Investigating the Quorum Sensing System in Halophilic Bacteria

Tommonaro, Giuseppina; Roberto, Abbamondi Gennaro; Ebru, Toksoy Oner; Nicolaus, Barbara

doi:10.1007/978-3-319-14595-2_7

Cited by 3 publications

(3 citation statements)

References 66 publications

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“…As shown in Figure 5, the absence of luxS could induce an apparent reduction of biofilm formation under hyperosmotic. Notwithstanding the role of LuxS on biofilm formation has been extensively studied in other bacteria (Sewald et al 2007;Giuseppina et al 2015;Jahid et al 2015;Gu et al 2018;Liu et al 2018;Peng et al 2018), the specific function of LuxS in regulating forming biofilm under hyperosmotic stress has not yet been fully understood. Our findings suggested that LuxS had a detectable effect on the phenotypes and biofilm formation for adaption to high osmotic stress.…”

Section: Evaluation Of Luxs In Biofilm Suppressionmentioning

confidence: 99%

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Ling

Tong

et al. 2023

Int J of Dairy Tech

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Cronobacter malonaticus is a highly hyperosmotic‐resistant pathogen classified as Class A pathogen by the Food and Agriculture Organization of the United Nations (FAO). This study explored the roles of LuxS as a quorum‐sensing transcription factor in C. malonaticus to tolerate hyperosmotic stress, illustrating that LuxS contributed to survival, biofilm formation and reduced cellular injury when grown with 5.5% NaCl stress. Comparative proteomics between C. malonaticus WT and ΔluxS demonstrated that differentially expressed proteins (DEPs) were abundant in Fe‐S cluster proteins and oxidoreductases, suggesting a potential cross‐tolerance mediated by LuxS could contribute to resisting hyperosmotic stress. Herein, these findings will provide valuable information on LuxS‐mediated osmotic tolerance to controlling C. malonaticus contamination.

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Section: Evaluation Of Luxs In Biofilm Suppressionmentioning

confidence: 99%

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Ling

Tong

et al. 2023

Int J of Dairy Tech

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“…However, the role of QS in these bacteria has not yet been elucidated, although recently, it has been suggested that it could be related to exopolysaccharide production in the species Halomonas smyrnensis [257]. Regarding the other types of QS signal molecules, AI-2 production has been described in the halophilic bacteria Halobacillus halophilus [258], and production of DKP-type molecules has been characterized in the extremely halophilic archaeon Haloterrigena hispanica [259,260,261].…”

Section: Quorum Sensing Interference In Saline and Hypersaline Envmentioning

confidence: 99%

Saline Environments as a Source of Potential Quorum Sensing Disruptors to Control Bacterial Infections: A Review

2019

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Saline environments, such as marine and hypersaline habitats, are widely distributed around the world. They include sea waters, saline lakes, solar salterns, or hypersaline soils. The bacteria that live in these habitats produce and develop unique bioactive molecules and physiological pathways to cope with the stress conditions generated by these environments. They have been described to produce compounds with properties that differ from those found in non-saline habitats. In the last decades, the ability to disrupt quorum-sensing (QS) intercellular communication systems has been identified in many marine organisms, including bacteria. The two main mechanisms of QS interference, i.e., quorum sensing inhibition (QSI) and quorum quenching (QQ), appear to be a more frequent phenomenon in marine aquatic environments than in soils. However, data concerning bacteria from hypersaline habitats is scarce. Salt-tolerant QSI compounds and QQ enzymes may be of interest to interfere with QS-regulated bacterial functions, including virulence, in sectors such as aquaculture or agriculture where salinity is a serious environmental issue. This review provides a global overview of the main works related to QS interruption in saline environments as well as the derived biotechnological applications.

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“…The researchers reported that cyclo-(L-Pro-L-Val) induced QS system in NTL4 (AHL bioreporter strain) and they suggested that this compound may be involved in QS system of Archaea to survive in such harsh conditions (Tommonaro et al, 2012). Also, autoinducer-2 (AI-2) which provides bacterial communication for interspecies, was detected in Halobacillus halophilus (Tommonaro et al, 2012;Tommonaro et al, 2015). Several studies focused on such compounds examining their potential to be stabilizers of biomolecules, stress protective agents, and halophilic enzymes for biocatalysts (Finore et al, 2011;Poli et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Extremophilic Natrinema versiforme Against Pseudomonas aeruginosa Quorum Sensing and Biofilm

et al. 2020

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Pseudomonas aeruginosa is an opportunistic pathogen that causes high morbidity and mortality rates due to its biofilm form. Biofilm formation is regulated via quorum sensing (QS) mechanism and provides up to 1000 times more resistance against conventional antibiotics. QS related genes are expressed according to bacterial population density via signal molecules. QS inhibitors (QSIs) from natural sources are widely studied evaluating various extracts from extreme environments. It is suggested that extremely halophilic Archaea may also produce QSI compounds. For this purpose, we tested QS inhibitory potentials of ethyl acetate extracts from cell free supernatants and cells of Natrinema versiforme against QS and biofilm formation of P. aeruginosa. To observe QS inhibition, all extracts were tested on P. aeruginosa lasB-gfp, rhlA-gfp, and pqsA-gfp biosensor strains and biofilm inhibition was studied using P. aeruginosa PAO1. According to our results, QS inhibition ratios of cell free supernatant extract (CFSE) were higher than cell extract (CE) on las system, whereas CE was more effective on rhl system. In addition, anti-biofilm effect of CFSE was higher than CE. Structural analysis revealed that the most abundant compound in the extracts was trans 4-(2-carboxy-vinyl) benzoic acid.

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Investigating the Quorum Sensing System in Halophilic Bacteria

Cited by 3 publications

References 66 publications

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Saline Environments as a Source of Potential Quorum Sensing Disruptors to Control Bacterial Infections: A Review

Extremophilic Natrinema versiforme Against Pseudomonas aeruginosa Quorum Sensing and Biofilm

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