Inversion polymorphism in malarial mosquito Anopheles messeae natural populations from the north-eastern region of the range

Rusakova, A. M.; Artemov, Gleb N.; Potapova, N. K.; Stegniy, V. N.

doi:10.1134/s1022795410100042

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“…beklemishevi remains understudied in comparison with An. messeae [ 12 – 19 ]. Moreover, because of the absence of a standard photomap for ovarian nurse cell chromosomes, the adult stage was excluded from the inversion polymorphism analysis.…”

Section: Introductionmentioning

confidence: 99%

A standard photomap of ovarian nurse cell chromosomes and inversion polymorphism in Anopheles beklemishevi

et al. 2018

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BackgroundAnopheles beklemishevi is a member of the Maculipennis group of malaria mosquitoes that has the most northern distribution among other members of the group. Although a cytogenetic map for the larval salivary gland chromosomes of this species has been developed, a high-quality standard cytogenetic photomap that enables genomics and population genetics studies of this mosquito at the adult stage is still lacking.MethodsIn this study, a cytogenetic map for the polytene chromosomes of An. beklemishevi from ovarian nurse cells was developed using high-resolution digital imaging from field collected mosquitoes. PCR-amplified DNA probes for fluorescence in situ hybridization (FISH) were designed based on the genome of An. atroparvus. The DNA probe obtained by microdissection procedures from the breakpoint region was labelled in a DOP-PCR reaction. Population analysis was performed on 371 specimens collected in 18 locations.ResultsWe report the development of a high-quality standard photomap for the polytene chromosomes from ovarian nurse cells of An. beklemishevi. To confirm the suitability of the map for physical mapping, several PCR-amplified probes were mapped to the chromosomes of An. beklemishevi using FISH. In addition, we identified and mapped DNA probes to flanking regions of the breakpoints of two inversions on chromosome X of this species. Inversion polymorphism was determined in 13 geographically distant populations of An. beklemishevi. Four polymorphic inversions were detected. The positions of common chromosomal inversions were indicated on the map.ConclusionsThe study constructed a standard photomap for ovarian nurse cell chromosomes of An. beklemishevi and tested its suitability for physical genome mapping and population studies. Cytogenetic analysis determined inversion polymorphism in natural populations of An. beklemishevi related to this species’ adaptation.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-2657-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%