Inverse relationship between Sec14l3 mRNA/protein expression and allergic airway inflammation

Shan, Lihua; Kawakami, Takao; Asano, Shigetaka; Noritake, Shinobu; Yoshimoto, Daisuke; Yamashita, Koh; Kikkawa, Hideo; Kinoshita, Mine; Matsubara, Shigeki

doi:10.1016/j.ejphar.2009.06.055

Cited by 16 publications

(15 citation statements)

References 43 publications

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“…Moreover, COS-1 cells transduced with a Sec14l3 expression vector similarly excrete the Sec14l3 protein into the culture medium [26]. Consistent with these data, our previous proteomic experiments using rat bronchoalveolar lavage (BAL) fluids identified the Sec14l3 protein as a component of BAL fluid [16].…”

Section: Discussionsupporting

confidence: 71%

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Sec14l3 Is Specifically Expressed in Mouse Airway Ciliated Cells

et al. 2011

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Airway epithelium is a key component for airway integrity. Previously, we found that expression of the Sec14l3 gene that encodes a 45-kDa secretory protein is inversely associated with the progression of experimentally induced airway inflammation and degeneration/necrosis of alveolar epithelium. In this report, using in situ hybridization we demonstrated that the ciliated cells in mouse lung selectively express Sec14l3 mRNA. In a three-dimensional culture of mouse tracheal epithelial cells, levels of the Sec14l3 mRNA correlated with the differentiation of ciliated cells. Intranasal infection of adult mice with influenza virus resulted in a 20-fold, progressive decrease in Sec14l3 mRNA expression over 10 days post infection. These results enhance the potential value of Sec14l3 as a ciliated epithelial cell-specific biomarker for the progression of airway inflammations such as airway viral infection and asthma.

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Section: Discussionsupporting

confidence: 71%

“…Previously, we demonstrated a clear inverse association of rat Sec14l3 gene expression with the progression of allergic airway inflammation in a Brown Norway allergic rat model [16]. Sec14l3 encodes a water-soluble 45-kDa secreted protein [17,18].…”

Section: Introductionmentioning

confidence: 98%

Sec14l3 Is Specifically Expressed in Mouse Airway Ciliated Cells

et al. 2011

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“…However, this is one of the few studies to examine clinically detectable airway responses and lung cytokine, leukocyte, and mediator responses to a brief exercise or allergic challenge in rats at a time interval (10 min postchallenge) that mimics the phenomenon of EIB in humans (59). The exercise challenge was designed to reflect the 6 -8 min of intense exercise recommended for stimulating EIB in humans (12), and much shorter in duration than is typically used in rodent exercise studies classified as "brief" or "single-bout" (e.g., 22,25).…”

Section: Discussionmentioning

confidence: 98%

A rat model of exercise-induced asthma: a nonspecific response to a specific immunogen

Kodesh¹,

Zaldivar²,

Schwindt³

et al. 2011

American Journal of Physiology-Regulatory, Integrative and Comp

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Exercise-induced bronchoconstriction (EIB) is common; however, key aspects of its pathogenesis are still unclear. We investigated the feasibility of adapting an established animal model of asthma to investigate the earliest stages of EIB. The hypothesis was that a single exposure to a normally innocuous, and brief, exercise challenge could trigger EIB symptoms in rats previously sensitized to ovalbumin (OVA) but otherwise unchallenged. Brown-Norway rats were sensitized by intraperitoneal injection of OVA at 0 and 2 wk. At week 3, animals were exposed to either aerosolized OVA (SS) or exercise (EXS). A trained, blinded, clinical observer graded EIB by respiratory sounds. Plasma and lung cytokine levels were analyzed. No control rats with or without exercise (EX, CON) showed evidence of EIB. Eighty percent of the SS group demonstrated abnormal breath sounds upon exposure to aerosolized OVA. Approximately 30% of EXS rats sensitized to OVA but exposed only to exercise had abnormal breath sounds. Lung tissue levels of TNF-α, IL-1α, growth-related oncogene/keratinocyte/chemoattractant, and IFN-γ were significantly higher (P < 0.001) in the SS group, relative to all other groups. Changes in most of these cytokines were not notable in the EXS rats, suggesting a different mechanism of EIB. Remarkably, IFN-γ, but not the other cytokines measured, was significantly elevated following brief exercise in both sensitized and unsensitized rats. Exercise led to detectable breathing sound abnormalities in sensitized rats, but less severe than those observed following classical OVA challenge. Precisely how this immune crossover occurs is not known, but this model may be useful in elucidating essential mechanisms of EIB.

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“…Indeed, all four of these genes have been associated with cancer lethality, progression or invasiveness(Shipitsin et al 2014;So et al 2014;Su et al 2013;Xu et al 2014), and this finding is interesting in the light of CFTR's own role in suppressing tumour progression(Xie et al 2013). Of the six genes associated exclusively with "Control-like" gene lists (i.e., down-regulated in disease), SEC14L3 (SEC14-like 3 (S. cerevisiae)) stands out for playing an important role in lung development(Hishikawa et al 2013), and is known to have an inverse relationship with allergic airway inflammation(Shan et al 2009), whereas DNALI1 (dynein, axonemal, light intermediate chain 1) is an essential component of motile respiratory cilia(Zariwala et al 2013). The functional significance of the other genes exclusively down-regulated in our clustergram (PTGFR: prostaglandin F receptor, PROS1: protein S (alpha), MAOB: monoamine oxidase B, FABP6:…”

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confidence: 97%

Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators

et al. 2015

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A meta-analysis of 13 independent microarray data sets was performed and gene expression profiles from cystic fibrosis (CF), similar disorders (COPD: chronic obstructive pulmonary disease, IPF: idiopathic pulmonary fibrosis, asthma), environmental conditions (smoking, epithelial injury), related cellular processes (epithelial differentiation/regeneration), and non-respiratory "control" conditions (schizophrenia, dieting), were compared. Similarity among differentially expressed (DE) gene lists was assessed using a permutation test, and a clustergram was constructed, identifying common gene markers. Global gene expression values were standardized using a novel approach, revealing that similarities between independent data sets run deeper than shared DE genes. Correlation of gene expression values identified putative gene regulators of the CF transmembrane conductance regulator (CFTR) gene, of potential therapeutic significance. Our study provides a novel perspective on CF epithelial gene expression in the context of other lung disorders and conditions, and highlights the contribution of differentiation/EMT and injury to gene signatures of respiratory disease.

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Inverse relationship between Sec14l3 mRNA/protein expression and allergic airway inflammation

Cited by 16 publications

References 43 publications

Sec14l3 Is Specifically Expressed in Mouse Airway Ciliated Cells

Sec14l3 Is Specifically Expressed in Mouse Airway Ciliated Cells

A rat model of exercise-induced asthma: a nonspecific response to a specific immunogen

Transcriptome meta-analysis reveals common differential and global gene expression profiles in cystic fibrosis and other respiratory disorders and identifies CFTR regulators

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