Investigations in Serum Copper. IV. Effect of Different Anions on the Enzymatic Activity of Coeruloplasmin.

Studies on Copper Metabolism. Ix. The Transportation of Copper in Blood 1

“…The specific protein or proteins to which this fraction of the copper is bound are not known. Copper combined with serum albumin or with the ft, metal-binding globulin is likewise not dialyzable within the physiologic pH range 4 and reacts directly with carbamate. Therefore, it is possible that the direct-reacting copper fraction in plasma is bound to either or both of these proteins.…”

“…This blue protein, ceruloplasmin, is an a-globulin with a molecular weight of approximately 151,000 and contains eight atoms of copper. Ceruloplasmin has true oxidase activity and since it acts on paraphenylene diamine and polyphenols but not on monophenols and monoamines, it has been classified as a laccase (3,4). Most if not all of the copper in normal human or pig serum is stated to be present in the form of this blue protein.…”

Exchange of Ceruloplasmin Copper With Ionic CU64 With Reference to Wilson's Disease1

“…In our experiments, ascorbic acid was used instead of paraphenylenediamine, the substrate toward which ceruloplasmin is most active (14), because considerable difficulty was experienced in freeing solutions of the colored products of ppd oxidation by means of ion exchange columns. The ionic composition selected was close to that under which ceruloplasmin most rapidly catalyzes the oxidation of ppd, that is, "in acetate buffers between pH 5 and 6" (20). The ionic copper concentration was chosen to be equal to the ceruloplasmin copper concentration for simplicity.…”

Studies on Copper Metabolism. Xiv. Copper, Cerulo-Plasmin and Oxidase Activity in Sera of Normal Human Subjects, Pregnant Women, and Patients With Infection, Hepatolenticular Degeneration and the Nephrotic Syndrome 1

“…A high degree of correlation was observed between the total serum copper, indirect-reacting copper, ceruloplasmin, and oxidase activity. 3. Mean values in seven patients with chronic infections were as follows: total serum copper, 192 ,Mg per 100 ml.…”

“…The substance has been shown to exhibit oxidase activity with a variety of substrates. The enzyme is most active with paraphenylene diamine (2,3).…”