Investigation on the binding site in heparin by spectrophotometry

Jiao, Qingcai; Liu, Qingchen; Sun, Changshan; He, Hua

doi:10.1016/s0039-9140(98)00330-0

Cited by 68 publications

(49 citation statements)

References 19 publications

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“…However, most efforts have been made to investigate the binding of dyes with heparin. [7][8][9][10][11][12][13][14] Binding studies of dyes with chondroitin have been much fewer and a profound investigation is necessary to elucidate the interaction. The main techniques involved in studying the interaction between dye and CHS [15][16][17][18][19] were absorption spectroscopy and conductometric titration for the sake of sensitivity and convenience.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic Study on the Interaction between Methylene Blue and Chondroitin 4-Sulfate and Its Analytical Application

Zhang

Zhao

et al. 2004

ANAL. SCI.

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The interaction of Methylene Blue (MB) with chondroitin-4-sulfate (CHS) has been investigated using spectroscopic techniques, including UV-Vis absorption, Rayleigh resonance scattering (RRS), and circular dichroism (CD). The addition of CHS caused a decrease in the absorbance of MB at 664 nm with a new absorption band appearing at 570 nm, enhanced RRS at 314 nm and 560 nm, and also resulted in an intense CD signal at 568 nm. The Scatchard model has been applied to calculating the binding constant and the number of binding sites. The calculated parameters are consistent with the experimental results. The factors affecting the interaction were investigated. Quantitative spectroscopic methods were developed for the first time. They are based on the fact that a decrease in the absorption at 664 nm and an enhancement of the RRS intensity at 314 nm are proportional to the concentration of CHS added in a certain range. Satisfactory results were obtained on the determination of synthetic samples.

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Section: Introductionmentioning

confidence: 99%

Spectroscopic Study on the Interaction between Methylene Blue and Chondroitin 4-Sulfate and Its Analytical Application

Zhang

Zhao

et al. 2004

ANAL. SCI.

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“…Similar changes in the absorption spectra of phenothiazine dye (in this case, methylene blue) in water have been reported as being due to complex formation with negatively charged polysaccharides possessing a sulfate group. 44,45 Analytical performances of the HRP/Th-CF-based flow-H2O2-biosensor Based on these results described above, we selected the adsorption conditions as being optimum (Th, 0.5 mM; HRP, 0.033 mg/ml; adsorption solvent, pure water (pH 6.8), adsorption time, 24 h).…”

Section: Absorbance Spectra Of Th Without and With Hrpmentioning

confidence: 99%

Carbon-felt-based Bioelectrocatalytic Flow-detectors: Optimization of the Adsorption Conditions of Horseradish Peroxidase and Thionine onto Carbon-felt for Highly Sensitive Amperometric Determination of H2O2

Wang

Hasebe

2011

ANAL. SCI.

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Horseradish peroxidase (HRP) and thionine (Th) were co-adsorbed onto a porous carbon-felt (CF) from a mixed solution of HRP and Th to fabricate a CF-based bioelectrocatalytic flow-detector for H2O2. The resulting HRP and Th co-adsorbed CF (HRP/Th-CF) was successfully used as a working electrode unit of a highly sensitive amperometric flow-H2O2-biosensor. Differing from the ordinary mediator-based HRP biosensors, which detect mediated currents (i.e., the reduction current of the oxidized form of the mediators), the present HRP/Th-CF-based H2O2-biosensor directly detects the reduction current of the oxidative HRP-intermediates (i.e., compound I and II) based on a direct electron transfer (DET) between a HRP-redox active heme center and the CF electrode, because co-adsorbed Th together with HRP facilitate the DET. Various adsorption conditions (i.e., HRP concentration, Th concentration, ionic strength, pH, adsorption time) were optimized to obtain excellent sensor performances. When an air-saturated 0.1 M phosphate buffer (pH 7.0) was used as a carrier at 3.25 ml/min and the applied potential of -0.05 V vs. Ag/AgCl, the HRP/Th-CF-based flow-H2O2-biosensor exhibited excellent analytical performances (i.e., sensitivity, 3.55 μA/μM; linear range, 0.1 to 10 μM; lower detection limit, 0.03 μM; K app m , 36.2 μM; Imax, 158 μM; sample throughput, ca. 60 samples/h).

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“…Comprehensive studies of a variety of cationic surfactants with anionicpolyelectrolytes, have been reported [26][27][28][29][30][31][32]. Cationic dyes that interacted with Heparin mostly fell in the Thiazine group such as MethyleneBlue [33][34][35]. Azure A [36,37], Azure B [38,39].…”

Section: Introductionmentioning

confidence: 99%

A Study of Effect of Dye Structure on Polyelectrolyte Induced Metachromasy

Ratnakar¹

2014

SJC

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Abstract:The interaction of two cationic dyes, namely, Azure B (AB) and Pinacyanol chloride (Pcyn) with an anionic polyelectrolyte, namely, sodium carrageenate (NaCar) has been investigated by Spectrophotometric method. The polymer induced metachromasy in the dyes resulting in the shift of the absorption maxima of the dyes towards shorter wavelengths. The stability of the complexes formed between Azure B and sodium carrageenate was found to be lesser than that formed between Pinacyanol chloride and sodium carrageenate. This fact was further confirmed by reversal studies using alcohols, urea surfactants and electrolytes. The interaction parameters revealed that binding between Azure B and sodium carrageenate was mainly due to electrostatic interaction while that between Pinacyanol chloride and carrageenate is found to involve both electrostatic and hydrophobic forces. The effect of the structure of the dye and its relation to metachromasy has been discussed.

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Investigation on the binding site in heparin by spectrophotometry

Cited by 68 publications

References 19 publications

Spectroscopic Study on the Interaction between Methylene Blue and Chondroitin 4-Sulfate and Its Analytical Application

Spectroscopic Study on the Interaction between Methylene Blue and Chondroitin 4-Sulfate and Its Analytical Application

Carbon-felt-based Bioelectrocatalytic Flow-detectors: Optimization of the Adsorption Conditions of Horseradish Peroxidase and Thionine onto Carbon-felt for Highly Sensitive Amperometric Determination of H2O2

A Study of Effect of Dye Structure on Polyelectrolyte Induced Metachromasy

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