Investigating diverse methods for inducing sporulation in endophytic fungi

Mattoo, Aroosa Jan; Nonzom, Skarma

doi:10.48130/sif-2022-0016

Cited by 2 publications

(1 citation statement)

References 61 publications

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“…The lowest proportion of sterile mycelia was 11-16% from Trachycarpus fortunei [94], with the highest proportion of 54% from Quercus ilex [95]. Gnavi et al [96] isolated 88 endophytes from Posidonia oceanica, of which 21 (23%) were sterile mycelia, while Mattoo and Nonzom [97] isolated 681 endophytes from Ephedra gerardiana, but most (499 isolates, 73.2%) were sterile mycelia.…”

Section: Identificationmentioning

confidence: 99%

Why Are There So Few Basidiomycota and Basal Fungi as Endophytes? A Review

Rungjindamai,

Jones

2024

JoF

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A review of selected studies on fungal endophytes confirms the paucity of Basidiomycota and basal fungi, with almost 90% attributed to Ascomycota. Reasons for the low number of Basidiomycota and basal fungi, including the Chytridiomycota, Mucoromycota, and Mortierellomycota, are advanced, including isolation procedure and media, incubation period and the slow growth of basidiomycetes, the identification of non-sporulating isolates, endophyte competition, and fungus–host interactions. We compare the detection of endophytes through culture-dependent methods and culture-independent methods, the role of fungi on senescence of the host plant, and next-generation studies.

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Section: Identificationmentioning

confidence: 99%

Why Are There So Few Basidiomycota and Basal Fungi as Endophytes? A Review

Rungjindamai,

Jones

2024

JoF

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Characterization of a Diaporthe toxica Strain: Growth, Spore Formation, Phomopsin-A, and Alkaloids Production on Lupins

Buccioni,

Rossi,

Serio

et al. 2024

Toxins

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The growing interest in vegetable proteins, namely those derived from lupins, has raised concerns over potential safety risks associated with these food products. Lupin serves as the main host for the mycotoxin-producing fungus called Diaporthe toxica. This species, which is associated with animal diseases, has been scarcely characterized. Recently, phomopsin-A (PHO-A), the main mycotoxin produced by D. toxica, was found to be harmful to humans. Therefore, this study aimed at characterizing D. toxica growth and spore formation both in vitro and on lupin samples. In addition, the production of PHO-A and alkaloids was investigated on lupin beans by using three different inoculation methods. Particularly, growth and spore production were evaluated on different media, while PHO-A and alkaloid production were determined by means of µSPE extraction followed by UHPLC-MS/MS and HPLC-MS/MS, respectively. The results have demonstrated differences in growth on different media, with potato and oat-flakes-based media being the best options. Conversely, D. toxica was not able to produce spores on agar media, but only on lupin beans. Moreover, a thorough analysis of PHO-A production revealed an increase over time, reaching values up to 1082.17 ppm after 21 days on artificially rehydrated samples. On the other side, the analysis of alkaloids revealed impressive results, as this species produced great quantities of the quinolizidine alkaloids (QA) that are normally present in lupin seeds such as lupanine, sparteine, multiflorine, and hydroxylupanine. On balance, considering these results, different metabolic pathways were demonstrated in D. toxica, which are not adequately described in the existing literature. These data are of paramount importance to deepen the knowledge about a fungal species that is important to ensure the safety of lupin and lupin-based products.

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Investigating diverse methods for inducing sporulation in endophytic fungi

Cited by 2 publications

References 61 publications

Why Are There So Few Basidiomycota and Basal Fungi as Endophytes? A Review

Why Are There So Few Basidiomycota and Basal Fungi as Endophytes? A Review

Characterization of a Diaporthe toxica Strain: Growth, Spore Formation, Phomopsin-A, and Alkaloids Production on Lupins

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