Invertebrate Tissue Culture Methods

Mitsuhashi, Jun

doi:10.1007/978-4-431-67875-5

Cited by 45 publications

(19 citation statements)

References 88 publications

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“…Cells inoculated with IAPV tested positive over several subcultures. In bee cell culture medium, WH2 cells from larvae and pupae attached to the plate substrate and cell migration, and multiplication was observed in as early as 24 h. The monolayer cells were composed of two types of epithelial cells similar to descriptions from leafhopper Kimura (1984), Mitsuhashi (1976Mitsuhashi ( , 2002, Wayadande and Fletcher (1998). Cell cultures were labeled as "AmWH".…”

Section: Cell Line Characterizationmentioning

confidence: 99%

Medium for development of bee cell cultures (Apis mellifera: Hymenoptera: Apidae)

Hunter

2009

In Vitro Cell.Dev.Biol.-Animal

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A media for the production of cell cultures from hymenopteran species such as honey bee, Apis mellifera L. (Hymenoptera: Apidae) was developed. Multiple bee cell cultures were produced when using bee larvae and pupae as starting material and modified Hert-Hunter 70 media. Cell culture systems for bees solves an impasse that has hindered efforts to isolate and screen pathogens which may be influencing or causing colony collapse disorder of bees. Multiple life stages of maturing larvae to early pupae were used to successfully establish cell cultures from the tissues of the head, thorax, and abdomen. Multiple cell types were observed which included free-floating suspensions, fibroblast-like, and epithelia-like monolayers. The final culture medium, WH2, was originally developed for hemipterans, Asian citrus psyllid, Diaphorina citri, and leafhopper, Homalodisca vitripennis cell cultures but has been shown to work for a diverse range of insect species such as bees. Bee cell cultures had various doubling times at 21-23 degrees C ranging from 9-15 d. Deformed wing virus was detected in the primary explanted tissues, which tested negative by rt-PCR for Israeli acute paralysis virus (IAPV), Kashmir bee virus, acute bee paralysis virus, and black queen cell virus. Culture inoculation with IAPV from an isolate from Florida field samples, was detectable in cell cultures after two subcultures. Cell culture from hymenoptera species, such as bees, greatly advances the approaches available to the field of study on colony collapse disorders.

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Section: Cell Line Characterizationmentioning

confidence: 99%

Medium for development of bee cell cultures (Apis mellifera: Hymenoptera: Apidae)

Hunter

2009

In Vitro Cell.Dev.Biol.-Animal

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“…Considering the inadequacy of the available growth media several attempts have been made to improvise the composition by adding supplements in isolation as well as in [24] and as the growthpromoting substances [59]. Considering the importance of inorganic salts for the maintenance of ionic balance and osmotic pressure [61], researchers have used KCl, MgSO 4 , MgCl 2 , and CaCl 2 at concentrations ranging from 0.9 to 3 g/l to supplement the required quantity in the growth medium [39,48,49].…”

Section: Organic and Inorganic Supplements To Improve Growth Of Shrimmentioning

confidence: 99%

Establishment of Shrimp Cell Lines: Perception and Orientation

2012

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Development of continuous shrimp cell lines for effective investigation on shrimp viruses remains elusive with an arduous history of over 25 years. Despite presenting challenges to researchers in developing a cell line, the billion dollar aquaculture industry is under viral threat. Advances in molecular biology and various gene transfer technologies for immortalization of cells have resulted in the development of hundreds of cell lines from insects and mammals, but yet not a single cell line has been developed from shrimp and other marine invertebrates. Though improved growth and longevity of shrimp cells in vitro could be achieved by using modified growth media this did not make any leap to spontaneous transformation; probably due to the fact that shrimp cells inhibited neoplastic transformations. Oncogenic induction and immortalization are considered as the possible ways, and an exclusive medium for shrimp cell culture and an appropriate mode of transformation are crucial. In this review status of shrimp cell line development and its future orientation are discussed.

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“…The tissue culture cells at passage five were exposed to colcemid, trypsinised, and processed according to standard methods (Mitsuhashi, 2002). Briefly, the cells were incubated until 70-80% confluency, and 0.1 µg/ ml of colchine was added for approximately 4-6 h. Then, the cells were harvested for hypotonic treatment, which was applied for less than 15 min.…”

Section: Chromosome Specimen Preparationmentioning

confidence: 99%

Karyotype analysis of the acute fibrosarcoma from chickens infected with subgroup J avian leukosis virus associated with v-srconcogene

Xuan

Chen

et al. 2016

Avian Pathology

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To understand the cytogenetic characteristics of acute fibrosarcoma in chickens infected with the subgroup J avian leukosis virus associated with the v-src oncogene, we performed a karyotype analysis of fibrosarcoma cell cultures. Twenty-nine of 50 qualified cell culture spreads demonstrated polyploidy of some macrochromosomes, 21 of which were trisomic for chromosome 7, and others were trisomic for chromosomes 3, 4, 5 (sex chromosome w), and 10. In addition, one of them was trisomic for both chromosome 7 and the sex chromosome 5 (w). In contrast, no aneuploidy was found for 10 macrochromosomes of 12 spreads of normal chicken embryo fibroblast cells, although aneuploidy for some microchromosomes was demonstrated in five of the 12 spreads. The cytogenetic mosaicism or polymorphism of the aneuploidy in the acute fibrosarcoma described in this study suggests that the analysed cells are polyclonal. ARTICLE HISTORY

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Invertebrate Tissue Culture Methods

Cited by 45 publications

References 88 publications

Medium for development of bee cell cultures (Apis mellifera: Hymenoptera: Apidae)

Medium for development of bee cell cultures (Apis mellifera: Hymenoptera: Apidae)

Establishment of Shrimp Cell Lines: Perception and Orientation

Karyotype analysis of the acute fibrosarcoma from chickens infected with subgroup J avian leukosis virus associated with v-srconcogene

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