: Discussionmentioning

confidence: 81%

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

Pang

Zhang

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Sequence type 59 (ST59) is a predominant clonal lineage of community-acquired, methicillin-resistant Staphylococcus aureus (CA-MRSA) in Asia. Despite its increasing clinical relevance in China, the evolution and geographic expansion of ST59 has been relatively uncared for. Previous study has shown that ST59 was the predominant clone in food-related MRSA in China. This study compared the genomes of 87 clonal complex (CC) 59 S. aureus isolates sourced from food chain and infection cases to reconstruct the molecular evolution and geographical spread of ST59. Accordingly, three major sub-clades of ST59 were identified and these did not correlate with isolation source or location. Phylogenetic analysis estimated that ST59 in mainland China diverged from a most common recent ancestor around 1974, and most of the cases of cross-country transmission occurred between 1987 and 2000. Notably, two recent events of crosscountry transmission through the food chain were observed, the isolates from these events diverged within relatively short time intervals. These isolates also showed high similarity in terms of their core genome, accessory genes, and antibiotic resistance patterns. These findings provide a valuable insight into the potential route of ST59 expansion in China and indicate a need for robust food chain surveillance to prevent the spread of this pathogen.

show abstract

Section: Discussionmentioning

confidence: 81%

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

Pang

Zhang

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Divergent lineages of CA-MRSA dominate different regions of the world, although USA300 is the most successful clone worldwide [1] , [2] , [4] , [5] , [6] . Other examples include those from livestock, food production chains and farm-related humans in Europe (such as spa t127) [30] ; USA500 (CC8) from CA and HA infections in the United States [31] ; and ST8/ spa t008/SCC mec IVe, which often displays levofloxacin resistance and a 1 Mbp genomic inversion in Russia [15] , [16] , [32] . CA-MRSA/J has its own unique features: SCC mec IVl with spj (described above) and the phage-related chromosomal island SaPI (SAPIj50) carrying a tst region, which may have originated from SaPIm1/n1 of the ST5/SCC mec II HA-MRSA New York/Japan clone [10] .…”

Section: Discussionmentioning

confidence: 99%

Fatal case of ST8/SCCmecIVl community-associated methicillin-resistant Staphylococcus aureus infection in Japan

Ishitobi

Wan

Khokhlova

et al. 2018

New Microbes and New Infections

View full text Add to dashboard Cite

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) with ST8/SCCmecIV threatens human health. However, its pathogenesis remains unclear. ST8 CA-MRSA (CA-MRSA/J) with SCCmecIVl, which carries the large LPXTG-motif–containing putative adhesin gene, spj, has emerged in Japan. We present the first reported case of death from CA-MRSA/J. The patient was a 64-year-old woman with iliopsoas abscesses complicated by septic pulmonary embolism and multiorgan abscesses. Vancomycin, arbekacin, daptomycin and rifampicin were ineffective. CA-MRSA/J was resistant to erythromycin, clindamycin and antiseptics and was invasive in a HEp-2 cell assay, in contrast to skin-derived villous-adherent CA-MRSA/J. This suggests the strongly invasive pathotype of CA-MRSA/J.

show abstract

“…However, some of these species have a clear clinical and economic relevance, as they are a frequent cause of infection in humans, livestock, and domestic animals. Although the genus is very well known for the human opportunist pathogen S. aureus, which is famous worldwide as major source of nosocomial infections (Challagundla, Reyes et al 2018, Frisch, Castillo-Ramirez et al 2018, there are some other species such as S.…”

Section: Manuscript To Be Reviewedmentioning

confidence: 99%

Peer Review #1 of "Phylogenomics picks out the par excellence markers for species phylogeny in the genus Staphylococcus (v0.1)"

2018

View full text Add to dashboard Cite

Although genome sequencing has become a very promising approach to conduct microbial taxonomy, few labs have the resources to afford this especially when dealing with data sets of hundreds to thousands of isolates. The goal of this study was to identify the most adequate loci for inferring the phylogeny of the species within the genus Staphylococcus; with the idea that those who cannot afford whole genome sequencing can use these loci to carry out species assignation confidently. We retrieved 177 orthologous groups (OGs) by using a genome-based phylogeny and an average nucleotide identity analysis. The top 26 OGs showed topologies similar to the species tree and the concatenation of them yielded a topology almost identical to that of the species tree. Furthermore, a phylogeny of just the top 7 OGs could be used for species assignment. We sequenced four staphylococcus isolates to test the 26 OGs and found that these OGs were far superior to commonly used markers for this genus. On the whole, our procedure allowed identification of the most adequate markers for inferring the phylogeny within the genus Staphylococcus. We anticipate that this approach will be employed for the identification of the most suitable markers for other bacterial genera and can be very helpful to sort out poorly classified genera.

show abstract

Invasive Methicillin-Resistant Staphylococcus aureus USA500 Strains from the U.S. Emerging Infections Program Constitute Three Geographically Distinct Lineages

Cited by 28 publications

References 69 publications

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

The Genomic Context for the Evolution and Transmission of Community-Associated Staphylococcus aureus ST59 Through the Food Chain

Fatal case of ST8/SCCmecIVl community-associated methicillin-resistant Staphylococcus aureus infection in Japan

Peer Review #1 of "Phylogenomics picks out the par excellence markers for species phylogeny in the genus Staphylococcus (v0.1)"

Contact Info

Product

Resources

About