Investigation of Deep Eutectic Solvent-Based Microwave-Assisted Extraction and Efficient Recovery of Natural Products

Hong

et al. 2022

Sci Rep

Recently, plant essential oils (EOs) have attracted special attention in plant disease control and food preservation. Since ancient times, essential oils extracted from plants have exhibited many biological characteristics, especially antimicrobial properties. Recent studies have described the potentials of EOs and derivatives to inhibit the growth and reproduction of microorganisms, mainly in response of overwhelming concerns of consumers about food safety. In the context of returning to nature, with the advancement of science and technology and improved living standards, people have begun to seek solutions for food hygiene without chemical additives. Therefore, biological pesticides and plant-oriented chemicals have received special attention from scientists because they are environmentally friendly and nonhazardous, sustainable, and effective alternatives against many noxious phytopathogens. Present study is intended to appraise the fungicidal properties of ginger EOs to combat leaf blight disease of taro, which threatens global taro production. Farmers often hinge on extremely toxic synthetic fungicides to manage diseases, but the residual effects and resistance of chemicals are unavoidable. The microwave-assisted hydrodistillation method was used for ginger EOs extraction and an FTIR (ATR) spectrometer was used to evaluate their chemical composition and citral was identified as most abundant compound (89.05%) in oil. The pathogen isolated from lesions of diseased taro plants was identified as Phytophthora colocasiae and used as test fungus in the present study. Ginger EO was evaluated in-vitro for antifungal properties against mycelium growth, sporangium production, zoospore germination, leaf, and corm necrosis inhibition. Repeated experiments have shown that the concentration of ginger essential oil (1250 ppm) proved to be the lowest dose to obtain 100% inhibition of fungal growth and spore germination, sporangia formation and leaf necrosis assessment. These results are derived from this fungal species and a hypothesis that involves further research on other plant pathogens to demonstrate the overall potency of essential oils. This study references the easy, economic, and environmental management and control of plant diseases using essential oils and byproducts.

Section: Introductionmentioning

confidence: 99%

Fungicidal properties of ginger (Zingiber officinale) essential oils against Phytophthora colocasiae

Hong

et al. 2022

Sci Rep

J of Separation Science

“…On the other hand, the increase of hydroxyl groups makes it easier for alcohols to form intramolecular hydrogen bonds rather than intermolecular hydrogen bonds with choline chloride. The viscosity of the liquid was the measurement of its internal flow resistance, which reflects the intensity of the interaction between molecules in the liquid, and the tiny viscosity make NADES better penetrate the voids in the matrix [29]. The high viscosity of NADESs was mainly due to the formation of hydrogen bond network between HBAs and HBDs.…”

Section: F I G U R E 2 Extraction Yields Of Different Solvents For Or...mentioning

confidence: 99%

Optimization of the extraction process of flavonoids from Trollius ledebouri with natural deep eutectic solvents

Zuo

Geng

et al. 2021

In recent years, natural deep eutectic solvents have been favored greatly due to their environment friendly, mild biological toxicity and simple biodegradability. Natural deep eutectic solvents gradually applied for the extracting bioactive compounds from natural products efficiently. In this study, 20 natural deep eutectic solvents were prepared and their physical and chemical properties were tested. The ultrasonic-assisted extraction method was used to extract flavonoids from Trollius ledebouri and high-performance liquid chromatography-ultraviolet was applied to examine two main bioactive flavonoids (orientin and vitexin). Compared with traditional solvents (water and 60% ethanol solution), natural deep eutectic solvents composed of L(-)-proline and levulinic acid (molar ratio 1:2) show a super extraction efficiency. On this basis, the response surface method was used to optimize the extraction temperature, extraction time, water contents, and solid-liquid ratio. As a consequence, the extraction temperature 60°C, extraction time 18 min, water content 14% (v/v), and the solid-liquid ratio 48 mL⋅g −1 were chosen as the best extraction process. This study shows that natural deep eutectic solvents can effectively extract flavonoids from T. ledebouri, laying a foundation for the further application of natural deep eutectic solvents to extract bioactive compounds from natural products.

Separation Science Plus

“…In comparison to ionic liquids, DESs are cheaper and easier to produce. Due to those and a variety of other favorable properties such as nontoxicity, biocompatibility, high thermal stability, low vapor pressure, they are getting increased attention in many areas of science and technology including various extraction techniques [9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

“…DESs meet the requirements for both MAE and HS-GC -they have a negligible vapor pressure desirable for HS-GC [15] and high polarity [9,16]; thus are able to absorb microwave energy with great efficiency. Considering this, DESs seem to be very promising for MAE as for HS-GC.…”

Section: Introductionmentioning

confidence: 99%

Combined microwave‐assisted extraction and headspace gas chromatography for hexanal determination in fat‐rich food

Vičkačkaitė

Jurkute

Poškus

et al. 2022

The feasibility of a new approach for the determination of volatiles in solid matrices by coupling microwave‐assisted extraction with static headspace gas chromatography has been demonstrated using hexanal as an analyte present in fat‐rich food. Eight polar and low volatility deep eutectic solvents were prepared using microwave heating and examined for hexanal extraction. Choline chloride‐glucose‐water showed the best transition efficiency of hexanal to the headspace and was applied to extract hexanal using microwave‐assisted extraction. Hexanal released from the fat‐rich food is poorly soluble in the hydrophilic deep eutectic solvent choline chloride‐glucose‐water so enters the gas phase and subsequently dissolves in coconut oil fixed above the extraction mixture in a polypropylene capillary. After cooling the capillary was transferred to a headspace vial and headspace gas chromatography was carried out. Under optimized conditions, the limit of detection was 3.1 μg g–1. The developed approach was successfully applied to analyze hexanal in fat‐rich food.