Investigating Macromolecular Complexes in Solution by Small Angle X-Ray Scattering

Calculation of Small-Angle Scattering Patterns

“…In this chapter, the scattering of an incident beam of radiation by a scattering potential will be assumed [7,[11][12][13][14]. A schematic view of the scattering process is shown in Figure 1.…”

“…, which is directly related to the particle shape. This information is contained in the scattering amplitude A k , as shown below [7,[12][13][14]:…”

“…In this description a plane monochromatic wave (far-field approximation) is scattered by a finite potential field Vr ! , and the wave function that expresses this phenomenon is a superposition of the transmitted plane wave e ikz and scattered wave [7,[12][13][14].…”

“…Therefore, the obtained results can be affected by the experimental technique itself [7]. Scattering/diffraction methods, on the other hand, can be used for systems directly in solution or in the amorphous matrix, with minimum interaction of the radiation with the matter [7,8]. These methods, namely, small-angle scattering (SAS) either with neutrons (SANS) or X-rays (SAXS), static light scattering (SAS), etc., can provide useful information about the structure of the investigated system.…”

“…However, these methods demand the use of special experimental conditions like measurements in vacuum and the use of coating agents. Therefore, the obtained results can be affected by the experimental technique itself [7]. Scattering/diffraction methods, on the other hand, can be used for systems directly in solution or in the amorphous matrix, with minimum interaction of the radiation with the matter [7,8].…”