Investigation the interaction between protamine sulfate and CdTe quantum dots with spectroscopic techniques

Abstract

The fluorescence of QDs was quenched intensely by protamine sulfate with a combined dynamic and static quenching mechanism was proposed.

The interactions of CdTe quantum dots with serum albumin and subsequent cytotoxicity: the influence of homologous ligands

“…Thermodynamics of the interactions of QDs with BSA UV-vis absorption spectroscopy is a very simple and applicable method to explore the structural changes and complex formations. 38 Thus, the absorption spectra of BSA, QDs and BSA-QD mixture were examined to further distinguish static and dynamic quenching. Dynamic quenching is largely caused by collision and merely affects the excited state of a fluorophore (BSA) and no variations occur in the absorption spectrum of the fluorophore, but a BSA-QD complex often forms during the static process and the UV-vis absorption spectrum changes.…”

Probing the Effect of Ag2S Quantum Dots on Human Serum Albumin Using Spectral Techniques

“…Fluorescence lifetime measurement can provide useful information about the type of molecular interactions, and it is the most effective method to distinguish static and dynamic quenching [10]. Figure 5 shows the fluorescence decay curves of HSA in the absence and presence of Ag 2 S QDs.…”

“…where is the gas constant, 8.314 J mol −1 K −1 ; is the temperature (K); is the equilibrium constant at the corresponding temperature, which stands for the static quenching constant ( SV ) in the present paper [10]. According to (3) and 4for plotting the ln ∼ (1/ ) diagram [33], the value of Δ was calculated, and then Δ and Δ were estimated based on the slope of the curve (Table 2).…”

“…Wang et al investigated the effects of CdSe/ZnS QDs on human blood serum and found that a hard protein corona was formed by irreversible binding [9]. In our recent study, the interaction between CdTe QDs and protamine sulfate has been examined, and a combined dynamic and static quenching model was proposed [10]. Li et al found that TGAcapped CdTe QDs could induce DNA damage and apoptosis of hepatocyte line HL-7702 [11].…”

Fluorescence sensor for detecting protamines based on competitive interactions of polyacrylic acid modified with sodium 4-amino-1-naphthalenesulfonate with protamines and aminated graphene oxide

“…Fluorescence methods are the most promising alternative methods for detecting protamines because they offer advantages over other techniques, including their speed, ease of use, and sensitivities. [9][10][11][12][13] A large number of uorescence probes have been developed for sensitively detecting protamine, but, to the best of our knowledge, only a few methods have been developed in which protamine is detected using uorescence turn-on sensors. 11,12 Fluorescence turn-on sensors offer advantages over uorescence quenching sensors in that there is less chance of false positive signals being obtained and sensitivities and selectivities are better using uorescence turn-on sensors than using uorescence quenching sensors, as has been demonstrated in numerous studies.…”