Investigation of bacterial and fungal agents from cutaneous lesions in canine Leishmaniasis

Abstract

The objective of the present study was to determine the bacterial and fungal agents accompanying with cutaneous lesions of dogs suffering from Canine Visceral Leishmaniasis (CVL). Sterile swap samples were taken from cutaneous lesions inspected of ears, dorsal, collar, pectoral, inguinal and interdigital spaces of 20 dogs, in which diagnosis were based on a combination of clinical symptoms, anti-leishmania antibody titers by use of Immunofluorescence antibody test (IFAT), and dogs were further classified according to the Leishvet Guidelines involving serological, hematological, serum biochemical, and urinalysis. Swap samples were inoculated to culture media for isolation and identification of bacterial and mycotic agents. 48 Staphylococcus aureus, 4 Bacillus cereus, 8 Staphylococcus epidermidis, 24 Candida albicans, 24 Microsporium canis and 4 Aspergillus niger were isolated and identified in both group of dogs. Regarding antibiotic susceptibility test, the isolates were found 100% susceptible to cefoperazone and amoxicillin-clavulanic acid, 53% to danofloxacin, 46% of the isolates were susceptible to enrofloxacin, and all of the isolates were resistant (100%) to penicillin G and gentamicin.In conclusion, the antibacterial and antifungal therapy should be provided in accordance with antibiotic susceptibility tests in skin lesions of dogs suffering with CVL.

The Assessment of antimicrobial activity of cerumen (earwax) and antibiotics against pathogenic bacteria isolated from ear pus samples

“…Different researchers also conducted similar studies and identified different pathogenic bacteria from the ear pus using conventional culturing technique and concluded that P. aeruginosa was the most widely recognized bacteria. 11,20 The antibiotics susceptibility profile for isolated bacterial species were drawn by using agar disc diffusion method while percent value was calculated using CLSI standard values. 19 In the present study, 5 different commercially available antibiotics were evaluated for antimicrobial activity against the prevailing bacterial isolates with the aim to be used for the prevention of different clinical complications.…”

Investigation of Bactericidal effect of Earwax onEscherichia coliandStaphylococcus aureusisolated from skin and stool samples of undergraduate students, Federal University of Agriculture Makurdi, Benue State, Nigeria

“…Earwax (cerumen) is a grey brown or yellowish waxy substance secreted in the outer one third of ear canal and, is a protective barrier between the external environment and deep external auditory canal (Wayne et al, 2020). Chemical composition of earwax includes 60% desquamated, 12-20% saturated and unsaturated fatty acid and 6-10% cholesterol (Yang et al, 2019).…”