Investigation of telomere lengths measurement by quantitative real-time PCR to predict age

Hewakapuge, Sudinna Kulangana; Oorschot, Roland van; Lewandowski, Paul; Baindur-Hudson, Swati

doi:10.1016/j.legalmed.2008.01.007

Cited by 54 publications

(63 citation statements)

References 27 publications

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“…These results were consistent with the previous observations of Guan et al (2007), Hewakapuge et al (2008) and Kimura et al (2008) who confirmed that the length of telomeres decreased with age. Figura et al (2009) concluded that telomere shortening occurs as a consequence of proliferation along with each cell division, because of the endreplication problem of DNA-polymerase.…”

Section: Discussionsupporting

confidence: 94%

“…In contrast Baird et al (2005;Hewakapuge et al, 2008;Karlsson et al, 2008) confirmed that age estimation based on telomere shortening measured with real-time PCR is not appropriate for age estimation in forensic practice, as there was a relatively large variation between individuals, e.g., in the study of Karlsson et al (2008), R 2 = 0.3 with standard error of age prediction estimated to be much higher as ±22 years, while in the study of Hewakapuge et al (2008), R 2 value was 0.037 which was significantly low.…”

Section: Discussionmentioning

confidence: 87%

“…Ren et al (2009) in China who recorded a correlation coefficient "r" value = -0.913 and p<0.01, Hewakapuge et al (2008) explained the differences in the observed "r" values as a result of differences in ethnicity, sample size, laboratory conditions in each study and not be due to differences in measuring TL in different methods.…”

Section: Discussionmentioning

confidence: 92%

“…Various studies reported different correlation coefficient "r" values of this significant negative correlation between TL and age; Nordfjall et al (2005) in Sweden (r = -0.233, p<0.01); Hewakapuge et al (2008) in Australia (r = -0.185, p<0.05), Hoffmann et al (2009) in Germany (r = -0.601, p<0.05) and Zubakov et al (2010) in Netherlands (r = -0.987, p<0.01), all used a quantitative real-time PCR method to measure the TL.…”

Section: Discussionmentioning

confidence: 98%

“…It would be useful for the scientists to predict the outward appearance of an individual such as age, height, ethnicity, hair and skin color, by analyzing the DNA left behind in a crime scene to narrow down the range of suspects (Hewakapuge et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Telomere Length Measurement by Quantitative Real-time PCR: A Molecular Marker for Human Age Prediction

Alhusseini¹,

Madboly²

2016

American Journal of Biochemistry and Biotechnology

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The fact that telomere shortening occurs with aging leads to the hypothesis that a correlation could be made between Telomere Length (TL) and suspect age. This study aimed to assess if TL can be used as an investigational tool to predict human age through developing a formula based on this correlation with accuracy suitable to be applied in forensic practice. A quantitative real-time PCR study was carried out on 80 Egyptians, ranging in age from 1-79 years. The results confirmed that the relative TL significantly shortened with aging; "r" = -0.903 (p<0.001). The human age could be determined by the following formula: (Y = 66.9-28X), (Y: Age in years; X: Relative TL), with a regression analysis between relative TL and age had an R 2 = 0.815. The standard error of age estimate was ±10.14 years. The present study concluded that estimation of human age based on the relative TL measured by quantitative real-time PCR may be a useful method for age prediction, especially when there is no morphologic information in the biological samples, but the estimated standard error of age prediction in this study was quite high (±10 years) to be used with certainty in forensic investigations. Inter-individual variations in TL and variability among the gender must be also considered when applying this method. So, this method could only give a rough estimation of age and it may be a complementary method for age estimation from soft tissues.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Telomere Length Measurement by Quantitative Real-time PCR: A Molecular Marker for Human Age Prediction

Alhusseini¹,

Madboly²

2016

American Journal of Biochemistry and Biotechnology

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Association of shorter telomere length with essential hypertension in Indian population

Bhupatiraju

Saini

Patkar

et al. 2012

American J Hum Biol

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Telomere length analysis from minimally‐invasively collected samples: Methods development and meta‐analysis of the validity of different sampling techniques

Rej

Bondy

Lin

et al. 2020

American J Hum Biol

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Objectives Telomeres are the protective caps of chromosomes. They shorten with cell replication, age, and possibly environmental stimuli (eg, infection and stress). Short telomere length (TL) predicts subsequent worse health. Although venous whole blood (VWB) is most commonly used for TL measurement, other, more minimally invasive, sampling techniques are becoming increasingly common due to their field‐friendliness, allowing for feasible measurement in low‐resource contexts. We conducted statistical validation work for measuring TL in dried blood spots (DBS) and incorporated our results into a meta‐analysis evaluating minimally invasive sampling techniques to measure TL. Methods We isolated DNA extracts from DBS using a modified extraction protocol and tested how they endured different shipping conditions and long‐term cryostorage. We then included our in‐house DBS TL validation statistics (correlation values with VWB TL and age) in a series of meta‐analyses of results from 24 other studies that published similar associations for values between TL measured in DBS, saliva, and buccal cells. Results Our modified DBS extraction technique produced DNA yields that were roughly twice as large as previously recorded. Partially extracted DBS DNA was stable for 7 days at room temperature, and still provided reliable TL measurements, as determined by external validation statistics. In our meta‐analysis, DBS TL had the highest external validity, followed by saliva, and then buccal cells—possibly reflecting similarities/differences in cellular composition vs VWB. Conclusions DBS DNA is the best proxy for VWB from the three minimally‐invasively specimen types evaluated and can be used to expand TL research to diverse settings and populations.

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Investigation of telomere lengths measurement by quantitative real-time PCR to predict age

Cited by 54 publications

References 27 publications

Telomere Length Measurement by Quantitative Real-time PCR: A Molecular Marker for Human Age Prediction

Telomere Length Measurement by Quantitative Real-time PCR: A Molecular Marker for Human Age Prediction

Association of shorter telomere length with essential hypertension in Indian population

Telomere length analysis from minimally‐invasively collected samples: Methods development and meta‐analysis of the validity of different sampling techniques

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