Investigating yeast cell responses to oestrogen by electrochemical detection

Chelikani, Venkata; Downard, Alison J.; Kunze, Gotthard; Gooneratne, Ravi; Pasco, Neil; Baronian, Keith

doi:10.1016/j.electacta.2011.11.078

Cited by 7 publications

(4 citation statements)

References 22 publications

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“… 38 The potassium ferricyanide–menadione double-mediator system is widely applied to analyze the redox activity of S. cerevisiae yeast cells and mammalian cells. Moreover, this double-mediator system has been used to investigate a specific enzyme activity or biochemical process in yeast cells, 39,40 single-cell imaging using scanning electrochemical microscopy (SECM), 41 and the application in MFCs, 42 etc. Even though combining mediators can dramatically increase the current magnitude and thus boost the MFC efficiency, there are significant disadvantages.…”

Section: Microbe-electrode Synergymentioning

confidence: 99%

Bio-electrochemical frameworks governing microbial fuel cell performance: technical bottlenecks and proposed solutions

2022

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Section: Microbe-electrode Synergymentioning

confidence: 99%

Bio-electrochemical frameworks governing microbial fuel cell performance: technical bottlenecks and proposed solutions

2022

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“…Chelikani et al . () showed that A. adeninivorans has no detectable intrinsic response to oestrogen compounds, and Vijayan et al . () presented the first transgenic A. adeninivorans strain to express the EBP1 gene from C. albicans .…”

Section: Arxula Adeninivorans As a Biocomponent For Biosensorsmentioning

confidence: 99%

“…Unfortunately they are pathogenic yeasts, which is a potential risk for human health. Chelikani et al (2012) showed that A. adeninivorans has no detectable intrinsic response to oestrogen compounds, and Vijayan et al (2015) presented the first transgenic A. adeninivorans strain to express the EBP1 gene from C. albicans. A single-use electrode, with recombinant oestrogen binding protein for use with a portable potentiostat, can determine oestrogen concentrations in the range of observed environmental concentrations within 2 min.…”

Section: Arxula Adeninivorans As a Biocomponent For Biosensorsmentioning

confidence: 99%

Blastobotrys (Arxula) adeninivorans: a promising alternative yeast for biotechnology and basic research

Malak

Baronian

Kunze

2016

Yeast

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Blastobotrys adeninivorans (syn. Arxula adeninivorans) is a non-conventional, non-pathogenic, imperfect, haploid yeast, belonging to the subphylum Saccharomycotina, which has to date received comparatively little attention from researchers. It possesses unusual properties such as thermo- and osmotolerance, and a broad substrate spectrum. Depending on the cultivation temperature B. (A.) adeninivorans exhibits different morphological forms and various post-translational modifications and protein expression properties that are strongly correlated with the morphology. The genome has been completely sequenced and, in addition, there is a well-developed transformation/expression platform, which makes rapid, simple gene manipulations possible. This yeast species is a very good host for homologous and heterologous gene expression and is also a useful gene donor. Blastobotrys (A.) adeninivorans is able to use a very wide range of substrates as carbon and/or nitrogen sources and is an interesting organism owing to the presence of many metabolic pathways, for example degradation of n-butanol, purines and tannin. In addition, its unusual properties and robustness make it a useful bio-component for whole cell biosensors. There are currently a number of products on the market produced by B. (A.) adeninivorans and further investigation may contribute further innovative solutions for current challenges that exist in the biotechnology industry. Additionally it may become a useful alternative to existing commercial yeast strains and as a model organism in research. In this review we present information relevant to the exploitation of B. (A.) adeninivorans in research and industrial settings. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Electrochemical methods provide the ability to monitor redox processes inside the intact cells, thus reflecting the intracellular state 20,21 . Such experiments are often conducted by transferring whole cells 22,23 , or their parts 24 onto surface of an electrode. To achieve the electron transfer between the electrode and redox centers of the enzymes in the cell, an electroactive mediator is used.…”

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confidence: 99%

Mediated amperometry as a prospective method for the investigation of electroporation

Šimonis

Garjonytė

Stirkė

2020

Sci Rep

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Pulsed electric field effects induced in a membrane, as well as intracellular structures, depend on cell type, field and media parameters. To achieve desired outcomes, membranes should be permeabilized in a controlled manner, and thus efficiency of electroporation should be investigated in advance. Here, we present a framework for using mediated amperometry as a prospective method for the investigation of electroporation and its effects on cellular machinery. Whole-cell sensors with single mediator systems comprised of hydrophilic or lipophilic mediators were successfully employed to investigate membrane permeability as well as cellular responses. Exposure of yeast cells to single electric field pulse (τ = 300 µs, E = 16 kV/cm) resulted in up to tenfold increase of current strength mediated with hydrophilic mediators. Exposure to PEF resulted in decrease of menadione mediated current strength (from 138 ± 15 to 32 ± 15 nA), which could be completely compensated by supplementing electrolyte with NADH.

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Investigating yeast cell responses to oestrogen by electrochemical detection

Cited by 7 publications

References 22 publications

Bio-electrochemical frameworks governing microbial fuel cell performance: technical bottlenecks and proposed solutions

Bio-electrochemical frameworks governing microbial fuel cell performance: technical bottlenecks and proposed solutions

Blastobotrys (Arxula) adeninivorans: a promising alternative yeast for biotechnology and basic research

Mediated amperometry as a prospective method for the investigation of electroporation

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