Involvement of Polyamines with Adventitious Root Development in Stem Cuttings of Mung Bean

Jarvis, Basil; Shannon, P. R. M.; Yasmin, S.

doi:10.1093/oxfordjournals.pcp.a076564

Cited by 55 publications

(44 citation statements)

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“…For this purpose, we looked at changes in polyamine levels in hormone-treated cuttings in the presence and absence of leaves. Polyamines have been implicated in vegetative rooting of stem cuttings in different species [22,23,[26][27][28]. Thus, consistent with previous reports, putrescine levels significantly increased in the bitter melon cuttings that were about to root; i.e., cuttings with one intact leaf and treated with hormone [23,27,28].…”

Section: Discussionsupporting

confidence: 88%

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Malik¹,

Pérez²,

Kunta³

2012

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The study was conducted to investigate the optimal hormone treatment for rooting in bitter melon and the effect of defoliation on rooting and polyamine levels. Commercial preparation (diluted 1:10 and 1:20) gave extensive rooting within five days after treatment. The presence of leaf with the stem cutting was necessary for optimal rooting as defoliation drastically reduced rooting even when the rooting time was extended from 5 to 10 days. The presence of leaf also promoted the levels of putrescine three days after the hormone treatment just at the time of root emergence. Cuttings that did not have leaf did not show an increase in putrescine levels and did not produce roots. Spermidine levels also increased in the cuttings that rooted but there was no change in the levels of spermine in the cuttings that rooted. It appears that putrescine and spermidine play a role in regulating rooting in bitter melon cuttings. The vegetative propagation conditions described show that it is a practically viable technique for small growers to produce large number of plantlets at the farm for selection and cultivation.

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Section: Discussionsupporting

confidence: 88%

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Malik¹,

Pérez²,

Kunta³

2012

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“…Other results (10) showed a close relationship between rhizogenesis, ODC activity, and increase in endogenous Put and Spd levels in leaf explants of Datura innoxia cultivated in vitro in the presence of IAA. Jarvis et al (18) showed that spermine supplied alone or in the presence of IBA enhanced the number of adventitious roots formed on mung bean cuttings. In addition, IBA led to enhanced levels of PAs prior to the development of root primordia.…”

Section: Analysis Of Hydroxycinnamoylputrescine Levelsmentioning

confidence: 99%

“…In addition, IBA led to enhanced levels of PAs prior to the development of root primordia. An inhibition of root formation was obtained with different inhibitors of Put, Spd, and Spm synthesis such as canavanine, canaline, and methylglyoxal-bis (guanylhydrazone) (14,18,25). Schwartz et al (27) reported an increase in ADC and ODC activities during lateral root formation in Zea mays and showed that ODC was localized mainly in the meristematic root tip zones.…”

Section: Analysis Of Hydroxycinnamoylputrescine Levelsmentioning

confidence: 99%

Polyamines, Hydroxycinnamoylputrescines, and Root Formation in Leaf Explants of Tobacco Cultivated in Vitro

Burtin¹,

Martin-Tanguy²,

Paynot³

et al. 1990

Plant Physiol.

