Involvement of hydrogen peroxide in heat shock- and cadmium-induced expression of ascorbate peroxidase and glutathione reductase in leaves of rice seedlings

Expression analysis of antioxidant genes in response to drought stress in the fl ag leaf of two Indonesian rice cultivars

“…The function of the APX enzyme in fl ag leaf of Situ the was regulated by three Apx genes. The previous studies found that the activity of APX enzyme in the leaf of rice plant treated to abiotic stress may be associated with the expression of Apx genes Chou et al, 2012). Therefore, the Apx genes played a major role in the defense system of Situ Bagendit were chl-sApx, cApx b then cApx a .…”

“…To determine the expression profi le of the rice antioxidant genes, semi-quantitative r e v e r s e t r a n s c r i p t i o n -p o l y m e r a s e chain reaction (sq-RT-PCR) assay was conducted using the published specific primer sequences (Kim et al, 2004;Hong et al, 2009;Chou et al, 2012). The primers used in this research were: For each sample, 5 μL PCR products were added with 1 mL loading dye (Promega), electrophoresed in a 1% syberseen (Promega)-agarose gels buffered with 1X Tris-Boric-EDTA (1X TBE), and visualized under UV light.…”

H2O2 pretreated rice seedlings specifically reduces arsenate not arsenite: difference in nutrient uptake and antioxidant defense response in a contrasting pair of rice cultivars

“…Primarily, H 2 O 2 functions as a messenger in ethylene and salicylic acid (SA) signal transduction pathway during plant stress response Chamnogpol et al 1998). Several studies have highlighted the role of H 2 O 2 in enhancing plant's tolerance towards abiotic stresses (Murphy et al 2002;Uchida et al 2002;Azevedo Neto et al 2005;Wahid et al 2007;Liu et al 2010;Chou et al 2012). At the molecular level H 2 O 2 regulates expression of several genes encoding antioxidants, defense proteins, kinase signaling proteins, phosphatases and transcription factors that are individually as well as contentedly involved in plant defense and hypersensitive reactions (Kovtun et al 2000;Hung et al 2005).…”

“…Alleviation of heavy metals induced oxidative stress have also been demonstrated during Cd treatment in rice, barley, maize, pea seedlings, Arabidopsis thaliana and Glycine max (Pal et al 2002;Metwally et al 2003;Choudhury and Panda 2004;Drazic and Mihailovic 2005;Noriega et al 2012;Guo et al 2007;Zawoznik et al 2007;Popova et al 2009;Xiao-Juan et al 2010). Reduction of Pb and Hg induced membrane damage in Oryza sativa have been studied by pretreatment with SA (Mishra and Choudhuri 1999), pre-treatement of a rice cultivar with H 2 O 2 has been reported to increase its tolerance towards Cd toxicity by increasing GSH, phytochelatins and nonprotein thiols (Chou et al 2012). However, the role of H 2 O 2 or both H 2 O 2 and SA, towards alleviation of heavy metal(loid) induced toxicity in plants has not been extensively studied.…”

Soil cadmium toxicity and nitrogen deposition differently affect growth and physiology in Toxicodendron vernicifluum seedlings

“…The presence of Cd in the soil may induce inhibition of photosynthesis (Küpper et al 2007;Chen et al 2011), and also the generation of reactive oxygen species (ROS) and inhibition or sometimes stimulation in the activity of several antioxidative enzymes, e.g. decreases in superoxidase and catalase activities, and increases in guaiacol peroxidase, glutathione reductase and ascorbate peroxidase activities (Wang et al 2008;Chou et al 2012).…”