Investigating In vitro micro propagation of (Saponaria officiale L) by nodal culture with sucrose supplement

Abstract

Medicinal soap, scientifically known as (saponaria officinalis L), is a perennial plant belonging to the family of carnation and a valuable medicinal and industrial plant. The origin of this plant is the Mediterranean region. The medicinal properties of plant soap include blood filtering, elimination of rheumatism, gout, urinary tract and skin diseases, cure cancer, eczema and tuberculosis. The purpose of this study was to investigate medicinal soap plant micro-propagation using explantinternodes in a medium containing 50 grams of sucrosein the presence of hormone levels of BA (0 -0.05 -0.2-0.5) and naphthalene acetic acid (0 -0.05 -0.2-0.5). Different factors appraisedincluded fresh weight, root and stem length, leaf area, length and width of callus, number of stems and roots, embryogenetic plants, aerial root, root hair, green leaves, red leaves, lost leaves, number of nodesand stem produced from each node and color of callus. The mean comparison showed that the freehormone medium conducive to the increment of root length, the medium containing 0.5 mg/l BA and 0.5 mg/lnaphthalene acetic acid (NAA) increased the number of red leaves, length of plant, as well as the number of stems growing from each node,the medium without BA, containing 0.5 mg/l NAA increased the number of green leaves, the number of nodes, length and widthof leaves, medium containing 0.5 mg/l BA and NAA 0.05 mg per liter gave rise to dead leaves, medium containing 0.5 milligrams per liter BA and without naphthalene acetic acid affected the number of roots, plant fresh weight and length of callus, the medium0.05mg/l BA and 0.05mg/l naphthalene acetic acid increased the length of leaves and the number of embryo genetic plants. The medium containing Mg / liter BA with 0.2 mg/l naphthalene acetic acid had an increasing effect on hair roots and the medium containing 0.05 mg/l BA and 0.5 mg per liter NAA were effective in increasing the width of the callus.