Investigation of the interaction between modified ISCOMs and stratum corneum lipid model systems

Abstract

The modified ISCOMs, so-called Posintro nanoparticles, provide an opportunity for altering the surface charge of the particles, which influences their affinity for the negatively charged antigen sites, cell membranes and lipids in the skin. Hypothetically, this increases the passage of the ISCOMs (or their components) and their load through the stratum corneum. The subsequent increase in the uptake by the antigen-presenting cells results in enhanced transcutaneous immunization. To understand the nature of penetration of Posintro nanoparticles into the intercorneocyte space of the stratum corneum, the interaction between the nanoparticles and lipid model systems in form of liposomes and/or supported lipid bilayer was studied. As a lipid model we used Stratum Corneum Lipid (SCL), a mixture similar in composition to the lipids of the intercorneocyte space. By Förster Resonance Energy Transfer (FRET), Atomic Force Microscopy (AFM), Electrochemical Impedance Spectroscopy (EIS) and cryo-Transmission Electron Microscopy (cryo-TEM) it was shown that application of nanoparticles to the SCL bilayers results in lipid disturbance. Investigation of this interaction by means of Isothermal Titration Calorimetry (ITC) confirmed existence of an enthalpically unfavorable reaction. All these methods demonstrated that the strength of electrostatic repulsion between the negatively charged SCL and the nanoparticles affected their interaction, as decreasing the negative charge of the Posintro nanoparticles leads to enhanced disruption of lipid organization.

Structure of Immune Stimulating Complex Matrices and Immune Stimulating Complexes in Suspension Determined by Small-Angle X-Ray Scattering

“…The reproducibility of the preparation method was confirmed by low interbatch variability as evaluated by DLS size measurements. These observations correspond to data obtained with the same components and preparation procedure (29,30).…”

Ultrathin, Ultrasmooth Gold Layer on Dielectrics without the Use of Additional Metallic Adhesion Layers

“…The surface treatment consists in immersing a silicon wafer in a solution of 95 % of Isopropyl Alcohol, 2.5 % of water and 2.5 % of silane for three hours [14,15,16,13]. We performed several tests to find the optimum immersion time.…”

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

“…Protein/peptide interactions with nucleic acids Lipid/membrane interactions Polysaccharide interactions …”

“…(vii) Lipid/membrane interactions. [325][326][327][328][329][330][331][332][333][334][335][336][337][338][339][340][341] (viii) Polysaccharide interactions. (ix) Protein/peptide interactions with polymers and nanoparticles.…”