Investigative Bronchoprovocation and Bronchoscopy in Airway Diseases

Busse, William W.; Wanner, Adam; Adams, Kelly M.; Reynolds, Herbert Y.; Castro, Mario; Chowdhury, Badrul A.; Kraft, Monica; Levine, Robert J.; Peters, Stephen P.; Sullivan, Eugene J.

doi:10.1164/rccm.200407-966ws

Cited by 75 publications

(57 citation statements)

References 108 publications

(131 reference statements)

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“…Fiberoptic endobronchial tumor sampling is a safe, short, and standard procedure (15). Endobronchial tumors were visualized and sampled by brushing with a 1-mm brush.…”

Section: Endobronchial Biopsymentioning

confidence: 99%

Tumor Survivin Is Downregulated by the Antisense Oligonucleotide LY2181308: A Proof-of-Concept, First-in-Human Dose Study

Talbot¹,

Ranson²,

Davies³

et al. 2010

Clin Cancer Res

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Purpose: Enhanced tumor cell survival through expression of inhibitors of apoptosis (IAP) is a hallmark of cancer. Survivin, an IAP absent from most normal tissues, is overexpressed in many malignancies and associated with a poorer prognosis. We report the first-in-human dose study of LY2181308, a secondgeneration antisense oligonucleotide (ASO) directed against survivin mRNA.Patients and Methods: A dose-escalation study evaluating the safety, pharmacokinetics, and pharmacodynamics of LY2181308 administered intravenously for 3 hours as a loading dose on 3 consecutive days and followed by weekly maintenance doses. Patients were eligible after signing informed consent, had exhausted approved anticancer therapies and agreed to undergo pre-and posttreatment tumor biopsies to evaluate reduction of survivin protein and gene expression.Results: A total of 40 patients were treated with LY2181308 at doses of 100 to 1,000 mg. Twenty-six patients were evaluated at the recommended phase 2 dose of 750 mg, at which level serial tumor sampling and

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“…Fiberoptic endobronchial tumor sampling is a safe, short, and standard procedure (15). Endobronchial tumors were visualized and sampled by brushing with a 1-mm brush.…”

Section: Endobronchial Biopsymentioning

confidence: 99%

Tumor Survivin Is Downregulated by the Antisense Oligonucleotide LY2181308: A Proof-of-Concept, First-in-Human Dose Study

Talbot¹,

Ranson²,

Davies³

et al. 2010

Clin Cancer Res

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“…Most adverse effects observed in asthma patients submitted to bronchial thermoplasty are usually seen after bronchoscopic procedures. (18)(19)(20) In this case, there were no significant complications. After each of the three sessions of treatment, the patient was monitored in the recovery room.…”

Section: Case Reportmentioning

confidence: 54%

Termoplastia brônquica: relato do primeiro tratamento endoscópico de asma na América Latina

Rubin

Cardoso

2008

J. bras. pneumol.

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Bronchial thermoplasty is a new bronchoscopic procedure that delivers radiofrequency energy to the airway and potentially reduces the smooth muscle-mediated bronchoconstriction. We report the case of a 48-year-old man with persistent moderate asthma submitted to bronchial thermoplasty. The treatment increased the forced expiratory volume in one second, increased the number of symptom-free days, reduced the use of relief medications, and improved the Juniper Asthma Quality of Life Scale score. In this patient, bronchial thermoplasty was well tolerated and safe. This was the first bronchial thermoplasty performed in Latin America. At 12 months after the procedure, the results were encouraging in terms of its potential benefits in patients with difficult-to-control asthma.

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“…A fiber optic bronchoscope (BF-P10; Olympus, Philadelphia, PA) was cleaned with a detergent (Asepti-Zyme; Ecolab, St. Paul, MN) by wiping and suction channel aspiration, followed by immersion for an hour. A sterile water rinse and air drying were performed, after which right lower lobe bronchoscopy (without lidocaine) and BAL were performed according to the guidelines of the American Thoracic Society and Busse et al (21).…”

Section: Methodsmentioning

confidence: 99%

Abundant DNase I-Sensitive Bacterial DNA in Healthy Porcine Lungs and Its Implications for the Lung Microbiome

Pezzulo

Kelly

Nassar

et al. 2013

Appl Environ Microbiol

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Human lungs are constantly exposed to bacteria in the environment, yet the prevailing dogma is that healthy lungs are sterile. DNA sequencing-based studies of pulmonary bacterial diversity challenge this notion. However, DNA-based microbial analysis currently fails to distinguish between DNA from live bacteria and that from bacteria that have been killed by lung immune mechanisms, potentially causing overestimation of bacterial abundance and diversity. We investigated whether bacterial DNA recovered from lungs represents live or dead bacteria in bronchoalveolar lavage (BAL) fluid and lung samples in young healthy pigs. Live bacterial DNA was DNase I resistant and became DNase I sensitive upon human antimicrobial-mediated killing in vitro. We determined live and total bacterial DNA loads in porcine BAL fluid and lung tissue by comparing DNase I-treated versus untreated samples. In contrast to the case for BAL fluid, we were unable to culture bacteria from most lung homogenates. Surprisingly, total bacterial DNA was abundant in both BAL fluid and lung homogenates. In BAL fluid, 63% was DNase I sensitive. In 6 out of 11 lung homogenates, all bacterial DNA was DNase I sensitive, suggesting a predominance of dead bacteria; in the remaining homogenates, 94% was DNase I sensitive, and bacterial diversity determined by 16S rRNA gene sequencing was similar in DNase I-treated and untreated samples. Healthy pig lungs are mostly sterile yet contain abundant DNase I-sensitive DNA from inhaled and aspirated bacteria killed by pulmonary host defense mechanisms. This approach and conceptual framework will improve analysis of the lung microbiome in disease.

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Investigative Bronchoprovocation and Bronchoscopy in Airway Diseases

Cited by 75 publications

References 108 publications

Tumor Survivin Is Downregulated by the Antisense Oligonucleotide LY2181308: A Proof-of-Concept, First-in-Human Dose Study

Tumor Survivin Is Downregulated by the Antisense Oligonucleotide LY2181308: A Proof-of-Concept, First-in-Human Dose Study

Termoplastia brônquica: relato do primeiro tratamento endoscópico de asma na América Latina

Abundant DNase I-Sensitive Bacterial DNA in Healthy Porcine Lungs and Its Implications for the Lung Microbiome

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