Investigating neuronal activity with planar microelectrode arrays: achievements and new perspectives

Morin, Fabrice O.; Takamura, Yuzuru; Tamiya, Eiichi

doi:10.1263/jbb.100.131

Cited by 134 publications

(116 citation statements)

References 110 publications

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“…Mapping LTD in a cortical circuit MEA technology represents a valuable tool to record responses in numerous sites simultaneously for a long time (Oka et al, 1999;Morin et al, 2005;Hofmann and Bading, 2006). We have used a 64-channel MEA system, MED64, in mouse brain slices to study the spatial distribution of synaptic transmission and LTD in the ACC.…”

Section: Discussionmentioning

confidence: 99%

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

et al. 2012

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Long-term depression (LTD) is a key form of synaptic plasticity important in learning and information storage in the brain. It has been studied in various cortical regions, including the anterior cingulate cortex (ACC). ACC is a crucial cortical region involved in such emotion-related physiological and pathological conditions as fear memory and chronic pain. In the present study, we used a multielectrode array system to map cingulate LTD in a spatiotemporal manner within the ACC. We found that low-frequency stimulation (1 Hz, 15 min) applied onto deep layer V induced LTD in layers II/III and layers V/VI. Cingulate LTD requires activation of metabotropic glutamate receptors (mGluRs), while L-type voltage-gated calcium channels and NMDA receptors also contribute to its induction. Peripheral amputation of the distal tail impaired ACC LTD, an effect that persisted for at least 2 weeks. The loss of LTD was rescued by priming ACC slices with activation of mGluR1 receptors by coapplying (RS)-3,5-dihydroxyphenylglycine and MPEP, a form of metaplasticity that involved the activation of protein kinase C. Our results provide in vitro evidence of the spatiotemporal properties of ACC LTD in adult mice. We demonstrate that tail amputation causes LTD impairment within the ACC circuit and that this can be rescued by activation of mGluR1.

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Section: Discussionmentioning

confidence: 99%

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

et al. 2012

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“…These two commerciallyavailable MEA systems make possible the wide use of the technique on various biological preparations, including acutely-dissociated brain slices (e.g., hippocampus, cortex, hypothalamus, substantia nigra, amygdala, superior colliculus, and brain stem), cultured slices (e.g., hippocampus, spinal cord, suprachiasmatic nucleus, cerebellum, and septohippocampal co-culture), retina, myocardial (ventricular and atrial slices) and smooth muscle from mammals [2,[17][18][19][20][21][22] .…”

Section: Stable Field Excitatory Postsynaptic Potentials (Fepsps)mentioning

confidence: 99%

“…testing and behavioral studies [2,20] . Compared to traditional electrophysiology, the MEA technique is superior in that it enables: (1) gathering large amounts of spatial information on the internal dynamics of networks with multisite recordings [42,43] ; (2) long-term analysis of the spatiotemporal distribution of network-level electrical activity [20,44] ; (3) multisite stimulation and recording within one slice [20,42] ;…”

Section: Examples Of Ltd Recording Using Meas Besidesmentioning

confidence: 99%

Use of multi-electrode array recordings in studies of network synaptic plasticity in both time and space

Liu

Chen

et al. 2012

Neurosci. Bull.

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Simultaneous multisite recording using multi-electrode arrays (MEAs) in cultured and acutely-dissociated brain slices and other tissues is an emerging technique in the field of network electrophysiology. Over the past 40 years, great efforts have been made by both scientists and commercial concerns, to advance this technique. The MEA technique has been widely applied to many regions of the brain, retina, heart and smooth muscle in various studies at the network level.The present review starts from the development of MEA techniques and their uses in brain preparations, and then specifically concentrates on the use of MEA recordings in studies of synaptic plasticity at the network level in both the temporal and spatial domains. Because the MEA technique helps bridge the gap between single-cell recordings and behavioral assays, its wide application will undoubtedly shed light on the mechanisms underlying brain functions and dysfunctions at the network level that remained largely unknown due to the technical difficulties before it matured.

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“…It was shown that microfluidic-based systems have a great potential in application for single-cell analysis [1,2] and instant analyses or so called point-of-care (POC) diagnostics [3][4][5]. In this respect, microfluidics systems provide conditions that are comparable to in vivo conditions [1].…”

Section: Introductionmentioning

confidence: 99%

“…Fast evaporation of fluid (e.g., biological media, chemical reagents) in the microchannels may cause premature cell death especially in long-term culture assays [5]. To reduce the risk of culture medium evaporation, encapsulation of microchannels is considered an essential requirement.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of three-dimensional SU-8 microchannels by proton beam writing for microfluidics applications: Fluid flow characterisation

Alshehri¹,

Palitsin²,

Webb³

et al. 2015

Nuclear Instruments and Methods in Physics Research Section B:

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Investigating neuronal activity with planar microelectrode arrays: achievements and new perspectives

Cited by 134 publications

References 110 publications

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

Plasticity of Metabotropic Glutamate Receptor-Dependent Long-Term Depression in the Anterior Cingulate Cortex after Amputation

Use of multi-electrode array recordings in studies of network synaptic plasticity in both time and space

Fabrication of three-dimensional SU-8 microchannels by proton beam writing for microfluidics applications: Fluid flow characterisation

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