INVect - a novel polycationic reagent for transient transfection of mammalian cells

Püngel, Sebastian; Veiczi, Miklos; Welsink, Tim; Faust, Daniel; Vater, Vanessa; Levison, Derek; Möller, Uwe; Weglöhner, Wolfgang

doi:10.1186/1753-6561-7-s6-p26

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“…The CAP-T cells were shown to be a production platform for reporter proteins SEAP and GFP [34], but especially the expression of various complex molecules proved the competitiveness of the cell line compared to CHO and HEK293 cells [35]. CAP cells were tested and certified according to Pharmacopoeia guidelines and approved by authorities for production of proteins and vaccines.…”

Section: Capmentioning

confidence: 99%

Human cell lines for the production of recombinant proteins: on the horizon

2015

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Section: Capmentioning

confidence: 99%

Human cell lines for the production of recombinant proteins: on the horizon

2015

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Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

Gutiérrez-Granados

Cervera

Kamen

et al. 2018

Critical Reviews in Biotechnology

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Transient gene expression (TGE) in animal cell cultures has been used for almost 30 years to produce milligrams and grams of recombinant proteins, virus-like particles and viral vectors, mainly for research purposes. The need to increase the amount of product has led to a scale-up of TGE protocols. Moreover, product quality and process reproducibility are also of major importance, especially when TGE is employed for the preparation of clinical lots. This work gives an overview of the different technologies that are available for TGE and how they can be combined, depending on each application. Then, a critical assessment of the challenges of large-scale transient transfection follows, focusing on suspension cell cultures transfected with polyethylenimine (PEI), which is the most widely used methodology for transfection. Finally, emerging opportunities for transient transfection arising from gene therapy, personalized medicine and vaccine development are reviewed.

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INVect - a novel polycationic reagent for transient transfection of mammalian cells

Cited by 2 publications

References 2 publications

Human cell lines for the production of recombinant proteins: on the horizon

Human cell lines for the production of recombinant proteins: on the horizon

Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics

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