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In vitro formation of roots is obtained directly, without intermediate growth of callus, from foliar explants of a tobacco (Nicotiana tabacum) plant cultured on Murashige and Skoog medium containing IAA. Auxin-induced root formation was accompanied by significant changes in hydroxycinnamoylputrescine levels. Increasing levels were found in leaf explants during the first 14 days in culture; this was followed by a sharp decline after 20 days. Early changes in putrescine conjugates were detected in leaf explants before the visible appearance of roots. An early and transitory accumulation of hydroxycinnamoylputrescines was observed in the roots. Free polyamines (putrescine, spermidine, and spermine) in leaf explants and roots were always at a low level and only small changes in their concentrations were observed, a-DL-difluoromethylarginine and a-DL-difluoromethylomithine, specific, irreversible inhibitors of arginine decarboxylase and ornithine decarboxylase, respectively, inhibited putrescine accumulation and root initiation and reduced the fresh and dry weights of leaf explants. These effects were reversed by free putrescine or hydroxycinnamoylputrescines. The results reported here suggest that hydroxycinnamoylputrescines are associated with root formation. The relationship among free polyamines, hydroxycinnamoylputrescines, cell division, and root formation is discussed. (10,14,25) suggest that polyamines are involved in auxin-induced root formation, although their specific role has yet to be elucidated in order to establish a causal relationship.Amides formed between hydroxycinnamic acids and amines are widely distributed in the plant kingdom (9,20,21,28). They are detected in high amounts in flowering parts ofvarious plants and have been linked to the process offlower formation (for reviews, see refs. 20 and 31). Other factors leading to an increase in conjugated polyamines are fungal and viral infections, (for reviews see refs. 20 and 31). Recent results (8,22) report that a change in morphology or growth of tobacco leaf explants in vitro is accompanied by changes in putrescine levels, and the largest changes occur in conjugated putrescine content specifically.Are polyamines and conjugated polyamines esssential for root formation, or are they simply secondary by-products of a growth response? One way to approach this question is by utilizing specific inhibitors of polyamine synthesis and then examining the physiological properties of tissues.Previous reports have shown that in tobacco plants putrescine is synthesized from arginine (by ADC' via agmatine) and ornithine (by ODC).This study reports results of DFMA, a specific enzymeactivated ADC inhibitor, and DFMO, a specific enzymeactivated ODC inhibitor, on organogenesis, growth, free polyamine, and conjugated polyamine (hydroxycinnamoylputrescine) levels of tobacco leaf explants cultivated in vitro in a medium that induces root formation. MATERIALS AND METHODS Biological Material and Growth ConditionsNicotiana tabacum var Xanthi n.c. was utilized in ...

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“…'application exogene de Spm (50 pM) augmcnte le nombre de racines adventives initiees en presence ou en absence de l'acide indol butyrique (AlB) (Jarvis et al, 1983). La Spd exogene a forte concentration n'a pas d'effet sur !…”

Section: Enracincmcntunclassified

“…Chez les vegetaux, les polyamines semblent jouer un role important dans de multiples processus physiologiques du developpement : division cellulaire (Heimer et al, 1979), developpement racinaire (Jarvis et al, 1983), embryogenese somatique (Montague et al, 1979), flo raison (Cabanne et al, 1977 ;Martin-Tanguy et al, 1984), croissance du tube pollinique (Bagni et al, 1981), germination (Smith, 1979), senescence (Altman et al, 1977;Galston et al, 197&) et reponse a un stress (Young et Gals ton 1983 ;Slocum et al, 1984 ;Tiburcio et al, 1986 ;Nadeau et al, 1987).…”

Section: Introductionunclassified

Métabolisme et rôle des polyamines dans le développement de la plante

Daoudi¹,

Biondi

1995

Acta Botanica Gallica

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Summary.-Aliphatic polyamines, putrescine, spermidine and spermine are ubiquHous in all living organisms. They occur in the free form as cations, but are often conjugated to small molecule like phenolics acids and also to various macromolecules. Polyamines and their biosynthetic enzymes may play an imponant role in many aspects of plants development including growth, differentiation, senescence and response to dillerents types of stress. Recent worl< suggest, but has not proven, a regulatory role of polyamines in these processes. Putrescine accumulation in response to stress, suggests a protective role. By contrast, increased spermidine and spermine synthesis appears essential for DNA replication and cell division.

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Involvement of Polyamines with Adventitious Root Development in Stem Cuttings of Mung Bean

Cited by 55 publications

References 0 publications

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Polyamines, Hydroxycinnamoylputrescines, and Root Formation in Leaf Explants of Tobacco Cultivated in Vitro

Métabolisme et rôle des polyamines dans le développement de la plante

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