Investigation of platinum nanoparticle properties
against U87 glioblastoma multiforme

Kutwin, Marta; Sawosz, Ewa; Jaworski, Sławomir; Hinzmann, Mateusz; Wierzbicki, Mateusz; Hotowy, Anna; Grodzik, Marta; Winnicka, A.; Chwalibog, A.

doi:10.5114/aoms.2016.58925

Cited by 44 publications

(42 citation statements)

References 34 publications

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“…AZ31 alloy extract (40%) showed significantly increased expression of NF-kB at a regular time interval. A similar result was observed in the TNF-α concentration; AZ31 alloy extract (40%) demonstrated increased expression [52][53][54]58]. Another apoptosis parameter, caspase-3, is considered as a significant marker of inflammation and plays an important role in the apoptosis pathway [57].…”

Section: Discussionsupporting

confidence: 73%

“…Several apoptosis mediators such as caspase-3, TNF-α and NF-kB play an important role in the apoptosis mechanism [49,50]. The apoptosis mediators are activated via transactivating gene expression of anti-apoptosis [51][52][53][54]. Several researchers suggest that the NF-kB pathway is a crucial factor in the cell reaction to apoptotic stimuli [55][56][57].…”

Section: Discussionmentioning

confidence: 99%

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In vitro and in vivo assessment of biocompatibility of AZ31 alloy as biliary stents: a preclinical approach

Song

Qin

et al. 2020

Arch Med Sci

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Introduction: Biomaterial technology due to its lack of or minimal side effects in tissues has great potential. Traditionally biomaterials used were cobalt-chromium, stainless steel and nitinol alloys. Biomaterials such as magnesium (Mg) and zinc (Zn) have good biocompatibility and consequently can be a potential material for medical implants. To date, the effects of AZ31 alloy stent on cell apoptosis are still unclear. The current investigation was designed to determine the effect of AZ31 alloy stent on necrosis and apoptosis of common bile duct (CBD) epithelial cells. Material and methods: We experimented with application of different concentrations of AZ31 alloy stent to primary mouse extrahepatic bile epithelial cells (MEBECs) and estimated the effect on apoptosis and necrotic cells. Apoptosis and pro-apoptosis expression were estimated through real-time PCR. For in vivo protocol, we used rabbits, implanted the AZ31 bile stent, and estimated its effect on the CBD. AZ31 (40%) concentration showed an effect on the apoptotic and necrotic cells. Results: Real-time PCR revealed that AZ31 (40%) concentration increased the apoptotic genes such as NF-κB, caspase-3, Bax and Bax/Bcl-2 ratio as compared to the control group. In the in vivo experiment, AZ31 alloy stents were implanted into the CBD and showed an effect on the alteration the hema tological, hepatic and non-hepatic parameters. Conclusions: To conclude, it can be stated that AZ31 induces apoptosis via alteration in genes including nuclear factor kappa-B (NF-κB), caspase-3, Bax and Bax/Bcl-2 ratio and improved the hematological, hepatic and non-hepatic parameters.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo assessment of biocompatibility of AZ31 alloy as biliary stents: a preclinical approach

Song

Qin

et al. 2020

Arch Med Sci

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“…For instance, PtNPs penetrate cells via diffusion or endocytosis and then form intracellular aggregates [5,[41][42][43]. Platinum NPs inhibit HeLa cell viability and proliferation by activating p53 [41] and inhibiting U87, U118, and U251 glioblastoma tumor cell proliferation [41,44,45]. Cytotoxicity is dependent on PtNP size in Raw 264.7 cells incubated with various concentrations of PtNPs [46].…”

Section: Effects Of Ptnps On Cell Viability and Proliferation Of Thp-mentioning

confidence: 99%

Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis, Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute Monocytic Leukemia Cells

