Investigation of Livistona decipiens Leaf Methanol Extract and Evaluation of Antioxidant, Antimicrobial and Cytotoxic Activities

Abstract

Objectives: This study aimed to isolate the polyphenolic constituents from the methanol extract of Livistona decipiens Becc leaves and evaluate the antioxidant, cytotoxic and antimicrobial activities of the total methanol extract and ethyl acetate fraction. Methods: The ethyl acetate and n-butanol fractions of Livistona decipiens leaf methanol extract were subjected separately to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1 H / 13 C NMR). Antioxidant activity was evaluated by DPPH assay, while evaluation of cytotoxicity was done according to MTT cell viability assay. Antimicrobial activity was done by agar diffusion method. Results: seven compounds were isolated from the ethyl acetate and n-butanol fractions, five compounds were identified for the first time from this plant as apigenin-8-C-β-D-glucopyranoside (Vitexin) (1), Quercetin-6-C-β-D-glucopyranoside (2),apigenin-6,8-di-C-β-D-glucopyranoside (Vicenin II) (3), 6-O-methyl Kaempferol 3-O-glucopyranoside (4), luteolin-3-C-gentiobiosyl (5), while two compounds vis; luteolin-6-C-β-Dglucopyranoside (Isoorientin) (6) and luteolin-8-C-β-D-glucopyranoside (Orientin) (7), were isolated for the second time from this species. The ethyl acetate fraction has shown moderate activity against Gram positive and Gram negatvie bacteria, also it showed moderate antioxidant activity with IC50 = 23 ± 0.74 µg/ml when compared to ascorbic acid IC50 = 14.2 ± 0.35 µg/ml. Also ethyl acetate extract has shown cytotoxic activity on MCF-7 cells (human breast cancer cell line), HepG-2 (human hepatocellular carcinoma) and HeLa cells (human cervical cancer cell line), whereas, the methanol extract has shown lower activity. Conclusion: Livistona decipiens have potential medicinal value being rich in polyphenolics and being antioxidant, cytotoxic and antimicrobial drug.

Synthesis, In vitro, and In silico Studies of Methyl Eugenol Derivatives for Plasmodium falciparum Inhibitor

“…The least total flavonoid contentwas detected with petroleum ether extract (6PL) (18.27 ± 0.0.21 mg QE/g). These results are in agreement with that previously reported, 29 where it was found that the polarity of the extraction solvent increases the quantities of the flavonoids extracted. © 2021 the authors.…”

“…The least antioxidant activity is represented in the petroleum ether extract (6PL). These results are in agreement with that reported for P. daemia, 29 as it proved that, the ethanol extract followed by ethyl acetate shows a very close antioxidant activity when compared with the standard, and the least antioxidant activity was exhibited in the n-hexane, and humans can eliminate the free radicals and get relief from oxidative stress effectively by consuming P. daemia even at low concentration. The redox properties of the phenolic compounds play a key role as antioxidants or free radical scavengers which can absorb and neutralize free radicals, triplet oxygen or decomposing peroxides.…”

“…All the NMR data ( 1 H and 13 C) were in accordance with that reported for apigenin 8-C-glucoside (vitexin). 29 Both compounds; P4 and P5 were found to resist the acid hydrolysis and responded to the enzymatic hydrolysis with β-glucosidase affording glucose and apigenin as an aglycone in compound P4, glucose as a sugar, and luteolin as an aglycone in compound P5. P5 (luteolin 6,8-di-C glucoside)Lucenin-2), was isolated as a yellowish powder, appeared as a purple spot which changed to greenish yellow by ammonia vapour and became yellow with Naturstoff reagent (NA).…”

Structural Characterization of Polyphenolics in Livistona chinensis Using HPLC-PDA-MS

“…Traditionally they have been utilized as analgesics and for leukemia, esophageal cancer and nasopharyngeal carcinoma 4 . Reviewing the current literature, it was found that plants belonging to genus Livistona are rich in flavonoids, triterpenes, alkaloids, phenolic acids, amino acids and fatty acids [5][6][7][8][9][10] . In this study, the aqueous ethanolic extract of L. chinensis leaves were qualitatively and quantitatively characterized for the first time by HPLC-PDA-MS/MS, and the total extract was assessed for its anticancer activity.…”

Phenolics Isolation and Chemical Profiling of Livistona australis (R.Br.) Mart. Fruit Extracts; Potent Inhibitors of ?-Amylase and Pancreatic Lipase

“…The phenolic acids derived from benzoic acid; 2-hydroxy benzoic acid (salicylic acid) (8) and 3,4-dihydroxy benzoic acid (protocatechuic acid) (10) presented pseudomolecular ions at m/z 137 and 153, respectively. Their retention times and mass fragmentations proved their identification, based on reference samples.…”