Investigation on the conformational changes of bovine serum albumin in a wide pH range from 2 to 12

Chi, Zhenxing; Hong, Bowen; Ren, Xiulian; Cheng, Kunyu; Lu, Yuqian; Liu, Xinning

doi:10.1080/00387010.2018.1471092

Cited by 30 publications

(18 citation statements)

References 34 publications

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“…2 B, water has a strong IR absorbance around 1640 cm –1 (H–O–H bending), which overlaps with the Amide I mode of BSA (1700 cm –1 and 1600 cm –1 ). Despite these experimental challenges, we succeeded in acquiring BSA spectra over the pH range of interest here that could be used to characterize the BSA secondary structure because of the high signal/noise provided by the ATR-FTIR setup, as well previous studies 6 , 44 , 46 , 48 , 90 , 91 .…”

Section: Resultsmentioning

confidence: 99%

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The molecular insights into protein adsorption on hematite surface disclosed by in-situ ATR-FTIR/2D-COS study

Barreto

Elzinga

Alleoni

2020

Sci Rep

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Proteins are of ubiquitous interest in the Life Sciences but are of interest in the Geosciences as well because of the significant role these compounds play in the biogeochemical cycling of trace and nutrient elements. Structural changes resulting from the adsorption of proteins onto mineral surfaces may alter protein biological function and other environmental interactions. Iron oxides are major sinks of a range of environmental elements including organic compounds. In this study, the adsorption of the broadly studied model protein BSA onto the hematite mineral surface was characterized as a function of pH, ionic strength, and BSA concentration using in-situ Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy. BSA lost the α-helix and gain β-sheets in the secondary structure during adsorption on hematite. BSA adsorption was maximum at pH 5, a value close to the BSA isoelectric point (~ pH 5), and lower at pH 4 and pH 7. Increasing ionic strength decreased to total BSA adsorption. Two-dimensional correlation spectroscopy analysis of the ATR-FTIR spectra revealed that higher initial BSA concentration and the consequent higher BSA surface loading enhanced BSA adsorption by protein–protein interaction, which less ordered structures changes into more compact forms decrease, hence compacting the structural arrangement and could promoting multilayers/aggregation formation on the mineral surface. The activity of enzymes following adsorption on mineral surfaces requires further study.

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Section: Resultsmentioning

confidence: 99%

“…Secondary structure can be determined by IR spectroscopy, because structural arrangements in the protein chain are associated with specific vibrational bands 38 – 46 . Besides IR, other techniques used to determine structure include X-ray crystallography 47 and UV-circular dichroism 46 , 48 – 50 .…”

Section: Introductionmentioning

confidence: 99%

The molecular insights into protein adsorption on hematite surface disclosed by in-situ ATR-FTIR/2D-COS study

Barreto

Elzinga

Alleoni

2020

Sci Rep

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“…The content of α helix was also calculated using molecular residue ellipticity (MRE) with OriginPro 2017 (OriginLab, Northampton, MA, USA). The content of α helix was estimated based on Equations (3) and (4) (similarly as in [11,39]),…”

Section: Methodsmentioning

confidence: 99%

“…There are several different approaches used for the evaluation of alpha helix content of a protein based on CD spectra changes. In Table 1, the results of two of them are summarized: Either the CD spectra evaluated by BeStSel algorithm (detailed structural changes shown in Table S1 (Supporting information)), or calculated values based on Equations (3) and (4) mentioned in the Experimental section (similarly as in [11,39]). Both approaches confirmed the reduction of α-helicity and simultaneous increase of other structures (like turn and random coil) in the course of AuNCs formation.…”

Section: Bsa Structural Changes In the Course Of Auncs Synthesismentioning

confidence: 99%

“…Table 1. Calculated α-helix content in albumin either by using BeStSel program (detailed structural changes shown in Table S1 (Supporting information)), or MRE values as in [11,39]. The changes of α-helix content in the course of AuNCs synthetic procedure and data for each type of BSA used in our study are listed.…”

Section: Bsa Structural Changes In the Course Of Auncs Synthesismentioning

confidence: 99%

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The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

et al. 2020

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Fluorescent gold nanoclusters (AuNCs) are envisaged as a novel type of fluorophores. This work reports on the first comparative study investigating the effect of presence/absence/abundance of fatty acids (namely palmitic acid, PA) or other substances (like glycoproteins and globulins) in the protein (bovine serum albumin, BSA) on synthesis and properties of the final AuNCs. The most popular template (BSA) and microwave (MW)-assisted synthesis of AuNCs have been intentionally chosen. Our results clearly demonstrate that the fluorescent characteristics (i.e., fluorescence lifetime and quantum yield) are affected by the fatty acids and/or other substances. Importantly, the as-prepared AuNCs are biocompatible, as determined by Alamar Blue assay performed on Hep G2 cell line.

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Structured pattern hollow fiber membrane designed via reverse thermally induced phase separation method for ultrafiltration applications

Huyen

Ray

Kim

et al. 2022

J of Applied Polymer Sci

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Membrane fouling is a major problem that hinders the application of the membrane in water filtration. To address this issue, a novel reversed thermally induced phase separation (RTIPS) process is applied to fabricate a patterned polyethersulfone (PES) hollow fiber (HF) membrane using a structured spinneret. Surface patterning could induce turbulence, thereby preventing the accumulation of foulants on membrane surface. The RTIPS method requires lesser material with similar mechanical strength compared to that of conventional TIPS method. The fabrication process is optimized by changing the spinning conditions. A dope composition of 24 wt% PES is chosen to prepare the membrane. The chemical composition of the membrane is confirmed via sophisticated techniques such as Fourier‐transform infrared (FTIR) spectroscopy and X‐ray photoelectron spectroscopy (XPS). Scanning electron microscopy (SEM) images of the sample indicates the successful formation of the pattern on the shell side of the HFs. The prepared patterned HF membranes exhibits a high rejection of 97% of bovine serum albumin (BSA), which is comparable to or higher than that of commercial membranes. Moreover, the patterned membrane demonstrates better performance, thereby confirming the effectiveness of this modification in enhancing the antifouling nature.

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Investigation on the conformational changes of bovine serum albumin in a wide pH range from 2 to 12

Cited by 30 publications

References 34 publications

The molecular insights into protein adsorption on hematite surface disclosed by in-situ ATR-FTIR/2D-COS study

The molecular insights into protein adsorption on hematite surface disclosed by in-situ ATR-FTIR/2D-COS study

The Effect of Fatty Acids and BSA Purity on Synthesis and Properties of Fluorescent Gold Nanoclusters

Structured pattern hollow fiber membrane designed via reverse thermally induced phase separation method for ultrafiltration applications

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