Gurunathan

Jeyaraj

et al. 2020

IJMS

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The thermoplasmonic properties of platinum nanoparticles (PtNPs) render them desirable for use in diagnosis, detection, therapy, and surgery. However, their toxicological effects and impact at the molecular level remain obscure. Nanotoxicology is mainly focused on the interactions of nanostructures with biological systems, particularly with an emphasis on elucidating the relationship between the physical and chemical properties such as size and shape. Therefore, we hypothesized whether these unique anisotropic nanoparticles could induce cytotoxicity similar to that of spherical nanoparticles and the mechanism involved. Thus, we synthesized unique and distinct anisotropic PtNPs using lycopene as a biological template and investigated their biological activities in model human acute monocytic leukemia (THP-1) macrophages. Exposure to PtNPs for 24 h dose-dependently decreased cell viability and proliferation. Levels of the cytotoxic markers lactate dehydrogenase and intracellular protease significantly and dose-dependently increased with PtNP concentration. Furthermore, cells incubated with PtNPs dose-dependently produced oxidative stress markers including reactive oxygen species (ROS), malondialdehyde, nitric oxide, and carbonylated protein. An imbalance in pro-oxidants and antioxidants was confirmed by significant decreases in reduced glutathione, thioredoxin, superoxide dismutase, and catalase levels against oxidative stress. The cell death mechanism was confirmed by mitochondrial dysfunction and decreased ATP levels, mitochondrial copy numbers, and PGC-1α expression. To further substantiate the mechanism of cell death mediated by endoplasmic reticulum stress (ERS), we determined the expression of the inositol-requiring enzyme (IRE1), (PKR-like ER kinase) PERK, activating transcription factor 6 (ATF6), and activating transcription factor 4 ATF4, the apoptotic markers p53, Bax, and caspase 3, and the anti-apoptotic marker Bcl-2. PtNPs could activate ERS and apoptosis mediated by mitochondria. A proinflammatory response to PtNPs was confirmed by significant upregulation of interleukin-1-beta (IL-1β), interferon γ (IFNγ), tumor necrosis factor alpha (TNFα), and interleukin (IL-6). Transcriptomic and molecular pathway analyses of THP-1 cells incubated with the half maximal inhibitory concentration (IC50) of PtNPs revealed the altered expression of genes involved in protein misfolding, mitochondrial function, protein synthesis, inflammatory responses, and transcription regulation. We applied transcriptomic analyses to investigate anisotropic PtNP-induced toxicity for further mechanistic studies. Isotropic nanoparticles are specifically used to inhibit non-specific cellular uptake, leading to enhanced in vivo bio-distribution and increased targeting capabilities due to the higher radius of curvature. These characteristics of anisotropic nanoparticles could enable the technology as an attractive platform for nanomedicine in biomedical applications.

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“…The PtNPs exhibited poor cellular apoptosis possibly due to the fact that PtNPs have anti-oxidative stress properties similar to those of cellular antioxidant enzymes (Adeyemi et al, 2018a). Nevertheless, studies have demonstrated in vitro apoptotic tendency of PtNPs in cancer cells, human fibroblast as well as in bacteria cells (Bendale et al, 2017;Kutwin et al, 2017; bacterial cells and HFF cells (Nejdl et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Cellular apoptosis of HFF cells by inorganic nanoparticles not susceptible to modulation by Toxoplasma gondii infection in vitro

Adeyemi

Otohinoyi

Awakan

et al. 2019

Toxicology in Vitro

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The interaction of nanoparticles with living cells is becoming one of the urgent areas of collaborative research in materials science and biology. Previously, we showed that nanoparticles have promising anti-Toxoplasma gondii properties. Meanwhile, Toxoplasma gondii has been shown to avert apoptosis in host cells whereas nanoparticles have been implicated for apoptotic tendency. Therefore, in the present study, we assessed the in vitro apoptotic properties of inorganic nanoparticles in the absence or presence of Toxoplasma infection and/or small molecules used as metabolic modulators. Results showed that inorganic nanoparticles dose-dependently caused cellular apoptosis. However, in the presence of infection by Toxoplasma gondii, nanoparticles-induced cellular apoptosis was not mitigated. Likewise, use of several small molecules (anti-metabolites) as metabolic modulators either mildly or nearly failed to abate cellular apoptosis by nanoparticles. Taken together, our findings do not only confirm the apoptotic potential of inorganic nanoparticles but show evidence that cellular apoptosis by inorganic nanoparticles of gold and silver might not be susceptible to modulation by Toxoplasma gondii infection. The findings are new and contribute to deepen our understanding of the cellular interaction of nanoparticles.

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Investigation of platinum nanoparticle properties against U87 glioblastoma multiforme

Cited by 44 publications

References 34 publications

In vitro and in vivo assessment of biocompatibility of AZ31 alloy as biliary stents: a preclinical approach

In vitro and in vivo assessment of biocompatibility of AZ31 alloy as biliary stents: a preclinical approach

Anisotropic Platinum Nanoparticle-Induced Cytotoxicity, Apoptosis, Inflammatory Response, and Transcriptomic and Molecular Pathways in Human Acute Monocytic Leukemia Cells

Cellular apoptosis of HFF cells by inorganic nanoparticles not susceptible to modulation by Toxoplasma gondii infection in vitro

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