Investigating supramolecular systems using Förster resonance energy transfer

Teunissen, Abraham J. P.; Pérez-Medina, Carlos; Meijerink, Andries; Mulder, Willem J. M.

doi:10.1039/c8cs00278a

Cited by 136 publications

(119 citation statements)

References 168 publications

(185 reference statements)

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“…Their FRET efficiencies (E) were calculated based on equation 1. [8] The efficiencies reached the maximum value and remained almost unchanged as the concentrations increased to 10 mm. As a result, the maximum efficiencies (95.8 % for DBT, 96.7 % for NR, 98.3 % for DCM, 87.4 % for DCM2, and 85.4 % for DCJTB) were used for the FRET distance calculations [Equation (1)]:…”

Section: Angewandte Chemiementioning

confidence: 92%

“…The overlap integrals of the absorption spectra of DBT, NR, DCM, DCM2, and DCJTB with the emission spectrum of TPE-C 10 TEAB micelles were firstly obtained and shown in Figure 5 a. [8] The emission spectra of TPE-C 10 TEAB micelles in the absence and presence of the five acceptors with different concentrations were measured and plotted in Figure 5 b. The emission intensities of TPE donors dramatically decreased with increasing the concentrations of DBT, NR, DCM, DCM2, and DCJTB.…”

Section: Angewandte Chemiementioning

confidence: 99%

“…Fçrster resonance energy transfer (FRET) is widely used as a spectroscopic ruler because the FRET distance between donor and acceptor can be calculated accurately. [8] Currently, the FRET rulers have been applied to measure the micellar size and aggregation number. [9] Furthermore, FRET can establish the relationship between the distance and the distribution of donor-acceptor pairs in the micelles.…”

mentioning

confidence: 99%

“…In principle, if the fluorescent donors can be anchored in the center of the micellar interior, the FRET distance could be used as a unique spectrometer to detect solubilization locations of solubilized acceptors. [8] Unfortunately, the aggregationcaused quenching (ACQ) effect usually happens when the fluorescent donors are aggregated in the center of the micellar interior. [10] Assuming that the aggregation-induced emission (AIE)active fluorophores [11] are anchored in the center of the micellar interior and serve as FRET donors, the AIE-active fluorophores could emit intensely in the aggregated state, instead of the ACQ effect.…”

mentioning

confidence: 99%

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Measurement of Solubilization Location in Micelles Using Anchored Aggregation‐Induced Emission Donors

Guan

Yang

2020

Angewandte Chemie

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Solubilization locations play a critical role in developing advanced surfactants and improving solubilization power in micelle‐based applications. However, the current polarity‐based techniques for measuring solubilization locations could come to conflicting conclusions. The key challenge is the unpredictable polarities in the micellar microenvironment. Now, an approach that is independent of micellar polarities is used to measure solubilization locations by covalently linking tetraphenylethylene (TPE) to the alkyl chain end of cationic surfactants. The solubilization locations of solubilized acceptors in the TPE‐cored spherical micelles were accurately measured by calculating the Förster resonance energy transfer distance between anchored TPE donors and solubilized acceptors. Solubilization locations of solubilized substances in the micellar interior and at the micellar surface depend on their size and hydrophobicity, respectively.

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Section: Angewandte Chemiementioning

confidence: 92%

Section: Angewandte Chemiementioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Measurement of Solubilization Location in Micelles Using Anchored Aggregation‐Induced Emission Donors

Guan

Yang

2020

Angewandte Chemie

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“…Because of its strict distance dependency, FRET usually occurs only between molecules separated by less than 10 nm ( Figure 1B). This endows FRET with a unique nanometer sensitivity, which is very advantageous for its application to study proximities and interactions of various biomacromolecules, either in vivo or in vitro [4][5][6]. Especially in living cells, several FRET microscopy techniques were implemented to study (supra)molecular structures, events, and reactions with unprecedented spatial resolution, and with only minimal interference with the studied system [7,8].…”

Section: Introductionmentioning

confidence: 99%

FRET Microscopy in Yeast

2019

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Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells. Here, we summarize existing FRET experiments and biosensors applied in yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, two important models of fundamental biomedical research and efficient platforms for analyses of bioactive molecules. We aim to provide a practical guide on suitable FRET techniques, fluorescent proteins, and experimental setups available for successful FRET experiments in yeasts.

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Future‐Oriented Advanced Diarylethene Photoswitches: From Molecular Design to Spontaneous Assembly Systems

et al. 2022

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cycloreversion of DAE under light irradiation produces many exciting functional effects, such as changes in photophysical and photochemical properties, [12] changes in the shape or mechanical properties of the solid, [13] electrical conductivity, [7b,14] and the generation of reactive oxygen species (ROS). [15] This type of photochromic compound can be considered a stilbene derivative. At this time, the most widely known and systematically studied DAE derivative structure contains two aryl groups and a benzene ring (usually two identical or different thiophene units with low aromatic stabilization energy [16] ) and a five-membered ring of perfluorocyclopentene and cyclopentene units [17] where the ethene bridge is located, which significantly improve photochromism. [18] In essence, the DAE photoisomerization process is a reversible electrocyclic reaction of the central 6π-electron systems. [2] Under light irradiation, DAE can rapidly and reversibly transform between its open and closed forms, and that transformation is accompanied by a color change caused by a change in the π-conjugation distribution. [2] The important thing is that the colorless opened-and colored closed-ring isomers are both thermally stable; that is, cyclization and cycloreversion do not proceed spontaneously in the dark; they need to be driven by external light irradiation at specific wavelengths. [2,19] Compared with classic lightresponsive molecules such as azobenzene, [20] the light intensity required to drive the isomerization of DAE is relatively low. Moreover, even after multiple cycles, the photochromic performance does not show significant fatigue in either the solution or solid state. [2,18b,21] DAE research to this point can be roughly divided into three stages in chronological order: early exploration of DAE's molecular structure and theory, nearly two decades of purpose-oriented molecular structure modification research, and about ten years of research into practical applications of DAE in assembly systems. Research before about 2000 focused mainly on developing more abundant DAE-derivative structures and advancing the systematic understanding of DAE's physical and chemical properties and switching performance. To regulate its switching performance and give it new functionality, many strategies for modifying the ethene bridge and aryl groups of DAE have been proposed. [22] In the first two research stages, the mechanisms and structural modification of DAE isomerization were thoroughly and systematically explored, and countless important Diarylethene (DAE) photoswitch is a new and promising family of photochromic molecules and has shown superior performance as a smart trigger in stimulus-responsive materials. During the past few decades, the DAE family has achieved a leap from simple molecules to functional molecules and developed toward validity as a universal switching building block. In recent years, the introduction of DAE into an assembly system has been an attractive strategy that enables the photochromic behavior of the b...

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Investigating supramolecular systems using Förster resonance energy transfer

Cited by 136 publications

References 168 publications

Measurement of Solubilization Location in Micelles Using Anchored Aggregation‐Induced Emission Donors

Measurement of Solubilization Location in Micelles Using Anchored Aggregation‐Induced Emission Donors

FRET Microscopy in Yeast

Future‐Oriented Advanced Diarylethene Photoswitches: From Molecular Design to Spontaneous Assembly Systems

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