Investigating the biocompatibility of two new heavy intraocular dyes for vitreoretinal surgery with an isolated perfused vertebrate retina organ culture model and a retinal ganglion cell line

Januschowski, Kai; Mueller, Sebastian; Spitzer, Martin S.; Hoffmann, J.; Schramm, Christian; Schultheiß, Maximilian; Bartz-Schmidt, Karl-Ulrich; Szurman, Peter

doi:10.1007/s00417-011-1895-2

Cited by 25 publications

(29 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2), in agreement with the well-documented neuroprotective effects of PEG [64]. Apart from the study on ARPE cells, electrophysiological evaluation did not reveal any detrimental effects of MembraneBlue-Dual on retinal ganglion cells in vitro, with application times up to after 5 min [65]. Combined, these results indicate MembraneBlue-Dual to be biocompatible and safe for clinical use.…”

Section: Figsupporting

confidence: 79%

Dyes for Eyes™: Hydrodynamics, Biocompatibility and Efficacy of ‘Heavy' (Dual) Dyes for Chromovitrectomy

Mohr

Bruinsma²,

Oellerich³

et al. 2013

Ophthalmologica

View full text Add to dashboard Cite

As epiretinal membranes (ERMs), the internal limiting membrane (ILM) and the vitreous cortex are essentially transparent tissues, or translucent structures, nontraumatic removal may be challenging in various types of macular surgery. Vital dyes stain these thin tissues, thus allowing for better visualization of these structures during vitrectomy and selective ‘membrane peeling' from the underlying retina. To avoid swirling of the dye within the fluid-filled vitreous cavity, and to better target the dye onto the macula, a fluid-air exchange is commonly performed. However, this may jeopardize visualization of the macula during peeling due to clouding of the posterior lens capsule, and may lead to postoperative visual field defects. Recently, a new dye solution for staining the ERM and ILM simultaneously has been developed that circumvents the need for fluid-air exchange, i.e. MembraneBlue-Dual™. This paper will focus on the hydrodynamics and biocompatibility of this ‘heavy' dual dye and its efficacy for staining of the ILM and/or ERMs during posterior segment surgery in a multicenter clinical setting.

show abstract

Section: Figsupporting

confidence: 79%

Dyes for Eyes™: Hydrodynamics, Biocompatibility and Efficacy of ‘Heavy' (Dual) Dyes for Chromovitrectomy

Mohr

Bruinsma²,

Oellerich³

et al. 2013

Ophthalmologica

View full text Add to dashboard Cite

show abstract

“…MBB Dual showed also in this study significant effects [45]. Therefore, MBB Dual should also be considered with the background of our results of TB alone [19].…”

mentioning

confidence: 59%

“…Adding heavy water to increase the density of the BBG solution facilitates sedimentation on the retina. The alternatives that exist are adding glucose or PEG 3350 to create a heavier dye [44,45]. The data from our model indicate that epiretinally applied BBG 0.4 ml (0.25 mg/ml) combined with 4.4% glucose has no toxic effects on the retinal function, and seems to be safe for clinical use.…”

mentioning

confidence: 86%

The Retinal Biocompatibility of Dyes in the ex vivo Model of the Isolated Superfused Vertebrate Retina

Lüke

Grisanti²,

Lüke³

2013

Ophthalmologica

View full text Add to dashboard Cite

Background: Peeling of the internal limiting membrane or epiretinal membranes is a successful principle in macular surgery to achieve a functional benefit. Different dyes are used to facilitate the identification of intraocular tissues. The aim of our work was to investigate the retinal tolerance to the different dyes and their solvent carriers to provide valuable data for surgeons in handling for an optimal intraoperative use. Methods: Using the ex vivo model of the isolated superfused vertebrate retina technique, the effects of the dyes were tested on human and bovine retinal function. The retinas were perfused with an oxygen preequilibrated standard solution. The electroretinogram (ERG) was recorded using Ag/AgCl electrodes. After recording stable ERG amplitudes, the dyes brilliant blue G, indocyanine green, trypan blue, patent blue, triamcinolone and their solvent carriers were investigated. Results: Reductions of the ERG amplitudes were found for each tested dye. The effects after application of the dyes were dependent on time and concentration of the applied dyes, which were different for each dye. Conclusion: In part, the ERG has shown strong effects already after a short period of dye application. Surgeons who rely on the intraocular use of the dyes should keep in mind our findings, and the use of some dyes should be limited to selected cases. The well-considered use of the dyes by the surgeons could lead to a better functional outcome and avoid a possible harmful outcome of the surgery after mishandling.

show abstract

“…Several studies showed that ILM peeling was associated with a lower rate of recurrence, and ILM peeling was considered essential to remove the scaffold for further cell proliferation [5,6,13]. The intraoperative use of vital dyes for visualization of the ERM and ILM has been introduced to allow a complete and safe removal of the membranes during the surgery procedure [15][16][17][18][19][20][21][22]32]. The purpose of the present study was to report on the combined use of TB and BBG for staining ERM and ILM during vitrectomy and to describe the histopathological findings.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the 0.15% TB, 0.025% BBG, and 4.00% polyethylene glycol (PEG) combination (MembraneBlue-Dual TM , DORC International, Zuidland, the Netherlands) has been introduced for staining both the ILM and ERM [31,32]. This heavy vital dye is commercially available in Europe.…”

Section: Introductionmentioning

confidence: 99%

The Combination of Trypan Blue and Brilliant Blue G-Assisted Vitrectomy for Macular Pucker: Histopathological Findings

et al. 2018

View full text Add to dashboard Cite

Purpose: To report on the combined use of trypan blue (TB) and brilliant blue G (BBG) for staining the epiretinal membrane (ERM) and internal limiting membrane (ILM) during vitrectomy and to describe the histopathological findings. Methods: 10 surgical specimens were removed from 10 eyes with macular pucker during vitrectomy using a commercially available combination of TB and BBG for ERM and ILM staining and peeling. Specimens were evaluated using light and transmission electron microscopy. Results: In all cases the combination of TB and BBG was useful for identifying and delineating ERM and ILM. No complications related to the use of the dye were observed during or after surgery. Glial cells were present in all specimens. Hyalocytes were observed in 6 cases and myofibroblasts in 3 of them. In 7 cases native vitreous collagen fibrils were found on the ILM, while in 5 specimens newly formed collagen was present. No clinical evidence of toxicity was observed during the 3-month follow-up. Conclusion: The combined use of TB and BBG appeared to be very useful intraoperatively to improve the visualization of ERM and ILM, thus facilitating their complete removal. Anatomical and histopathological findings demonstrated the safety and the efficacy of this vital dye.

show abstract

Investigating the biocompatibility of two new heavy intraocular dyes for vitreoretinal surgery with an isolated perfused vertebrate retina organ culture model and a retinal ganglion cell line

Abstract: ILM Blue and MBB Dual seem to be safe for clinical use for a staining period of up to 120 seconds, probably even up to 320 seconds.

Cited by 25 publications

References 25 publications

Dyes for Eyes™: Hydrodynamics, Biocompatibility and Efficacy of ‘Heavy' (Dual) Dyes for Chromovitrectomy

Dyes for Eyes™: Hydrodynamics, Biocompatibility and Efficacy of ‘Heavy' (Dual) Dyes for Chromovitrectomy

The Retinal Biocompatibility of Dyes in the ex vivo Model of the Isolated Superfused Vertebrate Retina

The Combination of Trypan Blue and Brilliant Blue G-Assisted Vitrectomy for Macular Pucker: Histopathological Findings

Contact Info

Product

Resources

About